Construct: ORF TRCN0000474208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011991.1_s317c1
- Derived from:
- ccsbBroadEn_07482
- DNA Barcode:
- CCCAGGGATGCGTGTCCGCGGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNPH (9751)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9751 | SNPH | syntaphilin | NM_014723.4 | 99.9% | 100% | 18A>T |
| 2 | human | 9751 | SNPH | syntaphilin | XM_005260889.3 | 93.6% | 93.7% | 18A>T;48_146del |
| 3 | human | 9751 | SNPH | syntaphilin | XM_011529404.2 | 93.6% | 93.7% | 18A>T;48_146del |
| 4 | human | 9751 | SNPH | syntaphilin | NM_001318234.2 | 91.7% | 91.8% | 1_132del;150A>T |
| 5 | human | 9751 | SNPH | syntaphilin | XM_011529402.2 | 86.4% | 86.5% | 1_132del;150A>T;180_278del |
| 6 | human | 9751 | SNPH | syntaphilin | XM_011529403.2 | 86.4% | 86.5% | 1_132del;150A>T;180_278del |
| 7 | mouse | 241727 | Snph | syntaphilin | NM_001291076.1 | 87.6% | 90.7% | (many diffs) |
| 8 | mouse | 241727 | Snph | syntaphilin | NM_198214.3 | 82.1% | 85.1% | (many diffs) |
| 9 | mouse | 241727 | Snph | syntaphilin | XM_011239538.1 | 82.1% | 85.1% | (many diffs) |
| 10 | mouse | 241727 | Snph | syntaphilin | XM_017318258.1 | 81.3% | 76.9% | (many diffs) |
| 11 | mouse | 241727 | Snph | syntaphilin | NM_001291077.1 | 80.9% | 83.7% | (many diffs) |
| 12 | mouse | 241727 | Snph | syntaphilin | XM_017318256.1 | 80.5% | 83.3% | (many diffs) |
| 13 | mouse | 241727 | Snph | syntaphilin | XM_011239535.1 | 75.8% | 78.6% | (many diffs) |
| 14 | mouse | 241727 | Snph | syntaphilin | XM_011239536.1 | 75.8% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1548
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catgtccctg cctggaagta gacggacctc tgctggatca cgcaggcgca 121 cctctccacc tgtgagcgtg cgggatgcct acggcacctc ttcgctcagc agcagcagca 181 attctggctc ctacaagggc agtgacagca gtcccacgcc aaggcgctcc atgaaataca 241 cgctgtgcag tgacaaccat ggcatcaagc ccccgacccc ggagcagtac ctgacccccc 301 tgcagcagaa ggaggtgtgc atccggcacc tgaaagcccg gctgaaggac acacaggacc 361 ggctccagga ccgggacaca gagattgatg acctgaagac gcagctgtca cgcatgcagg 421 aggactggat tgaggaggag tgccaccgcg tggaggccca gctggccctg aaggaggccc 481 gaaaggagat caagcagctc aagcaggtca tcgacactgt caagaacaac ctgattgaca 541 aggacaaggg gctgcagaag tacttcgtgg acatcaacat ccagaacaag aagctggaga 601 cgctgctgca cagcatggag gtggcccaga atggcatggc caaggaggat ggcactgggg 661 agtcagccgg tgggtcccct gcccgctccc tcacccgcag ctccacctac accaagctga 721 gtgacccggc tgtctgtggt gaccgccagc cgggtgatcc ctccagcggc tctgctgagg 781 atggggcaga cagtggcttt gcagcagccg atgacacact gagccggacg gacgcgctgg 841 aagccagcag cctgctgtcg tcgggggtgg actgtggcac cgaggagacc tcgctgcaca 901 gctccttcgg cctgggcccc cgcttccctg ccagcaacac ctatgagaag ctgctgtgtg 961 gcatggaggc tggtgtgcag gccagctgca tgcaggagcg tgccatccag acagacttcg 1021 tgcagtacca gcctgacctt gacaccatcc tggagaaagt gacccaggcc caggtctgtg 1081 ggacagaccc tgAGTCAGGG GACAGGTGCC CAGAGCTGGA TGCCCACCCT TCAGGGCCCA 1141 GAGACCCCAA CTCAGCAGTG GTGGTGACAG TGGGTGATGA GCTAGAGGCC CCAGAGCCCA 1201 TCACCCGTGG ACCCACCCCA CAGCGGCCTG GTGCCAACCC CAACCCTGGC CAGTCGGTGA 1261 GCGTGGTGTG CCCCATGGAA GAGGAGGAGG AGGCTGCCGT GGCTGAGAAG GAGCCCAAGA 1321 GCTACTGGAG CCGCCACTAC ATCGTGGATC TGCTGGCTGT GGTGGTGCCG GCCGTGCCCA 1381 CGGTGGCCTG GCTTTGCCGC TCCCAGCGGC GCCAGGGCCA GCCCATCTAC AACATCAGCT 1441 CCCTGCTGCG GGGCTGCTGC ACTGTGGCCT TGCACTCCAT CCGCAGGATC AGCTGCCGCT 1501 CGCTGAGCCA GCCGAGTCCC AGCCCAGCGG GCGGCGGCTC CCAGCTCTGC CCAACTTTCT 1561 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1621 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1681 GTGGAAAGGA CGACCCAGGG ATGCGTGTCC GCGGATTACG CGTTAAGTCg acaatcaacc 1741 tctggattac aaaatttgtg aaagatt