Construct: ORF TRCN0000474222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016036.1_s317c1
- Derived from:
- ccsbBroadEn_13131
- DNA Barcode:
- GACTCCGTATCCCGATTCGTATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAH14 (127602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474222
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | NM_001145154.3 | 96.6% | 96.6% | 1_45del |
| 2 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | NM_001349911.2 | 91.6% | 91.6% | 1_45del;580_581ins69 |
| 3 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | NM_001367481.1 | 56.4% | 52.4% | (many diffs) |
| 4 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | NM_144989.3 | 46.6% | 45.1% | (many diffs) |
| 5 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | NM_001349912.2 | 42% | 42.1% | 1_45del;215_216ins150;618_618delGins592 |
| 6 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_017000298.1 | 11.2% | 11.2% | 1_45del;1359_11664delinsG |
| 7 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544072.2 | 9.8% | 8.9% | (many diffs) |
| 8 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544070.2 | 9.7% | 9.7% | 1_45del;1359_13473delinsG |
| 9 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544069.2 | 9.7% | 9.7% | 1_45del;1359_13497delinsG |
| 10 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544067.2 | 9.6% | 9.6% | 1_45del;1359_13623delinsG |
| 11 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544066.2 | 9.6% | 9.6% | 1_45del;1359_13635delinsG |
| 12 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544065.2 | 9.6% | 9.6% | 1_45del;1359_13659delinsG |
| 13 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544064.2 | 9.5% | 9.6% | 1_45del;1359_13683delinsG |
| 14 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544063.2 | 9.5% | 9.5% | 1_45del;1359_13692delinsG |
| 15 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544062.2 | 9.5% | 9.5% | 1_45del;1359_13695delinsG |
| 16 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_017000297.1 | 9.5% | 9.5% | 1_45del;1359_13734delinsG |
| 17 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544061.2 | 9.5% | 9.5% | 1_45del;1359_13746delinsG |
| 18 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_017000296.1 | 9.5% | 9.5% | 1_45del;1359_13752delinsG |
| 19 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_006711735.3 | 9.5% | 9.5% | 1_45del;1359_13758delinsG |
| 20 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_017000295.1 | 9.5% | 9.5% | 1_45del;1359_13776delinsG |
| 21 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544059.2 | 9.5% | 9.5% | 1_45del;1359_13803delinsG |
| 22 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544058.2 | 9.4% | 9.4% | 1_45del;1359_13848delinsG |
| 23 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | NM_001367479.1 | 9.4% | 9.4% | 1_45del;1359_13851delinsG |
| 24 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XM_011544071.2 | 8.6% | 6.6% | (many diffs) |
| 25 | human | 127602 | DNAH14 | dynein axonemal heavy chain 14 | XR_001736974.1 | 8.5% | 1_1491del;2805_15345delinsG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1383
- ORF length:
- 1314
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacaaggag gaaaccaaga caaaaccaag acttttaaga tatgaagaga 121 aaaaatatga agatgtgaaa ccattagaga ctcaaccagc tgaaatagca gaaaaggaaa 181 cattggaata taaaacagtt agaacattct ctgaatcttt gaagtcagag aaaacagaag 241 attaccttag agaaagtata attcaacaac atatggtttc tccagagcca gcttccctta 301 aggagaaagg gaagtcaagg agaaaaaagg atcaaactca tgcttgtcca aatgttagga 361 aagccaggcc tgtgtcctat gatagaacag aaccaaaaga tgatgatgtg ataagaaata 421 ttattaggct acgagaaaag cttggttggc aaactatatt accgcagcac agtttgaaat 481 acggaagctc caaaattgca attcagaaga ttactttaaa gaaacctttg gaagatgatg 541 gagaatttgt ttattgcctt cctcggaaaa gtcctaaatc cctttacaat ccatatgatc 601 ttcaggtagt atcggctcat actgctaaac attgcaaaga attttgggtt attactgctt 661 catttatctc aaaggttatt aatatagttg gtagtgtaaa ggaagtagaa ctcataccta 721 ctttggaatg gctatcagaa agaagacatt actatttatt acggcaattc aagatatttt 781 ctgatttccg aatgaataaa gcatttgtta cctGGAAATT GAATGTTAAA AGAATTAAGA 841 CAGAGAAGAG CAGGTCATTT TTGTACCACC ATCTTTTTTT GGCTGATGAC TTGTTTCAAA 901 CCTGTTTGGT TTATATAAGA GGACTTTGTG AAGATGCAAT TAATCTCAAA AATTATAATG 961 ACCATGAAAA TAATCTATCT GCCATATGCC TTGTAAAGCT GGATAGTTCT CGAACATATT 1021 CTCTAGATGA ATTTTGTGAA GAGCAGTTAC AGCAAGCTAC CCAGGCATTG AAACAACTTG 1081 AGGACATCAG GAATAAAGCA ATTTCAGAGA TGAAAAGTAC TTTTCTAAAG GTTGCAGAAA 1141 AGAATGAAAT CAAAGAGTAT TTTGAGTCAA AACTCTCTGA AGATGACACA ACACATTTCA 1201 AGCTGCCTAA ATATAGACGT TTATTAGAAA CATTTTTCAA GTTTGTAATG CTGGTTGACT 1261 ACATATTTCA GGAACTCATT CGTCAACTTA TGAACACTGC AGTCACACTA CTTTTGGAAT 1321 TATTTAATGG TTCTGCTGGA ATGCCATTTT CAGTGGAAAA AAAGAATGAA AATCTTATCA 1381 GGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGAGA CTCCGTATCC CGATTCGTAT ACACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt