Construct: ORF TRCN0000474246
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006353.1_s317c1
- Derived from:
- ccsbBroadEn_08959
- DNA Barcode:
- AGGCGCAAACGACTTAACGGACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL36 (79786)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474246
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79786 | KLHL36 | kelch like family member 36 | NM_024731.4 | 99.8% | 99.8% | 631C>T;1270T>C |
| 2 | human | 79786 | KLHL36 | kelch like family member 36 | XM_005256149.2 | 99.8% | 99.8% | 631C>T;1270T>C |
| 3 | human | 79786 | KLHL36 | kelch like family member 36 | NM_001303451.2 | 89.7% | 89.7% | 631C>T;1104_1105ins189 |
| 4 | human | 79786 | KLHL36 | kelch like family member 36 | XR_001751986.2 | 24% | (many diffs) | |
| 5 | human | 79786 | KLHL36 | kelch like family member 36 | XR_933433.3 | 19.9% | (many diffs) | |
| 6 | mouse | 234796 | Klhl36 | kelch-like 36 | NM_146219.1 | 85.9% | 85.5% | (many diffs) |
| 7 | mouse | 234796 | Klhl36 | kelch-like 36 | XR_001778433.1 | 56.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1914
- ORF length:
- 1848
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggagggaagc aggcagacgc gagtgtctcg gccatacaag atcagcgaat 121 catcaaaggt ataccgctgg gccgaccact caagcacggt gctgcagcgg ctgaacgagc 181 agcgtctccg cgggctcttc tgcgacgtcg tcctggtggc cgatgagcag cgtgtgccag 241 cccatcgcaa cctgctggcc gtgtgcagcg actacttcaa ctccatgttc accatcggca 301 tgcgggaagc tttccagaag gaggtggagc tgatcggcgc ctcctacatt gggctcaagg 361 ccgtggtgga cttcctgtac ggcggggagc tggtgctgga tggcggcaac attgactacg 421 tcctggagac ggctcacctg ctgcagatct ggacggtggt agacttctgc tgtgagtacc 481 tggagcagga ggtgagcgag gacaactacc tgtacctgca ggagctggcc tccatctaca 541 gcctcaagcg gcttgatgcc ttcatcgatg gcttcatcct gaaccacttc ggcacgctgt 601 cctttacgcc cgacttcctg cagaacgtct ccatgcagaa gctgtgtgtc tacctgagca 661 gcagcgaggt gcagcgggag tgtgagcacg acctcttgca ggccgccctg cagtggctga 721 cgcagcagcc cgagcgcgag gcccacgccc gccaggtgct ggagaacatc cacttcccgc 781 tcatccccaa gaacgacctg ctgcaccgcg tcaagccggc cgtgtgctcg ctgctgccca 841 aggaggccaa ctgcgagggc ttcatcgagg aggccgtgcg ctaccacaac aacctggcgg 901 cccagcccgt catgcagacc aagcgcacgg cgctgcgcac caaccaggag cgcctgctgt 961 ttgtgggcgg cgaggtctcc gagcggtgtc tggagctcag tgacgacacc tgctacctgg 1021 acgccaagag cgagcagtgg gtcaaagaga cgccgctgcc cgcccggcgg agccaccact 1081 gtgtcgcggt gctggggggc ttcatcttca tcgccggcgg cagcttctca cgggacaacg 1141 gaggggatgc ggcctccaat cttctttata ggtatgaccc ccgctgtaaa cagtggatca 1201 aggtggcctc catgaaccag cgccgtgtgg atttctacct tgcctccatc gaagacatgc 1261 tggtggccat cggcggccgg aatgagaacg gagcgctctc ttcagtagag acgtacagtc 1321 ccaagactga ctcccggtcc tatgtggccg gcttgccaag gttcacgtac ggccacgcgg 1381 gcaccatcta caaagacttc gtgtacatct cggggggcca cgactaccaa attggcccct 1441 accgcaagaa cctgctatgc tacgaccacc ggacagacgt gtgggaggag cggcggccca 1501 tgaccacggc gcgcggctgg cacagcatgt gcagccTGGG TGACAGCATC TACTCCATCG 1561 GGGGCAGCGA TGACAACATC GAGTCCATGG AGCGCTTCGA CGTGCTGGGC GTGGAGGCCT 1621 ACAGCCCGCA GTGCAACCAG TGGACCCGCG TGGCGCCGCT GCTGCACGCC AACAGCGAGT 1681 CGGGCGTGGC AGTGTGGGAG GGCCGCATCT ACATCCTGGG CGGCTACAGC TGGGAGAACA 1741 CTGCCTTCTC CAAGACCGTG CAGGTGTACG ACCGCGAGGC CGACAAGTGG AGCAGGGGCG 1801 TCGACCTGCC CAAGGCCATC GCTGGCGGGT CCGCCTGTGT CTGCGCCCTG GAGCCACGGC 1861 CAGAGGACAA GAAGAAGAAA GGCAAAGGCA AGAGGCACCA GGACCGGGGC CAGTGCCCAA 1921 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1981 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2041 TATCTTGTGG AAAGGACGAA GGCGCAAACG ACTTAACGGA CCGACGCGTT AAGTCgacaa 2101 tcaacctctg gattacaaaa tttgtgaaag att