Construct: ORF TRCN0000474246

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006353.1_s317c1
Derived from:
ccsbBroadEn_08959
DNA Barcode:
AGGCGCAAACGACTTAACGGACCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLHL36 (79786)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474246
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79786 KLHL36 kelch like family member 36 NM_024731.4 99.8% 99.8% 631C>T;1270T>C
2 human 79786 KLHL36 kelch like family member 36 XM_005256149.2 99.8% 99.8% 631C>T;1270T>C
3 human 79786 KLHL36 kelch like family member 36 NM_001303451.2 89.7% 89.7% 631C>T;1104_1105ins189
4 human 79786 KLHL36 kelch like family member 36 XR_001751986.2 24% (many diffs)
5 human 79786 KLHL36 kelch like family member 36 XR_933433.3 19.9% (many diffs)
6 mouse 234796 Klhl36 kelch-like 36 NM_146219.1 85.9% 85.5% (many diffs)
7 mouse 234796 Klhl36 kelch-like 36 XR_001778433.1 56.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1914
ORF length:
1848
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat ggagggaagc aggcagacgc gagtgtctcg gccatacaag atcagcgaat
121catcaaaggt ataccgctgg gccgaccact caagcacggt gctgcagcgg ctgaacgagc
181agcgtctccg cgggctcttc tgcgacgtcg tcctggtggc cgatgagcag cgtgtgccag
241cccatcgcaa cctgctggcc gtgtgcagcg actacttcaa ctccatgttc accatcggca
301tgcgggaagc tttccagaag gaggtggagc tgatcggcgc ctcctacatt gggctcaagg
361ccgtggtgga cttcctgtac ggcggggagc tggtgctgga tggcggcaac attgactacg
421tcctggagac ggctcacctg ctgcagatct ggacggtggt agacttctgc tgtgagtacc
481tggagcagga ggtgagcgag gacaactacc tgtacctgca ggagctggcc tccatctaca
541gcctcaagcg gcttgatgcc ttcatcgatg gcttcatcct gaaccacttc ggcacgctgt
601cctttacgcc cgacttcctg cagaacgtct ccatgcagaa gctgtgtgtc tacctgagca
661gcagcgaggt gcagcgggag tgtgagcacg acctcttgca ggccgccctg cagtggctga
721cgcagcagcc cgagcgcgag gcccacgccc gccaggtgct ggagaacatc cacttcccgc
781tcatccccaa gaacgacctg ctgcaccgcg tcaagccggc cgtgtgctcg ctgctgccca
841aggaggccaa ctgcgagggc ttcatcgagg aggccgtgcg ctaccacaac aacctggcgg
901cccagcccgt catgcagacc aagcgcacgg cgctgcgcac caaccaggag cgcctgctgt
961ttgtgggcgg cgaggtctcc gagcggtgtc tggagctcag tgacgacacc tgctacctgg
1021acgccaagag cgagcagtgg gtcaaagaga cgccgctgcc cgcccggcgg agccaccact
1081gtgtcgcggt gctggggggc ttcatcttca tcgccggcgg cagcttctca cgggacaacg
1141gaggggatgc ggcctccaat cttctttata ggtatgaccc ccgctgtaaa cagtggatca
1201aggtggcctc catgaaccag cgccgtgtgg atttctacct tgcctccatc gaagacatgc
1261tggtggccat cggcggccgg aatgagaacg gagcgctctc ttcagtagag acgtacagtc
1321ccaagactga ctcccggtcc tatgtggccg gcttgccaag gttcacgtac ggccacgcgg
1381gcaccatcta caaagacttc gtgtacatct cggggggcca cgactaccaa attggcccct
1441accgcaagaa cctgctatgc tacgaccacc ggacagacgt gtgggaggag cggcggccca
1501tgaccacggc gcgcggctgg cacagcatgt gcagccTGGG TGACAGCATC TACTCCATCG
1561GGGGCAGCGA TGACAACATC GAGTCCATGG AGCGCTTCGA CGTGCTGGGC GTGGAGGCCT
1621ACAGCCCGCA GTGCAACCAG TGGACCCGCG TGGCGCCGCT GCTGCACGCC AACAGCGAGT
1681CGGGCGTGGC AGTGTGGGAG GGCCGCATCT ACATCCTGGG CGGCTACAGC TGGGAGAACA
1741CTGCCTTCTC CAAGACCGTG CAGGTGTACG ACCGCGAGGC CGACAAGTGG AGCAGGGGCG
1801TCGACCTGCC CAAGGCCATC GCTGGCGGGT CCGCCTGTGT CTGCGCCCTG GAGCCACGGC
1861CAGAGGACAA GAAGAAGAAA GGCAAAGGCA AGAGGCACCA GGACCGGGGC CAGTGCCCAA
1921CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1981ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
2041TATCTTGTGG AAAGGACGAA GGCGCAAACG ACTTAACGGA CCGACGCGTT AAGTCgacaa
2101tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)