Transcript: Human XM_005256149.2

PREDICTED: Homo sapiens kelch like family member 36 (KLHL36), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KLHL36 (79786)
Length:
7773
CDS:
370..2220

Additional Resources:

NCBI RefSeq record:
XM_005256149.2
NBCI Gene record:
KLHL36 (79786)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005256149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000179941 CGGCCTCCAATCTTCTTTATA pLKO.1 1454 CDS 100% 15.000 21.000 N KLHL36 n/a
2 TRCN0000437852 CCTTGCCTCCATCGAAGACAT pLKO_005 1542 CDS 100% 4.950 6.930 N KLHL36 n/a
3 TRCN0000150052 CCTCCAATCTTCTTTATAGGT pLKO.1 1457 CDS 100% 3.000 4.200 N KLHL36 n/a
4 TRCN0000415152 CGCTGTAAACAGTGGATCAAG pLKO_005 1486 CDS 100% 4.950 3.960 N KLHL36 n/a
5 TRCN0000183415 CAGCAGCAACTTCTTAGTATT pLKO.1 2277 3UTR 100% 13.200 9.240 N KLHL36 n/a
6 TRCN0000147189 CAGCGAATCATCAAAGGTATA pLKO.1 417 CDS 100% 10.800 7.560 N KLHL36 n/a
7 TRCN0000179898 CCAGCAGCAACTTCTTAGTAT pLKO.1 2276 3UTR 100% 5.625 3.938 N KLHL36 n/a
8 TRCN0000180247 CGATGACAACATCGAGTCCAT pLKO.1 1872 CDS 100% 0.264 0.185 N KLHL36 n/a
9 TRCN0000438451 ATGAGAACGGAGCGCTCTCTT pLKO_005 1586 CDS 100% 4.950 2.970 N KLHL36 n/a
10 TRCN0000437270 TACAGCCTCAAGCGGCTTGAT pLKO_005 841 CDS 100% 4.950 2.970 N KLHL36 n/a
11 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 7053 3UTR 100% 4.950 2.475 Y DCAF11 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005256149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08959 pDONR223 100% 99.8% 99.8% None 631C>T;1270T>C n/a
2 ccsbBroad304_08959 pLX_304 0% 99.8% 99.8% V5 631C>T;1270T>C n/a
3 TRCN0000474246 AGGCGCAAACGACTTAACGGACCG pLX_317 20.7% 99.8% 99.8% V5 631C>T;1270T>C n/a
4 ccsbBroadEn_12617 pDONR223 100% 89.7% 89.7% None 631C>T;1105_1293del n/a
5 ccsbBroad304_12617 pLX_304 0% 89.7% 89.7% V5 631C>T;1105_1293del n/a
6 TRCN0000477616 GGTCTCAATTTCGCTGGCTGCTTA pLX_317 27.1% 89.7% 89.7% V5 631C>T;1105_1293del n/a
Download CSV