Construct: ORF TRCN0000474266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000733.1_s317c1
- Derived from:
- ccsbBroadEn_14067
- DNA Barcode:
- GGCGTTAGAGCCTGAGAGGTGTGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- XRN2 (22803)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | NM_012255.5 | 99.9% | 77.3% | 2197_2198insA |
| 2 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | NM_001317960.1 | 91.6% | 69.2% | (many diffs) |
| 3 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | XM_017027723.2 | 55.3% | 32.8% | 0_1ins1272;925_926insA |
| 4 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | XM_017027722.1 | 49.4% | 58.5% | (many diffs) |
| 5 | human | 22803 | XRN2 | 5'-3' exoribonuclease 2 | XM_017027724.1 | 39.3% | 46.6% | (many diffs) |
| 6 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | NM_011917.2 | 92.2% | 75.6% | (many diffs) |
| 7 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | XM_011239519.2 | 76% | 92.4% | (many diffs) |
| 8 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | XM_006499450.3 | 75.4% | 58.3% | (many diffs) |
| 9 | mouse | 24128 | Xrn2 | 5'-3' exoribonuclease 2 | XM_006499451.2 | 49.7% | 31.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2328
- ORF length:
- 2259
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagtcccg gcgttcttcc gctggctcag ccgcaagtac ccgtccatca 121 tagtcaactg cgtggaagag aagccaaaag aatgcaatgg tgtaaagatt ccagttgatg 181 ccagtaaacc taatccaaat gatgtggagt ttgataatct gtatttggat atgaatggaa 241 tcatccatcc ctgtactcat cctgaagaca aaccagcacc aaaaaatgaa gatgaaatga 301 tggttgcaat ttttgagtac attgacagac ttttcagtat tgtaagacca agaagacttc 361 tctacatggc aatagatgga gtggcaccac gtgctaaaat gaaccagcag cgttcaagga 421 ggttcagggc atcaaaagaa ggaatggaag cagcagtcga gaagcagcga gtcagggaag 481 aaatattggc aaaaggtggc tttcttcctc cagaagaaat aaaagaaaga tttgacagca 541 actgtattac accaggaact gaattcatgg acaatcttgc taaatgcctt cgctattaca 601 tagctgatcg tttaaataat gaccctgggt ggaaaaattt gacagttatt ttatctgatg 661 ctagtgctcc tggtgaagga gaacataaaa tcatggatta cattagaagg caaagagccc 721 agcctaacca tgacccaaat actcatcatt gtttatgtgg agcagatgct gatctcatta 781 tgcttggcct tgccacacat gaaccgaact ttaccattat tagagaagaa ttcaaaccaa 841 acaagcccaa accatgtggt ctttgtaatc agtttggaca tgaggtcaaa gattgtgaag 901 gtttgccaag agaaaagaag ggaaagcatg atgaacttgc cgatagtctt ccttgtgcag 961 aaggagagtt tatcttcctt cggcttaatg ttcttcgtga gtatttggaa agagaactca 1021 caatggccag cctaccattc acatttgatg ttgagaggag cattgatgac tgggttttca 1081 tgtgcttctt tgtgggaaat gacttcctcc ctcatttgcc atcgttagag attagggaaa 1141 atgcaattga ccgtttggtt aacatataca aaaatgtggt acacaaaact gggggttacc 1201 ttacagaaag tggttatgtc aatctgcaaa gagtacagat gatcatgtta gcagttggtg 1261 aagttgagga tagcattttt aaaaagagaa aggatgatga ggacagtttt agaagacgac 1321 agaaagaaaa aagaaagaga atgaagagag atcaaccagc tttcactcct agtggaatat 1381 taactcctca tgccttgggt tcaagaaatt caccaggttc tcaagtagcc agtaatccga 1441 gacaagcagc ctatgaaatg aggatgcaga ataactctag tccttcgata tctcctaata 1501 cgagtttcac atctgatggc tccccgtctc cattaggagg aattaagcga aaagcagaag 1561 acagtgacag tgaacctgag ccagaggata atgtcaggtt atgggaagct ggctggaagc 1621 agcggtacta caagaacaaa tttgatgtgg atgcagctga tgagaaattc cgtcggaaag 1681 ttgtgcagtc gtacgttgaa ggactttgct gggttcttag atattattac cagggctgtg 1741 cttcctggaa gtggtattat ccatttcatt atgcaccatt tgcttcagac tttgaaggca 1801 ttgcagacat gccatctgat tttgagaagg gtacgaaacc gtttaaacca ctagaacaac 1861 ttatgggggt atttccagct gcaagtggta attttctacc tccatcatgg cggaagctca 1921 tgagtgatcc tgattctagt ataattgact tctatcctga agattttgct attgatttga 1981 atgggaagaa atatgcatgg caaggtgttg ctctcttgcc attcgtggat gagcgaaggc 2041 tacgagctgc cctagaagag gtatacccag acctcactcc agaagagacc agaagaaaca 2101 gccttggagg tgatgtctta tttgtgggga aacatcaccc actccatgac ttcattttag 2161 agctgtacca gacaggttcc acagagccag tggaggtacc ccctgaacta tgtcatggga 2221 ttcaaggaaa gttttctttg gatgaagaag ccattcttcc agatcaaaat agtatgttct 2281 cctgttccta tgttaaggga tctgacacag aacactgtag tcagtattaa ttttaaagac 2341 ccacagtttg ctgaagatta catttttaaa gctgtaatgc ttccaggagc aagaaagcca 2401 gcagcagtac tgaaacctag tgactgggaa aaatccagca atggacggca gtggaagcct 2461 cagcttggct ttaaccgtga ccggaggcct gtgcacctgg atcaggcagc cttcaggact 2521 ttgggccatg tgatgccaag aggcTCAGGA ACTGGCATTT ACAGCAATGC TGCACCACCA 2581 CCTGTGACTT ACCAGGGAAA CTTATACAGG CCGCTTTTGA GAGGACAAGC CCAGATTCCA 2641 AAACTTATGT CAAATATGAG GCCCCAGGAT TCCTGGCGAG GTCCTCCTCC CCTTTTCCAG 2701 CAGCAAAGGT TTGACAGAGG CGTTGGGGCT GAACCTCTGC TCCCATGGAA CCGGATGCTG 2761 CAAACCCAGA ATGCAGCCTT CCAGCCAAAC CAGTACCAGA TGCTAGCTGG GCCTGGTGGG 2821 TATCCACCCA GACGAGATGA TCGTGGAGGG AGACAGGGAT ATCCCAGAGA AGGAAGGAAA 2881 TACCCTTTGC CACCACCCTC AGGAAGATAC AATTGGAATT TGCCAACTTT CTTGTACAAA 2941 GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA 3001 TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 3061 GACGAGGCGT TAGAGCCTGA GAGGTGTGGA CGCGTTAAGT Cgacaatcaa cctctggatt 3121 acaaaatttg tgaaagatt