Construct: ORF TRCN0000474307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006360.1_s317c1
- Derived from:
- ccsbBroadEn_12905
- DNA Barcode:
- ATTCAAACGGAAAATAATATACGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- REPS1 (85021)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | XM_011536202.2 | 98.8% | 98.6% | (many diffs) |
| 2 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | XM_017011389.1 | 95.2% | 95% | (many diffs) |
| 3 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | NM_031922.4 | 93.3% | 93.2% | (many diffs) |
| 4 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | NM_001286611.1 | 93.2% | 93% | (many diffs) |
| 5 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | XM_017011387.2 | 93.2% | 93% | (many diffs) |
| 6 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | XM_005267177.4 | 93% | 92.9% | (many diffs) |
| 7 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | XM_017011388.2 | 92.5% | 90.4% | (many diffs) |
| 8 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | XM_005267179.4 | 89.9% | 89.8% | (many diffs) |
| 9 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | NM_001128617.2 | 89.8% | 89.6% | (many diffs) |
| 10 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | XM_005267178.5 | 87.4% | 85.3% | (many diffs) |
| 11 | human | 85021 | REPS1 | RALBP1 associated Eps domai... | NM_001286612.1 | 82% | 81.8% | (many diffs) |
| 12 | mouse | 19707 | Reps1 | RalBP1 associated Eps domai... | NM_009048.2 | 84.2% | 88.4% | (many diffs) |
| 13 | mouse | 19707 | Reps1 | RalBP1 associated Eps domai... | XM_017313852.1 | 84.1% | 88.3% | (many diffs) |
| 14 | mouse | 19707 | Reps1 | RalBP1 associated Eps domai... | XM_006512621.2 | 81.6% | 85.9% | (many diffs) |
| 15 | mouse | 19707 | Reps1 | RalBP1 associated Eps domai... | NM_001111065.1 | 80.8% | 85% | (many diffs) |
| 16 | mouse | 19707 | Reps1 | RalBP1 associated Eps domai... | XM_017313853.1 | 78.2% | 82.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2295
- ORF length:
- 2229
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctttgtggt gcaacaagac ttggttattt tggaagaagt cagttctaca 121 ttgctttgaa acttgtagct gttgcccagt ctggtttccc tttaagagtg gaaagtataa 181 atacagtaaa ggaccttcct ctgccaagat ttgttgcttc aaagaatgaa caggaatctc 241 gccatgcagc ctcatattct tcagattctg aaaatcaagg gtcgtattct ggtgtaattc 301 ccccaccacc tggcaggggg caagtgaaaa agggatccgt aagccatgat acggttcagc 361 ctcgtacatc tgcagatgca caggaacctg catccccagt agtttcacca cagcaatccc 421 caccaacttc tccacacaca tggaggaagc acagccgtca tcccagcggt gggaatagtg 481 agaggcctct cgcgggacct gggccatttt ggtctccctt tggtgaagca caatcaggtt 541 cttctgctgg tgatgcagtg tggtcagggc attccccacc tccacctcaa gaaaactggg 601 tcagttttgc agatactcca ccaaccagta ctcttttaac catgcatcct gcttctgtcc 661 aggaccagac aacagtacga actgtagcat cagctacaac tgccattgaa attcgtaggc 721 aatccagtag ttatgatgat ccctggaaaa taacagatga acaaagacag tattatgtaa 781 atcagtttaa aaccattcag cctgatctaa acggatttat tccaggatct gcagctaaag 841 agttttttac aaaatcaaaa cttcctattc ttgaactttc tcatatttgg gaactctcag 901 actttgataa agatggtgca ttgacactgg atgagttttg tgctgctttt catctggtgg 961 ttgctaggaa gaatggctat gatttaccag aaaaacttcc tgaaagctta atgcccaaac 1021 tgattgattt ggaagattca gcagatgttg gggatcagcc aggtgaggta ggttattcag 1081 gctctcctgc tgaagctcct ccaagcaagt caccatcgat gccatcacta aaccagacat 1141 ggcctgagct gaatcagagc agtgagcagt gggagacatt tagtgaacgc tcttcaagct 1201 cacaaactct gacccaattt gattctaaca ttgcaccagc tgatcctgat actgctattg 1261 ttcatccagt tcccattcgt atgactccaa gcaaaatcca catgcaggaa atggaactta 1321 aaagaactgg cagcgatcat acaaatccca ctagcccatt acttgtgaaa ccatctgacc 1381 ttttagaaga aaataagata aattcatcgg tgaaattcgc ttctggtaat actgtagatg 1441 gttacagtag ttcagactct tttacttctg acccagaaca gatcgggagc aatgtaactc 1501 gtcaaaggtc tcattcagga acgtcgcctg ataacactgc accaccacct ccccctccaa 1561 ggccacagcc ctctcattct agatcatcat ctttagatat gaatcggacc tttacagtca 1621 ccacaggaca acaacaggct ggagttgttg cccatcctcc tgcagtgcct ccaagaccac 1681 agccctcaca ggctcctggt cctgctgtgc atcgcccagt ggatgccgat ggcctcataa 1741 ctcacactag tacctcacct cagcagatac cagagcaacc aaattttgca gatttcagtc 1801 agtttgaagt atttgctgca tcaaatgtaa acgacgaaca agatgatgaa gccgagaaac 1861 atccagaagt cctgccggct gaaaaagctt ctgatccTGC AAGTTCTCTT CGAGTTGCCA 1921 AAACAGATAG TAAAACTGAA GAAAAGACAG CTGCTAGTGC TCCTGCCAAT GTGAGCAAAG 1981 GCACAACACC ACTTGCTCCA CCACCTAAAC CTGTTCGAAG AAGATTAAAA TCAGAAGATG 2041 AATTAAGGCC AGAAGTTGAT GAACATACAC AAAAGACGGG TGTCTTAGCT GCTGTTCTTG 2101 CATCACAACC TTCTATTCCC AGATCTGTTG GGAAAGATAA GAAAGCTATT CAGGCATCAA 2161 TTAGACGTAA TAAGGAAACC AACACCGTTT TGGCCAGATT GAATAGCGAA TTGCAGCAAC 2221 AATTAAAGGA TGTTCTTGAG GAGAGAATTT CCCTGGAAGT TCAACTGGAA CAACTTCGAC 2281 CATTTTTTCC CCTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2341 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2401 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTCAAACGG AAAATAATAT 2461 ACGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt