Construct: ORF TRCN0000474323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005417.1_s317c1
- Derived from:
- ccsbBroadEn_10787
- DNA Barcode:
- ATACGTCGTAATTGCCCCCTCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC1I2 (1781)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474323
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001271788.2 | 100% | 100% | |
2 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001271789.2 | 100% | 100% | |
3 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001271790.2 | 99.8% | 99.8% | 1723_1724insAGC |
4 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001320884.2 | 99.8% | 99.8% | 1723_1724insAGC |
5 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001271786.2 | 97.1% | 97.1% | 226_279del |
6 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001271787.2 | 97.1% | 97.1% | 226_279del |
7 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001320882.2 | 96.8% | 96.8% | 317_376del |
8 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001320883.2 | 96.8% | 96.8% | 317_376del |
9 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | XM_006712347.4 | 96.6% | 96.6% | 317_376del;1783_1784insAGC |
10 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | XM_017003525.2 | 96.6% | 96.6% | 317_376del;1783_1784insAGC |
11 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001271785.2 | 95.9% | 95.9% | 226_243del;335_394del |
12 | human | 1781 | DYNC1I2 | dynein cytoplasmic 1 interm... | NM_001378.3 | 95.9% | 95.9% | 226_243del;335_394del |
13 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001198876.1 | 91.6% | 97.8% | (many diffs) |
14 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_010064.4 | 91.6% | 97.8% | (many diffs) |
15 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_006498679.2 | 91.5% | 97.7% | (many diffs) |
16 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_017315326.1 | 91.5% | 97.7% | (many diffs) |
17 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001198875.1 | 90.7% | 96.9% | (many diffs) |
18 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_006498678.2 | 90.7% | 96.9% | (many diffs) |
19 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001198874.1 | 88.7% | 94.7% | (many diffs) |
20 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_006498677.1 | 88.7% | 94.7% | (many diffs) |
21 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_017315324.1 | 88.6% | 94.6% | (many diffs) |
22 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001347173.1 | 87.9% | 93.8% | (many diffs) |
23 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_017315319.1 | 87.9% | 93.8% | (many diffs) |
24 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_006498676.1 | 87.7% | 93.7% | (many diffs) |
25 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001198873.1 | 86.3% | 92.1% | (many diffs) |
26 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001198878.1 | 86.3% | 92.1% | (many diffs) |
27 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001198872.1 | 85.6% | 91.4% | (many diffs) |
28 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | NM_001198877.1 | 85.6% | 91.4% | (many diffs) |
29 | mouse | 13427 | Dync1i2 | dynein cytoplasmic 1 interm... | XM_006498680.3 | 71.4% | 73.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1902
- ORF length:
- 1836
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agacaaaagt gaattaaagg ctgagttgga acgtaagaag cagcgactgg 121 cccaaatcag agaggaaaag aagagaaaag aagaagaaag gaaaaaaaaa gaaacagacc 181 agaagaagga agctgttgct cctgtgcaag aagaatcaga tcttgaaaaa aaaaggagag 241 aagctgaagc attgcttcaa agcatggggc taactccaga atcccccatt gtccctcctc 301 ctatgtctcc atcctccaaa tctgtgagca ctccaagtga agctggaagc caagactctg 361 gagatggcgc cgtgggatct agacgaggac ctattaaact tggaatggct aaaatcacgc 421 aagtcgactt tcctcctcga gaaattgtca cgtatacaaa ggaaactcag actccagtta 481 tggctcaacc caaagaagat gaagaggaag atgatgatgt agtggctcct aaaccaccta 541 ttgaacctga agaagagaaa actttaaaga aagatgagga aaatgatagt aaagctcccc 601 ctcatgagct gactgaagaa gaaaagcaac aaatcttgca ctctgaggaa tttttaagtt 661 tctttgacca ttctacaaga attgtagaaa gagctctttc tgagcagatt aacatcttct 721 ttgactatag tgggagagat ttggaagaca aagaaggaga gattcaagca ggtgctaaac 781 tgtcattaaa tcgacaattt tttgacgaac gttggtcaaa gcatcgggtg gttagttgtt 841 tggattggtc atctcagtat ccggagttac tcgtggcttc ctataacaac aatgaagatg 901 cccctcatga gcctgatggt gtggcccttg tatggaatat gaaatacaaa aaaactaccc 961 cagagtatgt gtttcactgc cagtcagctg tgatgtctgc cacatttgca aaatttcatc 1021 caaatcttgt tgttggtggt acatattcag gccaaattgt gctttgggat aaccgtagca 1081 ataaaagaac tccagtgcaa agaactccac tgtcagcagc tgcacacaca caccctgtat 1141 attgtgtaaa tgttgttgga acacaaaatg ctcacaatct gattagcatc tctactgatg 1201 gaaaaatttg ttcatggagt ctggacatgc tttcccatcc acaggatagc atggagttgg 1261 ttcataaaca gtcaaaagca gtagctgtga catctatgtc cttccctgtt ggagatgtca 1321 acaactttgt tgttgggagt gaagaaggtt ctgtgtacac agcatgccgc catggcagca 1381 aagctggaat cagtgagatg tttgaggggc atcaaggacc aaTCACTGGC ATCCATTGTC 1441 ATGCAGCTGT TGGAGCAGTA GACTTCTCAC ATCTTTTTGT CACTTCATCG TTTGACTGGA 1501 CAGTAAAGCT TTGGACAACT AAGAATAACA AGCCTTTGTA TTCATTTGAA GATAATGCAG 1561 ACTATGTTTA TGATGTTATG TGGTCACCTA CCCACCCAGC CCTGTTTGCC TGTGTGGATG 1621 GCATGGGGAG ATTGGATTTG TGGAATCTCA ATAATGACAC AGAGGTACCA ACTGCCAGCA 1681 TTTCTGTGGA GGGTAATCCT GCTCTTAATC GTGTGAGATG GACCCATTCT GGCAGAGAGA 1741 TTGCTGTGGG TGATTCTGAA GGACAGATTG TTATATACGA TGTGGGAGAG CAGATTGCTG 1801 TTCCCCGCAA TGATGAATGG GCACGGTTTG GCCGAACACT TGCAGAAATT AATGCAAACC 1861 GAGCTGATGC AGAGGAGGAA GCAGCTACCC GAATACCTGC TTACCCAACT TTCTTGTACA 1921 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1981 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2041 AGGACGAATA CGTCGTAATT GCCCCCTCTA TACGCGTTAA GTCgacaatc aacctctgga 2101 ttacaaaatt tgtgaaagat t