Construct: ORF TRCN0000474347
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004404.1_s317c1
- Derived from:
- ccsbBroadEn_09673
- DNA Barcode:
- GGGACAAACCCGGACCGAGTTCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- COMMD1 (150684)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474347
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150684 | COMMD1 | copper metabolism domain co... | NM_152516.3 | 99.6% | 43.2% | 240_241insA;492T>C |
| 2 | human | 150684 | COMMD1 | copper metabolism domain co... | XM_011532558.2 | 83.7% | 51.2% | (many diffs) |
| 3 | human | 150684 | COMMD1 | copper metabolism domain co... | XM_011532559.2 | 68.4% | 11.9% | (many diffs) |
| 4 | human | 150684 | COMMD1 | copper metabolism domain co... | NM_001321781.2 | 64.9% | 8.8% | 0_1ins198;42_43insA;294T>C |
| 5 | human | 150684 | COMMD1 | copper metabolism domain co... | NM_001321782.2 | 64.9% | 8.8% | 0_1ins198;42_43insA;294T>C |
| 6 | human | 150684 | COMMD1 | copper metabolism domain co... | NM_001371765.1 | 64.9% | 8.8% | 0_1ins198;42_43insA;294T>C |
| 7 | human | 150684 | COMMD1 | copper metabolism domain co... | XM_017003412.1 | 64.9% | 8.8% | 0_1ins198;42_43insA;294T>C |
| 8 | mouse | 17846 | Commd1 | COMM domain containing 1 | NM_144514.2 | 84.6% | 38% | (many diffs) |
| 9 | mouse | 17846 | Commd1 | COMM domain containing 1 | XM_006514564.3 | 56.8% | 10% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgggcgag cttgagggtg gcaaacccct gagcgggctg ctgaatgcgc 121 tggcccagga cactttccac gggtaccccg gcatcacaga ggagctgcta cggagccagc 181 tatatccaga ggtgccaccc gaggagttcc gcccctttct ggcaaagatg agggggattc 241 ttaagtctat tgcgtctgca gacatggatt tcaaccagct GGAGGCATTC TTGACTGCTC 301 AAACCAAAAA AGCAAGGTGG GATCACATCT GACCAAGCTG CTGTCATTTC CAAATTCTGG 361 AAGAGCCACA AGACAAAAAT CCGTGAGAGC CTCATGAACC AGAGCCGCTG GAATAGCGGG 421 CTTCGGGGCC TGAGCTGGAG AGTTGATGGC AAGTCTCAGT CAAGGCACTC AGCTCAAATA 481 CACACACCTG TTGCCATTAT AGAGCTGGAA TTAGGCAAAT ATGGACAGGA ATCTGAATTT 541 CTGTGTTTGG AATTTGACGA GGTCAAAGTC AACCAAATTC TGAAGACGCT GTCAGAGGTA 601 GAAGAAAGTA TCAGCACACT GATCAGCCAG CCTAACTGCC CAACTTTCTT GTACAAAGTG 661 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 721 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 781 GAGGGACAAA CCCGGACCGA GTTCTAACGC GTTAAGTCga caatcaacct ctggattaca 841 aaatttgtga aagatt