Construct: ORF TRCN0000474361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016089.1_s317c1
- Derived from:
- ccsbBroadEn_02888
- DNA Barcode:
- CAGACAACCGACCCGATGCCGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIPSNAP3A (25934)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25934 | NIPSNAP3A | nipsnap homolog 3A | NM_015469.3 | 100% | 100% | |
| 2 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | NM_018376.4 | 91% | 87% | (many diffs) |
| 3 | human | 25934 | NIPSNAP3A | nipsnap homolog 3A | NM_001329570.2 | 79.7% | 79.7% | 428_429ins150 |
| 4 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XM_011518839.1 | 73% | 69.2% | (many diffs) |
| 5 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XR_001746344.1 | 34% | (many diffs) | |
| 6 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | NR_130760.2 | 33.8% | (many diffs) | |
| 7 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XR_001746345.1 | 32.6% | (many diffs) | |
| 8 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | XR_428532.1 | 11.2% | (many diffs) | |
| 9 | human | 55335 | NIPSNAP3B | nipsnap homolog 3B | NR_130759.2 | 8.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cgtcctcaga agcgccctga ctcgggcgct ggcctcacgg acgctggcgc 121 ctcagatgtg ctcatctttt gctacgggac ccagacaata cgatggaata ttctatgaat 181 ttcgttctta ttaccttaag ccctcaaaga tgaatgagtt cctggaaaat tttgagaaaa 241 acgctcatct tcggacagct cactctgaat tggttggata ctGGAGTGTA GAATTTGGAG 301 GCAGAATGAA TACAGTGTTT CATATTTGGA AGTATGATAA TTTTGCTCAT CGAACTGAAG 361 TTCGGAAAGC CTTGGCCAAA GATAAGGAAT GGCAAGAACA ATTCCTCATT CCAAATTTGG 421 CTCTCATTGA TAAACAAGAG AGTGAGATTA CTTATCTGGT ACCATGGTGC AAATTAGAAA 481 AACCTCCAAA AGAAGGAGTC TATGAACTGG CCACTTTTCA GATGAAACCT GGTGGGCCAG 541 CTCTGTGGGG TGATGCATTT AAAAGGGCAG TTCATGCTCA TGTCAATCTA GGCTACACAA 601 AACTAGTTGG AGTGTTCCAC ACAGAGTACG GAGCACTCAA CAGAGTTCAT GTTCTTTGGT 661 GGAATGAGAG TGCAGATAGT CGTGCAGCTG GGAGACATAA GTCCCATGAG GATCCCAGAG 721 TTGTGGCAGC TGTTCGGGAA AGTGTCAACT ACCTAGTATC TCAGCAGAAT ATGCTTCTGA 781 TTCCTACATC GTTTTCACCA CTGAAATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAGACAAC 961 CGACCCGATG CCGGATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt