Transcript: Human NM_001329570.2

Homo sapiens nipsnap homolog 3A (NIPSNAP3A), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
NIPSNAP3A (25934)
Length:
1486
CDS:
111..704

Additional Resources:

NCBI RefSeq record:
NM_001329570.2
NBCI Gene record:
NIPSNAP3A (25934)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001329570.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000143434 GCCAAAGATAAGGAATGGCAA pLKO.1 420 CDS 100% 2.640 2.112 N NIPSNAP3A n/a
2 TRCN0000122085 CAAGAGAGTGAGATTACTTAT pLKO.1 480 CDS 100% 13.200 9.240 N NIPSNAP3A n/a
3 TRCN0000122170 GAACAATTCCTCATTCCAAAT pLKO.1 441 CDS 100% 10.800 7.560 N NIPSNAP3A n/a
4 TRCN0000121935 CTTTGTAAGTACCACTTCAAA pLKO.1 855 3UTR 100% 5.625 3.938 N NIPSNAP3A n/a
5 TRCN0000121880 GCTTTGTAAGTACCACTTCAA pLKO.1 854 3UTR 100% 4.950 3.465 N NIPSNAP3A n/a
6 TRCN0000142425 GCCTCAGATGTGCTCATCTTT pLKO.1 164 CDS 100% 0.563 0.394 N NIPSNAP3A n/a
7 TRCN0000144290 CCACATTGTCATTTGTGACAT pLKO.1 1212 3UTR 100% 0.495 0.347 N NIPSNAP3A n/a
8 TRCN0000144608 GAAAGTGTCAACTACCTAGTA pLKO.1 633 CDS 100% 4.950 2.475 Y NIPSNAP3A n/a
9 TRCN0000144213 CAGCAGAATATGCTTCTGATT pLKO.1 657 CDS 100% 0.495 0.248 Y NIPSNAP3A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001329570.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02888 pDONR223 100% 79.7% 79.7% None 428_429ins150 n/a
2 ccsbBroad304_02888 pLX_304 0% 79.7% 79.7% V5 428_429ins150 n/a
3 TRCN0000474361 CAGACAACCGACCCGATGCCGGAT pLX_317 70.3% 79.7% 79.7% V5 428_429ins150 n/a
4 ccsbBroadEn_03584 pDONR223 100% 73% 69.2% None (many diffs) n/a
5 ccsbBroad304_03584 pLX_304 0% 73% 69.2% V5 (many diffs) n/a
6 TRCN0000481287 CCCAGGGAGACTGACGTTACTGCA pLX_317 58.6% 73% 69.2% V5 (many diffs) n/a
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