Construct: ORF TRCN0000474365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003890.1_s317c1
- Derived from:
- ccsbBroadEn_04773
- DNA Barcode:
- TAGACGGGCCTAACCTCCAGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIPA1 (123606)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 123606 | NIPA1 | NIPA magnesium transporter 1 | NM_001142275.1 | 100% | 100% | |
| 2 | human | 123606 | NIPA1 | NIPA magnesium transporter 1 | NM_144599.5 | 77.2% | 77.2% | 1_225del |
| 3 | mouse | 233280 | Nipa1 | non imprinted in Prader-Wil... | NM_153578.2 | 71.9% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 828
- ORF length:
- 762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgttggccag attggaaact tcctggctta cacggcggtc cccacggtcc 121 tggtaacccc cctgggcgcc cttggagtac cgttcgggtc cattttagct tcctatctcc 181 tgaaggaaaa gctcaacatc ttgggcaagt tggggtgcct gctaagctgt gcaggctccg 241 tcgtgctgat tatccactcc ccaaagtctg agagtgtgac aactcaggct gagctggagg 301 aaaagctgac CAATCCAGTG TTTGTGGGCT ACCTGTGCAT CGTGCTGCTC ATGCTGCTGC 361 TGCTCATCTT CTGGATCGCG CCGGCCCATG GGCCCACCAA CATCATGGTC TACATCAGCA 421 TCTGCTCCTT GCTGGGCAGT TTCACCGTGC CTTCCACCAA GGGCATCGGG CTGGCGGCCC 481 AAGACATCTT GCATAACAAC CCGTCCAGTC AGAGAGCCCT CTGCCTGTGC CTGGTACTCC 541 TGGCCGTGCT CGGCTGCAGC ATCATCGTCC AGTTCAGGTA CATCAACAAG GCGCTGGAGT 601 GCTTCGACTC CTCGGTGTTC GGGGCCATCT ACTACGTCGT GTTTACCACG CTGGTCCTGC 661 TGGCCTCAGC CATCCTCTTC CGGGAGTGGA GCAACGTGGG CCTGGTGGAC TTCTTGGGGA 721 TGGCCTGTGG ATTCACGACC GTCTCCGTGG GGATTGTCCT TATACAGGTG TTCAAAGAGT 781 TCAATTTCAA CCTTGGGGAG ATGAACAAAT CTAATATGAA AACAGACTAC CCAACTTTCT 841 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 901 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 961 GTGGAAAGGA CGATAGACGG GCCTAACCTC CAGGAACACG CGTTAAGTCg acaatcaacc 1021 tctggattac aaaatttgtg aaagatt