Construct: ORF TRCN0000474369

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014061.1_s317c1
Derived from:
ccsbBroadEn_06034
DNA Barcode:
CATCCACTGCTGTACAACGAGACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CPT1B (1375)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474369
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1375 CPT1B carnitine palmitoyltransfer... NM_001145135.2 99.9% 99.8% 931A>G
2 human 1375 CPT1B carnitine palmitoyltransfer... NM_001145137.2 99.9% 99.8% 931A>G
3 human 1375 CPT1B carnitine palmitoyltransfer... NM_004377.4 99.9% 99.8% 931A>G
4 human 1375 CPT1B carnitine palmitoyltransfer... NM_152245.3 99.9% 99.8% 931A>G
5 human 1375 CPT1B carnitine palmitoyltransfer... NM_152246.3 99.9% 99.8% 931A>G
6 human 1375 CPT1B carnitine palmitoyltransfer... NM_001145134.2 95.5% 95.4% 459_460ins102;829A>G
7 human 386593 CHKB-CPT1B CHKB-CPT1B readthrough (NMD... NR_027928.2 53.7% (many diffs)
8 mouse 12895 Cpt1b carnitine palmitoyltransfer... NM_009948.2 85.8% 86.9% (many diffs)
9 mouse 100134861 Gm44502 predicted readthrough trans... NR_004843.2 41.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2385
ORF length:
2316
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggaagct caccaggccg tggccttcca gttcacggtg accccagacg
121gggtcgactt ccggctcagt cgggaggccc tgaaacacgt ctacctgtct gggatcaact
181cctggaagaa acgcctgatc cgcatcaaga atggcatcct caggggcgtg taccctggca
241gccccaccag ctggctggtc gtcatcatgg caacagtggg ttcctccttc tgcaacgtgg
301acatctcctt ggggctggtc agttgcatcc agagatgcct ccctcagggg tgtggcccct
361accagacccc gcagacccgg gcacttctca gcatggccat cttctccacg ggcgtctggg
421tgacgggcat cttcttcttc cgccaaaccc tgaagctgct tctctgctac catgggtgga
481tgtttgagat gcatggcaag accagcaact tgaccaggat ctgggctatg tgtatccgcc
541ttctatccag ccggcaccct atgctctaca gcttccagac atctctgccc aagcttcctg
601tgcccagggt gtcagccaca attcagcggt acctagagtc tgtgcgcccc ttgttggatg
661atgaggaata ttaccgcatg gagttgctgg ccaaagaatt ccaggacaag actgccccca
721ggctgcagaa atacctggtg ctcaagtcat ggtgggcaag taactatgtg agtgactggt
781gggaagagta catctacctt cgaggcagga gccctctcat ggtgaacagc aactattatg
841tcatggacct tgtgctcatc aagaatacag acgtgcaggc agcccgcctg ggaaacatca
901tccacgccat gatcatgtat cgccgtaaac tggaccgtga agaaatcaag cctgtgatgg
961cactgggcat agtgcctatg tgctcctacc agatggaggg gatgttcaac accactcgga
1021tcccgggcaa ggacacagat gtgctacagc acctctcaga cagccggcac gtggctgtct
1081accacaaggg acgcttcttc aagctgtggc tctatgaggg cgcccgtctg ctcaagcctc
1141aggatctgga gatgcagttc cagaggatcc tggacgaccc ctccccacct cagcctgggg
1201aggagaagct ggcagccctc actgcaggag gaagggtgga gtgggcgcag gcacgccagg
1261ccttctttag ctctggaaag aataaggctg ccttggaggc catcgagcgt gccgctttct
1321tcgtggccct ggatgaggaa tcctactcct atgaccccga agatgaggcc agcctcagcc
1381tctatggcaa ggccctgcta catggcaact gctacaacag gtggtttgac aaatccttca
1441ctctcatttc cttcaagaat ggccagttgg gtctcaatgc agagcatgcg tgggcagatg
1501ctcccatcat tgggcacctc tgggagtttg tcctgggcac agacagcttc cacctgggct
1561acacggagac cgggcactgc ctgggcaaac cgaaccctgc gctcgcacct cctacacggc
1621tgcagtggga cattccaaaa cagtgccagg cggtcatcga gagttcctac caggtggcca
1681aggcgttggc agacgacgtg gagttgtact gcttccagtt cctgcccttt ggcaaaggcc
1741tcatcaagaa gtgccggacc agccctgatg cctttgtgca gatcgcgctg cagctggctc
1801acttccggga caggggtaag ttctgcctga cctatgaggc ctcaatgacc agaatgttcc
1861gggagggacg gactgagact gtgcgttcct gtaccagcga gtccacagcc tttgtgcagg
1921ccatgatgga ggggtcccac acaaaagcag acctgcgaga tctcttccag aaggctgcta
1981agaagcacca gaatatgtac cgcctggcca tgaccggggc agggatcgac aggcacctct
2041tctgccttta cttggtctcc aagtacctag gagtcagctc tcctttcctt gctgaggtgc
2101tctcggaacc ctGGCGTCTC TCCACCAGCC AGATCCCCCA ATCCCAGATC CGCATGTTCG
2161ACCCAGAGCA GCACCCCAAT CACCTGGGCG CTGGAGGTGG CTTTGGCCCT GTAGCAGATG
2221ATGGCTATGG AGTTTCCTAC ATGATTGCAG GCGAGAACAC GATCTTCTTC CACATCTCCA
2281GCAAGTTCTC AAGCTCAGAG ACGAACGCCC AGCGCTTTGG AAACCACATC CGCAAAGCCC
2341TGCTGGACAT TGCTGATCTT TTCCAAGTTC CCAAGGCCTA CAGCTTGCCA ACTTTCTTGT
2401ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
2461AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
2521GAAAGGACGA CATCCACTGC TGTACAACGA GACCACGCGT TAAGTCgaca atcaacctct
2581ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)