Construct: ORF TRCN0000474369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014061.1_s317c1
- Derived from:
- ccsbBroadEn_06034
- DNA Barcode:
- CATCCACTGCTGTACAACGAGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPT1B (1375)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1375 | CPT1B | carnitine palmitoyltransfer... | NM_001145135.2 | 99.9% | 99.8% | 931A>G |
| 2 | human | 1375 | CPT1B | carnitine palmitoyltransfer... | NM_001145137.2 | 99.9% | 99.8% | 931A>G |
| 3 | human | 1375 | CPT1B | carnitine palmitoyltransfer... | NM_004377.4 | 99.9% | 99.8% | 931A>G |
| 4 | human | 1375 | CPT1B | carnitine palmitoyltransfer... | NM_152245.3 | 99.9% | 99.8% | 931A>G |
| 5 | human | 1375 | CPT1B | carnitine palmitoyltransfer... | NM_152246.3 | 99.9% | 99.8% | 931A>G |
| 6 | human | 1375 | CPT1B | carnitine palmitoyltransfer... | NM_001145134.2 | 95.5% | 95.4% | 459_460ins102;829A>G |
| 7 | human | 386593 | CHKB-CPT1B | CHKB-CPT1B readthrough (NMD... | NR_027928.2 | 53.7% | (many diffs) | |
| 8 | mouse | 12895 | Cpt1b | carnitine palmitoyltransfer... | NM_009948.2 | 85.8% | 86.9% | (many diffs) |
| 9 | mouse | 100134861 | Gm44502 | predicted readthrough trans... | NR_004843.2 | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2385
- ORF length:
- 2316
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaagct caccaggccg tggccttcca gttcacggtg accccagacg 121 gggtcgactt ccggctcagt cgggaggccc tgaaacacgt ctacctgtct gggatcaact 181 cctggaagaa acgcctgatc cgcatcaaga atggcatcct caggggcgtg taccctggca 241 gccccaccag ctggctggtc gtcatcatgg caacagtggg ttcctccttc tgcaacgtgg 301 acatctcctt ggggctggtc agttgcatcc agagatgcct ccctcagggg tgtggcccct 361 accagacccc gcagacccgg gcacttctca gcatggccat cttctccacg ggcgtctggg 421 tgacgggcat cttcttcttc cgccaaaccc tgaagctgct tctctgctac catgggtgga 481 tgtttgagat gcatggcaag accagcaact tgaccaggat ctgggctatg tgtatccgcc 541 ttctatccag ccggcaccct atgctctaca gcttccagac atctctgccc aagcttcctg 601 tgcccagggt gtcagccaca attcagcggt acctagagtc tgtgcgcccc ttgttggatg 661 atgaggaata ttaccgcatg gagttgctgg ccaaagaatt ccaggacaag actgccccca 721 ggctgcagaa atacctggtg ctcaagtcat ggtgggcaag taactatgtg agtgactggt 781 gggaagagta catctacctt cgaggcagga gccctctcat ggtgaacagc aactattatg 841 tcatggacct tgtgctcatc aagaatacag acgtgcaggc agcccgcctg ggaaacatca 901 tccacgccat gatcatgtat cgccgtaaac tggaccgtga agaaatcaag cctgtgatgg 961 cactgggcat agtgcctatg tgctcctacc agatggaggg gatgttcaac accactcgga 1021 tcccgggcaa ggacacagat gtgctacagc acctctcaga cagccggcac gtggctgtct 1081 accacaaggg acgcttcttc aagctgtggc tctatgaggg cgcccgtctg ctcaagcctc 1141 aggatctgga gatgcagttc cagaggatcc tggacgaccc ctccccacct cagcctgggg 1201 aggagaagct ggcagccctc actgcaggag gaagggtgga gtgggcgcag gcacgccagg 1261 ccttctttag ctctggaaag aataaggctg ccttggaggc catcgagcgt gccgctttct 1321 tcgtggccct ggatgaggaa tcctactcct atgaccccga agatgaggcc agcctcagcc 1381 tctatggcaa ggccctgcta catggcaact gctacaacag gtggtttgac aaatccttca 1441 ctctcatttc cttcaagaat ggccagttgg gtctcaatgc agagcatgcg tgggcagatg 1501 ctcccatcat tgggcacctc tgggagtttg tcctgggcac agacagcttc cacctgggct 1561 acacggagac cgggcactgc ctgggcaaac cgaaccctgc gctcgcacct cctacacggc 1621 tgcagtggga cattccaaaa cagtgccagg cggtcatcga gagttcctac caggtggcca 1681 aggcgttggc agacgacgtg gagttgtact gcttccagtt cctgcccttt ggcaaaggcc 1741 tcatcaagaa gtgccggacc agccctgatg cctttgtgca gatcgcgctg cagctggctc 1801 acttccggga caggggtaag ttctgcctga cctatgaggc ctcaatgacc agaatgttcc 1861 gggagggacg gactgagact gtgcgttcct gtaccagcga gtccacagcc tttgtgcagg 1921 ccatgatgga ggggtcccac acaaaagcag acctgcgaga tctcttccag aaggctgcta 1981 agaagcacca gaatatgtac cgcctggcca tgaccggggc agggatcgac aggcacctct 2041 tctgccttta cttggtctcc aagtacctag gagtcagctc tcctttcctt gctgaggtgc 2101 tctcggaacc ctGGCGTCTC TCCACCAGCC AGATCCCCCA ATCCCAGATC CGCATGTTCG 2161 ACCCAGAGCA GCACCCCAAT CACCTGGGCG CTGGAGGTGG CTTTGGCCCT GTAGCAGATG 2221 ATGGCTATGG AGTTTCCTAC ATGATTGCAG GCGAGAACAC GATCTTCTTC CACATCTCCA 2281 GCAAGTTCTC AAGCTCAGAG ACGAACGCCC AGCGCTTTGG AAACCACATC CGCAAAGCCC 2341 TGCTGGACAT TGCTGATCTT TTCCAAGTTC CCAAGGCCTA CAGCTTGCCA ACTTTCTTGT 2401 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2461 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2521 GAAAGGACGA CATCCACTGC TGTACAACGA GACCACGCGT TAAGTCgaca atcaacctct 2581 ggattacaaa atttgtgaaa gatt