Construct: ORF TRCN0000474389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016114.1_s317c1
- Derived from:
- ccsbBroadEn_06507
- DNA Barcode:
- CTCCAGCCACTGTTTCGGCTACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT18 (3875)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3875 | KRT18 | keratin 18 | NM_000224.3 | 99.8% | 99.5% | 5G>C;14C>T |
| 2 | human | 3875 | KRT18 | keratin 18 | NM_199187.1 | 99.8% | 99.5% | 5G>C;14C>T |
| 3 | human | 284085 | KRT18P55 | keratin 18 pseudogene 55 | NR_028334.1 | 59.6% | (many diffs) | |
| 4 | human | 693207 | MIR622 | microRNA 622 | NR_030754.1 | 7% | (many diffs) | |
| 5 | mouse | 16668 | Krt18 | keratin 18 | NM_010664.2 | 82.9% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1356
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cttcaccatt cgctccacct tctccaccaa ctaccggtcc ctgggctctg 121 tccaggcgcc cagctacggc gcccggccgg tcagcagcgc ggccagcgtc tatgcaggcg 181 ctgggggctc tggttcccgg atctccgtgt cccgctccac cagcttcagg ggcggcatgg 241 ggtccggggg cctggccacc gggatagccg ggggtctggc aggaatggga ggcatccaga 301 acgagaagga gaccatgcaa agcctgaacg accgcctggc ctcttacctg gacagagtga 361 ggagcctgga gaccgagaac cggaggctgg agagcaaaat ccgggagcac ttggagaaga 421 agggacccca ggtcagagac tggagccatt acttcaagat catcgaggac ctgagggctc 481 agatcttcgc aaatactgtg gacaatgccc gcatcgttct gcagattgac aatgcccgtc 541 ttgctgctga tgactttaga gtcaagtatg agacagagct ggccatgcgc cagtctgtgg 601 agaacgacat ccatgggctc cgcaaggtca ttgatgacac caatatcaca cgactgcagc 661 tggagacaga gatcgaggct ctcaaggagg agctgctctt catgaagaag aaccacgaag 721 aggaagtaaa aggcctacaa gcccagattg ccagctctgg gttgaccgtg gaggtagatg 781 cccccaaatc tcaggacctc gccaagatca tggcagacat ccGGGCCCAA TATGACGAGC 841 TGGCTCGGAA GAACCGAGAG GAGCTAGACA AGTACTGGTC TCAGCAGATT GAGGAGAGCA 901 CCACAGTGGT CACCACACAG TCTGCTGAGG TTGGAGCTGC TGAGACGACG CTCACAGAGC 961 TGAGACGTAC AGTCCAGTCC TTGGAGATCG ACCTGGACTC CATGAGAAAT CTGAAGGCCA 1021 GCTTGGAGAA CAGCCTGAGG GAGGTGGAGG CCCGCTACGC CCTACAGATG GAGCAGCTCA 1081 ACGGGATCCT GCTGCACCTT GAGTCAGAGC TGGCACAGAC CCGGGCAGAG GGACAGCGCC 1141 AGGCCCAGGA GTATGAGGCC CTGCTGAACA TCAAGGTCAA GCTGGAGGCT GAGATCGCCA 1201 CCTACCGCCG CCTGCTGGAA GATGGCGAGG ACTTTAATCT TGGTGATGCC TTGGACAGCA 1261 GCAACTCCAT GCAAACCATC CAAAAGACCA CCACCCGCCG GATAGTGGAT GGCAAAGTGG 1321 TGTCTGAGAC CAATGACACC AAAGTTCTGA GGCATTACCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1501 ACTCCAGCCA CTGTTTCGGC TACTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1561 aatttgtgaa agatt