Transcript: Human NM_199187.1

Homo sapiens keratin 18 (KRT18), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-23
Taxon:
Homo sapiens (human)
Gene:
KRT18 (3875)
Length:
1439
CDS:
70..1362

Additional Resources:

NCBI RefSeq record:
NM_199187.1
NBCI Gene record:
KRT18 (3875)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_199187.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000062365 GATGACACCAATATCACACGA pLKO.1 637 CDS 100% 2.640 2.112 N KRT18 n/a
2 TRCN0000299482 GATGACACCAATATCACACGA pLKO_005 637 CDS 100% 2.640 2.112 N KRT18 n/a
3 TRCN0000062367 GAGGACTTTAATCTTGGTGAT pLKO.1 1231 CDS 100% 4.050 2.835 N KRT18 n/a
4 TRCN0000062366 CTTCATGAAGAAGAACCACGA pLKO.1 702 CDS 100% 2.160 1.296 N KRT18 n/a
5 TRCN0000299480 CTTCATGAAGAAGAACCACGA pLKO_005 702 CDS 100% 2.160 1.296 N KRT18 n/a
6 TRCN0000295655 AGTACTGGTCTCAGCAGATTG pLKO_005 875 CDS 100% 10.800 5.400 Y Krt18 n/a
7 TRCN0000062363 CAGATTGACAATGCCCGTCTT pLKO.1 526 CDS 100% 4.050 2.025 Y KRT18 n/a
8 TRCN0000331652 CAGATTGACAATGCCCGTCTT pLKO_005 526 CDS 100% 4.050 2.025 Y KRT18 n/a
9 TRCN0000062364 CCTGCTGAACATCAAGGTCAA pLKO.1 1164 CDS 100% 4.050 2.025 Y KRT18 n/a
10 TRCN0000299481 CCTGCTGAACATCAAGGTCAA pLKO_005 1164 CDS 100% 4.050 2.025 Y KRT18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_199187.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487775 GTTCAACGCCCGGGTGCCGGACCC pLX_317 21.2% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_06508 pDONR223 100% 99.9% 99.7% None 502T>C n/a
3 ccsbBroad304_06508 pLX_304 0% 99.9% 99.7% V5 502T>C n/a
4 TRCN0000478565 GGTCTGGTAAACGCTAGTGTGTCC pLX_317 22.6% 99.9% 99.7% V5 502T>C n/a
5 ccsbBroadEn_06507 pDONR223 100% 99.8% 99.5% None 5G>C;14C>T n/a
6 ccsbBroad304_06507 pLX_304 0% 99.8% 99.5% V5 5G>C;14C>T n/a
7 TRCN0000474389 CTCCAGCCACTGTTTCGGCTACTC pLX_317 41.5% 99.8% 99.5% V5 5G>C;14C>T n/a
8 ccsbBroadEn_10600 pDONR223 100% 42.6% 35.3% None (many diffs) n/a
9 ccsbBroad304_10600 pLX_304 0% 42.6% 35.3% V5 (many diffs) n/a
10 TRCN0000475856 AAAGGATTTTAGCATTTTCCACAA pLX_317 34.6% 42.6% 35.3% V5 (many diffs) n/a
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