Construct: ORF TRCN0000474395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018884.3_s317c1
- Derived from:
- ccsbBroadEn_14951
- DNA Barcode:
- GCCAGAGTCTAAGCCCTCGGCCAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GNE (10020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474395
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_005476.7 | 99.4% | 33.7% | (many diffs) |
| 2 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | XM_017014167.1 | 99.4% | 33.7% | (many diffs) |
| 3 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001128227.3 | 95.3% | 32.4% | (many diffs) |
| 4 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001190388.1 | 93.4% | 27.1% | (many diffs) |
| 5 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | XM_017014168.1 | 92.4% | 31.4% | (many diffs) |
| 6 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001190383.3 | 89.6% | 37.6% | 733delA;1374A>C;1409_1410ins222 |
| 7 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | XM_005251334.4 | 88.6% | 30% | (many diffs) |
| 8 | human | 10020 | GNE | glucosamine (UDP-N-acetyl)-... | NM_001190384.3 | 84.2% | 22.7% | (many diffs) |
| 9 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | NM_001190414.1 | 87.1% | 33.5% | (many diffs) |
| 10 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | XM_006538073.3 | 84.2% | 32.3% | (many diffs) |
| 11 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | NM_015828.3 | 83.5% | 32.1% | (many diffs) |
| 12 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | XM_006538074.3 | 80.5% | 25.4% | (many diffs) |
| 13 | mouse | 50798 | Gne | glucosamine (UDP-N-acetyl)-... | XM_017320310.1 | 62.7% | 5.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 810
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaagaat ggaaataacc gaaagctgcg ggtttgtgtt gctacttgta 121 accgtgcaga ttattctaaa cttgccccga tcatgtttgg cattaaaacc gaacctgagt 181 tctttgaact tgatgttgtg gtacttggct ctcacctgat agatgactat ggaaatacat 241 atcgaatgat tgaacaagat gactttgaca ttaacaccag gctacacaca attgtgaggg 301 gagaagatga ggcagccatg gtggagtcag taggcctggc cctagtgaag ctgccagatg 361 tccttaatcg cctgaagcct gatatcatga ttgttcatgg agacaggttt gatgccctgg 421 ctctggccac atctgctgcc ttgatgaaca tccgaatcct tcacattgaa ggtggggaag 481 tcagtgggac cattgatgac tctatcagac atgccataac aaaactggct cattatcatg 541 tgtgctgcac ccgcagtgca gagcagcacc tgatatccat gtgtgaggac catgatcgca 601 tccttttggc aggctgccct tcctatgaca aacttctctc agccaagaac aaagactaca 661 tgagcatcat tcgcatgtgg ctaggtgatg atgtaaaatc taaagattac attgttgcac 721 tacagcaccc tgtgaccact gacattaagc attccataaa aatgtttgaa ttaacattgg 781 atgcacttat ctcatttaac agcggaccct agtcctgttt ccaaatattg acgcagggag 841 caaagagatg gttcgagtga tgcggaagaa gggcattgag catcatccca actttcgtgc 901 agttaaacac gtcccatttg accagtttat acagttggtt gcccatgctg gctgtatgat 961 tgggaacagc agctgtgggg ttcgagaagt tggagctttt ggaacacctg tgatcaacct 1021 gggaacacgt cagattggaa gagaaacagg ggagaatgtt cttcatgtcc gggatgctga 1081 cacccaagac aaaatattgc aagcactgca ccttcagttt ggtaaacagt acccttgttc 1141 aaagatatat ggggatggaa atgctgttcc aaggattttg aagtttctca aatctatcga 1201 tcttcaagag ccactgcaaa agaaattctg ctttcctcct gtgaaggaga atatctctca 1261 agatattgac catattcttg aaactctaag tgccttggcc gttgatcttg gcgggacgaa 1321 cctccgagtt gcaatagtca gcatgaaggg tgaaatagtt aagaagtata ctcagttcaa 1381 tcctaaaacc tatgaagaga ggattaattt aatcctacag atgtgtgtgg aagctgcagc 1441 cgaagctgta aaactgaact gcagaatttt gggagtaggc atttccacag gtgnccgtgt 1501 aatcttcgga aaaggaaatg tgctgcattc aacccaactg atccaagagt ggaactctgt 1561 gacntttagg accccccttt ctgacacttt gcatctccct gtgtgggtag acaatggtgg 1621 caactgtgct gccctggcgg aaaggaaatt tggccaagga aagggactgg aaaactttgt 1681 tacacttatc acaggcacag gaatcggtgg tggaattatc catcagcatg aattgatcca 1741 cggaagctcc ttctgtgctg cagaactggg ccaccttgtt gtgtctctgg atgggcctga 1801 ttgttcctgt ggaagccatg ggtgcattga agcatacgcc tctggaatgg ccTTGCAGAG 1861 GGAGGCAAAA AAGCTCCATG ATGAGGACCT GCTCTTGGTG GAAGGGATGT CAGTGCCAAA 1921 AGATGAGGCT GTGGGTGCGC TCCATCTCAT CCAAGCTGCG AAACTTGGCA ATGCGAAGGC 1981 CCAGAGCATC CTAAGAACAG CTGGAACAGC TTTGGGTCTT GGGGTTGTGA ACATCCTCCA 2041 TACCATGAAT CCCTCCCTTG TGATCCTCTC CGGAGTCCTG GCCAGTCACT ATATCCACAT 2101 TGTCAAAGAC GTCATTCGCC AGCAGGCCTT GTCCTCCGTG CAGGACGTGG ATGTGGTGGT 2161 TTCGGATTTG GTTGACCCCG CCCTGCTGGG TGCTGCCAGC ATGGTTCTGG ACTACACAAC 2221 ACGCAGGATC TACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 2281 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 2341 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CCAGAGTCTA AGCCCTCGGC 2401 CAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att