Construct: ORF TRCN0000474405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006744.1_s317c1
- Derived from:
- ccsbBroadEn_04605
- DNA Barcode:
- TTACTCACTTTATGACCACAACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRFAP1 (93621)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_001272053.1 | 100% | 100% | |
| 2 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_033296.2 | 100% | 100% | |
| 3 | human | 114932 | MRFAP1L1 | Morf4 family associated pro... | NM_203462.3 | 88% | 85% | (many diffs) |
| 4 | human | 114932 | MRFAP1L1 | Morf4 family associated pro... | XM_005247932.2 | 88% | 85% | (many diffs) |
| 5 | human | 93621 | MRFAP1 | Morf4 family associated pro... | NM_001272054.1 | 81.1% | 78.7% | 291_292ins72 |
| 6 | mouse | 67568 | Mrfap1 | Morf4 family associated pro... | NM_026242.3 | 84.3% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 471
- ORF length:
- 381
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCGGCCCCT GGACATCGTC GAGCTGGCGG 121 AACCGGAGGA AGTGGAGGTG CTGGAGCCCG AGGAGGATTT CGAGCAGTTT CTGCTCCCGG 181 TCATCAACGA GATGCGCGAG GACATCGCGT CGCTGACGCG CGAGCACGGG CGGGCGTACC 241 TGCGGAACCG GAGCAAGCTG TGGGAGATGG ACAATATGCT CATCCAGATC AAAACGCAGG 301 TGGAGGCCTC GGAGGAGAGC GCCCTCAACC ACCTCCAGAA CCCGGGCGAC GCGGCCGAGG 361 GCCGGGCGGC CAAGAGGTGC GAGAAGGCCG AGGAGAAGGC CAAGGAGATT GCGAAGATGG 421 CAGAGATGCT GGTGGAGCTG GTCCGGCGGA TAGAGAAGAG CGAGTCGTCG TGCCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGATTAC TCACTTTATG ACCACAACAA ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt