Construct: ORF TRCN0000474424
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003003.1_s317c1
- Derived from:
- ccsbBroadEn_07201
- DNA Barcode:
- CCCCCCTTTGCCAATTTCCAATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALX1 (8092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474424
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8092 | ALX1 | ALX homeobox 1 | NM_006982.3 | 99.8% | 99.6% | 963T>G |
| 2 | human | 8092 | ALX1 | ALX homeobox 1 | XM_011538782.2 | 70.7% | 70.5% | 0_1ins285;678T>G |
| 3 | mouse | 216285 | Alx1 | ALX homeobox 1 | NM_172553.4 | 90.6% | 96.9% | (many diffs) |
| 4 | mouse | 216285 | Alx1 | ALX homeobox 1 | XM_006513529.2 | 90.6% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaacg 61 ttggcatgga gtttctgagc gagaagtttg ccctcaagag ccctccgagt aaaaacagtg 121 acttttacat gggcgcagga ggtcctctgg agcacgttat ggagacgctg gacaatgagt 181 ccttttacag caaagcgtct gcaggcaaat gcgtgcaggc cttcggaccc ctgccccgcg 241 ccgagcatca cgtgcgcttg gagaggacct cgccctgtca ggacagcagc gtgaactatg 301 ggatcactaa agtagaagga cagccccttc acaccgaact gaatagagct atggacaact 361 gtaacagtct ccgaatgtct cccgtgaaag ggatgcaaga gaagggagag ctggatgaac 421 ttggggataa atgtgatagc aatgtatcca gcagtaagaa acggaggcac cgaaccacct 481 tcaccagttt gcagctagag gagctggaga aagtctttca gaaaactcat tacccggatg 541 tgtatgtcag agaacagctt gctctgagga cagagcTCAC TGAGGCCAGG GTCCAGGTTT 601 GGTTTCAAAA TCGAAGGGCC AAATGGAGAA AAAGGGAACG TTATGGCCAA ATACAACAAG 661 CGAAAAGCCA TTTTGCTGCC ACCTATGATA TATCAGTTTT GCCAAGGACT GACAGCTACC 721 CACAGATTCA GAACAATTTG TGGGCAGGAA ATGCAAGTGG TGGTTCTGTG GTTACTTCAT 781 GCATGTTACC ACGTGACACT TCCTCCTGTA TGACACCTTA TTCTCACTCG CCTCGGACAG 841 ATTCCAGTTA CACGGGGTTT TCAAACCACC AGAACCAGTT CAGCCACGTG CCCCTCAACA 901 ATTTTTTCAC TGACTCTCTT CTTACTGGGG CAACCAATGG ACATGCATTT GAAACAAAGC 961 CAGAGTTTGA AAGGAGGTCT TCCAGTATCG CAGTTCTTCG AATGAAAGCC AAGGAGCACA 1021 CCGCCAAGAT TTCATGGGCC ATGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CCCCCTTTGC 1201 CAATTTCCAA TTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att