Transcript: Human NM_006982.3

Homo sapiens ALX homeobox 1 (ALX1), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
ALX1 (8092)
Length:
1332
CDS:
43..1023

Additional Resources:

NCBI RefSeq record:
NM_006982.3
NBCI Gene record:
ALX1 (8092)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006982.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418232 AGGAGCACACCGCCAATATTT pLKO_005 989 CDS 100% 15.000 21.000 N Alx1 n/a
2 TRCN0000013149 GCGTGAACTATGGGATCACTA pLKO.1 266 CDS 100% 4.950 6.930 N ALX1 n/a
3 TRCN0000013151 CGCCAATATTTCATGGGCCAT pLKO.1 999 CDS 100% 2.160 3.024 N ALX1 n/a
4 TRCN0000421889 CTCCTGTATGACACCTTATTC pLKO_005 780 CDS 100% 13.200 9.240 N ALX1 n/a
5 TRCN0000416541 AGGTCCTCTGGAGCACGTTAT pLKO_005 117 CDS 100% 10.800 7.560 N ALX1 n/a
6 TRCN0000427453 ATGTATCCAGCAGTAAGAAAC pLKO_005 419 CDS 100% 10.800 7.560 N ALX1 n/a
7 TRCN0000013148 GAATTGCTAAAGGTCAAGATA pLKO.1 1116 3UTR 100% 5.625 3.938 N ALX1 n/a
8 TRCN0000013152 CCACCTATGATATATCAGTTT pLKO.1 656 CDS 100% 4.950 3.465 N ALX1 n/a
9 TRCN0000013150 CCGGATGTGTATGTCAGAGAA pLKO.1 511 CDS 100% 4.950 3.465 N ALX1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006982.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07201 pDONR223 100% 99.8% 99.6% None 963T>G n/a
2 ccsbBroad304_07201 pLX_304 0% 99.8% 99.6% V5 963T>G n/a
3 TRCN0000474424 CCCCCCTTTGCCAATTTCCAATTA pLX_317 47.8% 99.8% 99.6% V5 963T>G n/a
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