Construct: ORF TRCN0000474432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004969.1_s317c1
- Derived from:
- ccsbBroadEn_08236
- DNA Barcode:
- AAATTCCAGCTATTAGCTTCGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EGFL7 (51162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51162 | EGFL7 | EGF like domain multiple 7 | NM_016215.5 | 99.8% | 99.6% | 457G>A |
| 2 | human | 51162 | EGFL7 | EGF like domain multiple 7 | NM_201446.3 | 99.8% | 99.6% | 457G>A |
| 3 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_011518764.1 | 99.8% | 99.6% | 457G>A |
| 4 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_011518765.1 | 99.8% | 99.6% | 457G>A |
| 5 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_011518766.1 | 99.8% | 99.6% | 457G>A |
| 6 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_006717141.3 | 80.2% | 80.2% | 406_407ins162 |
| 7 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_017014797.1 | 80.2% | 80.2% | 406_407ins162 |
| 8 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_011518767.1 | 78.5% | 61.6% | (many diffs) |
| 9 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_011518768.1 | 78.5% | 61.6% | (many diffs) |
| 10 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_017014798.1 | 78.5% | 61.6% | (many diffs) |
| 11 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_017014799.1 | 78.5% | 61.6% | (many diffs) |
| 12 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_017014795.1 | 77.3% | 65.3% | (many diffs) |
| 13 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XM_017014796.1 | 76% | 65.9% | (many diffs) |
| 14 | human | 51162 | EGFL7 | EGF like domain multiple 7 | NR_045110.2 | 53.1% | 1_326del;783G>A;1146_1540del | |
| 15 | human | 51162 | EGFL7 | EGF like domain multiple 7 | NR_045111.2 | 53% | 1_329del;786G>A;1149_1543del | |
| 16 | human | 51162 | EGFL7 | EGF like domain multiple 7 | NR_046367.1 | 38.2% | 1_911del;1368G>A;1731_2141del | |
| 17 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XR_002956791.1 | 34.1% | (many diffs) | |
| 18 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XR_002956790.1 | 12.9% | (many diffs) | |
| 19 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XR_002956789.1 | 12.3% | (many diffs) | |
| 20 | human | 51162 | EGFL7 | EGF like domain multiple 7 | XR_001746317.1 | 6.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gggctctcag gaggtgctgc tgatgtggct tctggtgttg gcagtgggcg 121 gcacagagca cgcctaccgg cccggccgta gggtgtgtgc tgtccgggct cacggggacc 181 ctgtctccga gtcgttcgtg cagcgtgtgt accagccctt cctcaccacc tgcgacgggc 241 accgggcctg cagcacctac cgaaccatct ataggaccgc ctaccgccgc agccctgggc 301 tggcccctgc caggcctcgc tacgcgtgct gccccggctg gaagaggacc agcgggcttc 361 ctggggcctg tggagcagca atatgccagc cgccatgccg gaacggaggg agctgtgtcc 421 agcctggccg ctgccgctgc cctgcaggat ggcggggtga cacttgccag tcagatgtgg 481 atgaatGCAG TGCTAGGAGG GGCGGCTGTC CCCAGCGCTG CATCAACACC GCCGGCAGTT 541 ACTGGTGCCA GTGTTGGGAG GGGCACAGCC TGTCTGCAGA CGGTACACTC TGTGTGCCCA 601 AGGGAGGGCC CCCCAGGGTG GCCCCCAACC CGACAGGAGT GGACAGTGCA ATGAAGGAAG 661 AAGTGCAGAG GCTGCAGTCC AGGGTGGACC TGCTGGAGGA GAAGCTGCAG CTGGTGCTGG 721 CCCCACTGCA CAGCCTGGCC TCGCAGGCAC TGGAGCATGG GCTCCCGGAC CCCGGCAGCC 781 TCCTGGTGCA CTCCTTCCAG CAGCTCGGCC GCATCGACTC CCTGAGCGAG CAGATTTCCT 841 TCCTGGAGGA GCAGCTGGGG TCCTGCTCCT GCAAGAAAGA CTCGTGCCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA AAATTCCAGC TATTAGCTTC GAGTACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt