Construct: ORF TRCN0000474493
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008546.1_s317c1
- Derived from:
- ccsbBroadEn_10452
- DNA Barcode:
- GTTTCGCGACTGAAGTTATGTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAM2 (10254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474493
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10254 | STAM2 | signal transducing adaptor ... | NM_005843.6 | 99.8% | 99.6% | 1565A>C;1573C>A |
| 2 | human | 10254 | STAM2 | signal transducing adaptor ... | XR_001738586.1 | 25.8% | (many diffs) | |
| 3 | mouse | 56324 | Stam2 | signal transducing adaptor ... | NM_019667.2 | 85.7% | 86% | (many diffs) |
| 4 | mouse | 56324 | Stam2 | signal transducing adaptor ... | XM_006498198.3 | 80.8% | 80.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1641
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tttgttcacc gccaacccct tcgagcaaga cgtggaaaaa gccacgaatg 121 agtacaacac tacagaagat tggagtctta ttatggacat atgtgacaaa gttggaagta 181 ctcctaatgg agcgaaagat tgcctaaaag ccataatgaa aagggtaaat cataaggttc 241 cacatgttgc tctgcaagca ctaactcttc ttggggcttg tgtggcaaac tgtggaaaga 301 tatttcattt agaagtatgt tcccgtgatt ttgcaacaga agtacgtgct gtgattaaaa 361 ataaggcaca tcctaaagta tgtgaaaaac tgaaatcttt aatggtggag tggtcagaag 421 aatttcagaa ggaccctcag tttagtctga tatctgcaac tattaaatct atgaaagaag 481 aaggaattac ttttcctcca gcaggttctc agactgtctc agctgctgcc aagaatggta 541 cgtcatcgaa caaaaacaaa gaggatgaag acatagctaa agctattgaa ttatcgctgc 601 aagaacagaa acagcaacac acagaaacaa aatccttata tccatcttca gaaattcagt 661 taaataataa ggttgcacgg aaagtgagag ctttatatga ttttgaagct gttgaggaca 721 atgaactcac ctttaaacat ggtgaaataa ttattgtttt ggatgacagt gatgccaatt 781 ggtggaaagg agaaaatcac agaggaatag gacttttccc atccaatttt gtaacaacta 841 atttaaacat agagactgag gcagcggctg tggacaaatt gaatgtaatt gatgatgatg 901 tggaggaaat taagaaatca gagcctgagc ctgtttatat agatgaggat aagatggata 961 gagccctgca ggtacttcag agtatagatc caacagattc aaaaccagac tcccaagacc 1021 ttttggattt agaagatatc tgccaacaga tgggtccaat gatagatgaa aaacttgaag 1081 aaattgatag gaagcattca gaattgtctg aattgaatgt taaagtcctg gaagctctgg 1141 aactatataa caaattggtg aatgaagcac cagtgtactc agtctattca aagctccacc 1201 ctccagcaca ttacccacct gcatcatctg gggttcCAAT GCAGACATAT CCAGTTCAAT 1261 CACATGGTGG AAACTATATG GGTCAGAGCA TTCACCAAGT AACTGTTGCC CAAAGCTATA 1321 GCCTAGGACC CGATCAAATT GGTCCACTGA GATCTCTGCC TCCAAATGTG AATTCCTCAG 1381 TGACAGCACA GCCTGCTCAA ACTTCATATT TAAGCACTGG ACAAGACACT GTTTCCAATC 1441 CTACTTATAT GAACCAGAAC TCTAACCTAC AGTCAGCTAC TGGTACAACT GCTTACACAC 1501 AGCAAATGGG GATGTCTGTG GATATGTCAT CTTATCAGAA CACTACTTCC AATTTGCCTC 1561 AACTGGCAGG CTTTCCGGTG ACAGTTCCAG CTCATCCAGT TGCACAGCAG CACACAAATT 1621 ACCATCAGCC GCCTCTCATT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTTT CGCGACTGAA 1801 GTTATGTAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt