Transcript: Mouse XM_006498198.3

PREDICTED: Mus musculus signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 (Stam2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Stam2 (56324)
Length:
3314
CDS:
133..1617

Additional Resources:

NCBI RefSeq record:
XM_006498198.3
NBCI Gene record:
Stam2 (56324)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006498198.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000065579 CGGAAGTACGTTCTGTGATAA pLKO.1 317 CDS 100% 13.200 18.480 N Stam2 n/a
2 TRCN0000065581 GTCAGAGCTTTATACGACTTT pLKO.1 664 CDS 100% 4.950 6.930 N Stam2 n/a
3 TRCN0000326630 GTCAGAGCTTTATACGACTTT pLKO_005 664 CDS 100% 4.950 6.930 N Stam2 n/a
4 TRCN0000306339 AGTCTGATATCTGCAACTATT pLKO_005 424 CDS 100% 13.200 10.560 N Stam2 n/a
5 TRCN0000065582 GCGCCTGTGTACTCGGTCTAT pLKO.1 1147 CDS 100% 1.650 1.320 N Stam2 n/a
6 TRCN0000326705 GCGCCTGTGTACTCGGTCTAT pLKO_005 1147 CDS 100% 1.650 1.320 N Stam2 n/a
7 TRCN0000306340 AGAAGTATGTTCCCGTGATTT pLKO_005 291 CDS 100% 13.200 9.240 N Stam2 n/a
8 TRCN0000065580 CCGTCCAACCTCCATACTTAA pLKO.1 1367 CDS 100% 13.200 9.240 N Stam2 n/a
9 TRCN0000306338 TTTAGCTTGCCAGCATATTTC pLKO_005 2062 3UTR 100% 13.200 9.240 N Stam2 n/a
10 TRCN0000065578 CGGTGGACAAATTGAATGTAA pLKO.1 848 CDS 100% 5.625 3.938 N Stam2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006498198.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10452 pDONR223 100% 80.8% 80.5% None (many diffs) n/a
2 ccsbBroad304_10452 pLX_304 0% 80.8% 80.5% V5 (many diffs) n/a
3 TRCN0000474493 GTTTCGCGACTGAAGTTATGTAAA pLX_317 26% 80.8% 80.5% V5 (many diffs) n/a
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