Construct: ORF TRCN0000474494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013376.1_s317c1
- Derived from:
- ccsbBroadEn_02777
- DNA Barcode:
- GCTGCTAACCAGGACTGGCGTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PES1 (23481)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23481 | PES1 | pescadillo ribosomal biogen... | NM_014303.4 | 100% | 100% | |
| 2 | human | 23481 | PES1 | pescadillo ribosomal biogen... | XM_006724173.2 | 99.8% | 99.8% | 1517_1518insGAA |
| 3 | human | 23481 | PES1 | pescadillo ribosomal biogen... | NM_001243225.2 | 99.1% | 99.1% | 911_912insGGAGATGTCAGCGCA |
| 4 | human | 23481 | PES1 | pescadillo ribosomal biogen... | NM_001282327.1 | 76.3% | 76.3% | 0_1ins417 |
| 5 | human | 23481 | PES1 | pescadillo ribosomal biogen... | NM_001282328.1 | 76.3% | 76.3% | 0_1ins417 |
| 6 | human | 23481 | PES1 | pescadillo ribosomal biogen... | XM_017028678.1 | 76.3% | 76.3% | 0_1ins417 |
| 7 | mouse | 64934 | Pes1 | pescadillo ribosomal biogen... | NM_022889.3 | 84.9% | 89.1% | (many diffs) |
| 8 | mouse | 64934 | Pes1 | pescadillo ribosomal biogen... | XM_017314702.1 | 82% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1830
- ORF length:
- 1764
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aggccttgag aagaagaagt atgaacgagg ctcggccacc aactacatca 121 cccggaacaa agcccggaag aagctccagc tgagcttggc tgactttagg cggctgtgca 181 ttctgaaggg catttatccc catgaaccca aacacaagaa gaaggttaac aagggttcta 241 cagcagcccg aacgttttac cttatcaaag acatcaggtt tctcctccac gaacccattg 301 tcaacaagtt ccgtgaatac aaggtgttcg tccggaagct ccggaaggct tatgggaaga 361 gcgagtggaa cactgtagag cgtttaaagg acaataagcc caactacaaa ctcgaccaca 421 tcatcaagga acggtatccc acgttcatcg atgccctgcg ggacctggac gatgccctct 481 ccatgtgctt cctgttttcc accttcccgc ggactggcaa gtgccacgtg cagaccattc 541 agctgtgccg ccggctcact gtggagttca tgcactacat tatcgctgcc cgtgccctgc 601 gcaaggtctt cctgtccatc aaaggcattt actaccaggc cgaggtactg gggcagccca 661 tcgtgtggat cactccctat gccttctccc atgaccaccc gacagacgtg gactacaggg 721 tcatggccac cttcaccgag ttctacacca cgctgctggg ctttgtcaac ttccgccttt 781 accagttgct caacctccac tatcccccga agctcgaggg tcaggcccaa gcagaggcaa 841 aggccggtga gggcacctac gcgttggact ccgagagttg tatggagaaa ctggcagccc 901 tcagtgccag cctggcccgc gtggtggtgc ctgccacaga ggaggaggcc gaggtggatg 961 agtttcccac cgatggggag atgtcagcgc aggaggaaga ccgcaggaag gagctggagg 1021 cgcaggagaa gcacaagaag ctttttgagg gcctgaagtt cttcctgaac cgagaggtgc 1081 cccgtgaggc cctggccttc atcatcagga gttttggtgg ggaagtgtcc tgggacaaat 1141 ctttgtgcat tggggccacc tatgacgtca cagactcccg catcacccat cagattgtcg 1201 accggcctgg gcagcagacc tcagtcattg gcaggtgcta cgtgcagccc cagtgggtgt 1261 ttgactcagt gaacgccagg ctccttctcc ccgtggcaga gtacttctct ggggtgcagc 1321 tgcccccaca cctttcaccc tttgtgaccg agaaggaagg agattacgtt ccacctgaga 1381 agctgaagct gctggctctg cagcggggag aggacccagg aaacctGAAT GAGTCAGAAG 1441 AGGAGGAGGA AGAGGACGAC AACAACGAAG GTGATGGTGA TGAAGAGGGA GAAAATGAGG 1501 AGGAGGAGGA AGATGCAGAG GCTGGTTCAG AAAAGGAGGA AGAGGCCCGG CTGGCAGCCC 1561 TGGAAGAGCA GAGGATGGAG GGGAAGAAGC CCAGGGTGAT GGCAGGCACC TTGAAGCTGG 1621 AGGATAAGCA GCGGCTGGCC CAGGAGGAGG AGAGTGAGGC CAAGCGCCTG GCCATTATGA 1681 TGATGAAGAA GCGGGAGAAG TACCTGTACC AGAAGATCAT GTTTGGCAAG AGGCGAAAAA 1741 TCCGAGAGGC CAACAAGCTG GCGGAGAAGC GGAAAGCCCA CGATGAGGCG GTGAGGTCTG 1801 AGAAGAAGGC CAAGAAGGCA AGGCCGGAGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1861 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1921 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCTGC 1981 TAACCAGGAC TGGCGTCTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2041 tgaaagatt