Construct: ORF TRCN0000474499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012467.1_s317c1
- Derived from:
- ccsbBroadEn_03977
- DNA Barcode:
- TGAGGTGAACGATCACGCCTAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNPNAT1 (64841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474499
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64841 | GNPNAT1 | glucosamine-phosphate N-ace... | NM_198066.4 | 100% | 100% | |
| 2 | human | 64841 | GNPNAT1 | glucosamine-phosphate N-ace... | XM_005268012.3 | 100% | 100% | |
| 3 | human | 64841 | GNPNAT1 | glucosamine-phosphate N-ace... | XM_006720238.3 | 100% | 100% | |
| 4 | mouse | 54342 | Gnpnat1 | glucosamine-phosphate N-ace... | NM_019425.2 | 91.6% | 98.3% | (many diffs) |
| 5 | mouse | 54342 | Gnpnat1 | glucosamine-phosphate N-ace... | XM_006519275.2 | 91.6% | 98.3% | (many diffs) |
| 6 | mouse | 54342 | Gnpnat1 | glucosamine-phosphate N-ace... | XM_006519276.3 | 91.6% | 98.3% | (many diffs) |
| 7 | mouse | 54342 | Gnpnat1 | glucosamine-phosphate N-ace... | XM_006519274.2 | 67.2% | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa acctgatgaa actcctatgt ttgacccaag tcTACTCAAA GAAGTGGACT 121 GGAGTCAGAA TACAGCTACA TTTTCTCCAG CCATTTCCCC AACACATCCT GGAGAAGGCT 181 TGGTTTTGAG GCCTCTTTGT ACTGCTGACT TAAATAGAGG TTTTTTTAAG GTATTGGGTC 241 AGCTAACAGA GACTGGAGTT GTCAGCCCTG AACAATTTAT GAAATCTTTT GAGCATATGA 301 AGAAATCTGG GGATTATTAT GTTACAGTTG TAGAAGATGT GACTCTAGGA CAGATTGTTG 361 CTACGGCAAC TCTGATTATA GAACATAAAT TCATCCATTC CTGTGCTAAG AGAGGAAGAG 421 TAGAAGATGT TGTTGTTAGT GATGAATGCA GAGGAAAGCA GCTTGGCAAA TTGTTATTAT 481 CAACCCTTAC TTTGCTAAGC AAGAAACTGA ACTGTTACAA GATTACCCTT GAATGTCTAC 541 CACAAAATGT TGGTTTCTAT AAAAAGTTTG GATATACTGT ATCTGAAGAA AACTACATGT 601 GTCGGAGGTT TCTAAAGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGAGGTG AACGATCACG 781 CCTAATTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt