Construct: ORF TRCN0000474500

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006232.1_s317c1
Derived from:
ccsbBroadEn_08137
DNA Barcode:
GTGCCTTTCCGCTCGCGTGGTTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HOOK2 (29911)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474500
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29911 HOOK2 hook microtubule tethering ... NM_001100176.2 99.9% 99.8% 1464C>G
2 human 29911 HOOK2 hook microtubule tethering ... XM_005259874.2 99.8% 99.7% 1464C>G;1717_1718insCAG
3 human 29911 HOOK2 hook microtubule tethering ... NM_013312.3 99.6% 99.5% 1464C>G;1599_1604delTGCCAT
4 human 29911 HOOK2 hook microtubule tethering ... XM_005259873.2 99.5% 99.4% 1464C>G;1599_1604delTGCCAT;1723_1724insCAG
5 human 29911 HOOK2 hook microtubule tethering ... XM_017026721.1 89.7% 89.6% 0_1ins219;1245C>G
6 human 29911 HOOK2 hook microtubule tethering ... XM_005259876.4 89.5% 89.4% 0_1ins219;1245C>G;1380_1385delTGCCAT
7 human 29911 HOOK2 hook microtubule tethering ... XM_005259877.4 89.5% 89.4% 0_1ins219;1245C>G;1380_1385delTGCCAT
8 human 29911 HOOK2 hook microtubule tethering ... XM_011527943.2 89.5% 89.4% 0_1ins219;1245C>G;1380_1385delTGCCAT
9 human 29911 HOOK2 hook microtubule tethering ... XM_011527944.2 89.5% 89.4% 0_1ins219;1245C>G;1380_1385delTGCCAT
10 human 29911 HOOK2 hook microtubule tethering ... XM_011527945.2 89.5% 89.4% 0_1ins219;1245C>G;1380_1385delTGCCAT
11 human 29911 HOOK2 hook microtubule tethering ... XM_011527946.2 89.5% 89.4% 0_1ins219;1245C>G;1380_1385delTGCCAT
12 human 29911 HOOK2 hook microtubule tethering ... XM_017026720.1 89.5% 89.4% 0_1ins219;1245C>G;1380_1385delTGCCAT
13 human 29911 HOOK2 hook microtubule tethering ... XM_011527947.2 80.8% 80.8% 0_1ins405;1059C>G;1194_1199delTGCCAT
14 mouse 170833 Hook2 hook microtubule tethering ... NM_133255.2 83.4% 86% (many diffs)
15 mouse 170833 Hook2 hook microtubule tethering ... NM_001167991.1 83.2% 85.9% (many diffs)
16 mouse 170833 Hook2 hook microtubule tethering ... XM_011248323.2 83.1% 85.6% (many diffs)
17 mouse 170833 Hook2 hook microtubule tethering ... XM_017312592.1 83% 85.6% (many diffs)
18 mouse 170833 Hook2 hook microtubule tethering ... XM_006530740.3 81.9% 84.4% (many diffs)
19 mouse 170833 Hook2 hook microtubule tethering ... XM_006530741.3 81.7% 84.2% (many diffs)
20 mouse 170833 Hook2 hook microtubule tethering ... XM_006530739.3 81.7% 84.1% (many diffs)
21 mouse 170833 Hook2 hook microtubule tethering ... XM_006530738.3 81.6% 84% (many diffs)
22 mouse 170833 Hook2 hook microtubule tethering ... XM_017312591.1 81.5% 84% (many diffs)
23 mouse 170833 Hook2 hook microtubule tethering ... XM_006530736.3 76.5% 78.7% (many diffs)
24 mouse 170833 Hook2 hook microtubule tethering ... XM_006530737.3 76.4% 78.6% (many diffs)
25 mouse 170833 Hook2 hook microtubule tethering ... XM_006530742.2 67.3% 70.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2217
ORF length:
2151
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag cgtggacaaa gctgagctat gcgggtctct gctcacctgg ttacagacgt
121tccacgttcc gtctccctgt gccagccctc aggacctgag cagcggcctt gccgtagcct
181atgtgctgaa ccagatagac ccctcctggt tcaacgaggc atggctccag ggcatctcgg
241aagatccagg tcccaactgg aagctgaagg tcagcaatct gaagatggtc ttacggagcc
301tagtagagta ctcccaggat gtcctggcgc atcctgtgtc agaagagcat ctcccagatg
361tgagcctcat tggagagttc tcagacccgg cagagctcgg caagctgctt cagctggtgc
421tgggctgtgc catcagttgc gagaaaaagc aggaccacat ccagagaatc atgacgctgg
481aagaatcggt tcagcatgtg gtgatggaag ccatccaaga gctcatgacc aaagacactc
541ctgactccct gtcaccagag acgtatggca actttgacag ccagtcccgc aggtactatt
601tcctaagtga ggaggctgag gagggggacg aattacagca gcgctgtctg gatctggagc
661ggcagctgat gctcctgtca gaggagaagc agagcctggc gcaagagaat gcagggctgc
721gggagcggat gggccggcct gaaggcgagg gtaccccagg tctcactgcc aagaagctgc
781tgctgctgca atcccagctg gagcagttgc aggaggagaa cttcaggctg gagagtggca
841gggaggatga gcgcctgcgc tgtgccgagc tggagaggga ggttgcggag ctgcagcacc
901ggaaccaggc gctgactagc ctggcccagg aggcacaggc cctgaaggat gagatggatg
961aactacggca gtcttcggag cgtgctgggc agctggaggc cacgctgacc agttgccggc
1021gccgcttggg cgagctgagg gagctgcggc ggcaggtgcg gcagctggag gaacgcaacg
1081ccggccacgc cgagcgcacg cgacaactgg aggatgagct acgccgagcg ggctccctgc
1141gcgcccagct ggaggcgcag cggcggcagg tgcaggaact gcagggccag cggcaggagg
1201aggccatgaa ggccgagaaa tggctatttg aatgccgcaa cctggaggaa aagtatgagt
1261cggtgacaaa ggagaaggag cggctgttgg cggagcggga ctccttgcgg gaggccaatg
1321aggagctgcg ctgcgcccag ctgcagccgc gggggttgac ccaggccgat ccctcactgg
1381atcccacctc cacacccgtg gataacttag ccgcagagat cctgcctgcg gagctcaggg
1441agacgctcct gcggcttcag ctggagaaca agcggctgtg caggcaggag gcggccgacc
1501gggagcggca ggaggagctg cagcgccagc tggaggatgc caaccgcgcg cgccacgggt
1561tggagacgca gcaccggctg aaccagcagc agctatccga gctgcgggcc caggtggagg
1621acctgcagaa agccctgcag gagcaggggg gcaagactga agattccatt ttgctgaaaa
1681ggaagctgga ggaacatttg cagaagcTTC ATGAGGCAGA TCTGGAGTTG CAGAGGAAGC
1741GGGAGTACAT TGAGGAGCTG GAGCCACCCA CTGACAGCAG CACAGCCCGG CGGATCGAGG
1801AGCTGCAGCA TAACTTGCAG AAGAAGGACG CGGACTTGCG GGCCATGGAG GAGCGATACC
1861GCCGCTACGT GGACAAGGCC CGCATGGTCA TGCAGACCAT GGAACCCAAG CAGCGGCCAG
1921CTGCGGGGGC ACCTCCAGAA CTCCATTCCC TGAGGACACA GCTCCGAGAA CGGGATGTCC
1981GCATCCGACA CCTGGAGATG GACTTTGAGA AAAGCCGAAG TCAGCGGGAG CAGGAAGAAA
2041AGCTGCTCAT CAGTGCCTGG TATAATATGG GCATGGCCTT GCAGCAGCGA GCTGGGGAGG
2101AGCGGGCGCC TGCCCATGCC CAGTCATTCC TGGCACAGCA GCGGCTGGCA ACCAATTCTC
2161GCCGTGGACC CTTGGGACGC CTGGCATCTC TGAACCTTCG CCCCACTGAC AAGCACTGCC
2221CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2281TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2341ATATATCTTG TGGAAAGGAC GAGTGCCTTT CCGCTCGCGT GGTTTCACGC GTTAAGTCga
2401caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)