Construct: ORF TRCN0000474511
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013632.1_s317c1
- Derived from:
- ccsbBroadEn_06517
- DNA Barcode:
- AGTCCACCCGGTGTGAGGAGTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCP1 (3936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474511
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3936 | LCP1 | lymphocyte cytosolic protein 1 | NM_002298.5 | 99.9% | 99.8% | 1597A>G |
| 2 | human | 3936 | LCP1 | lymphocyte cytosolic protein 1 | XM_005266374.2 | 99.9% | 99.8% | 1597A>G |
| 3 | mouse | 18826 | Lcp1 | lymphocyte cytosolic protein 1 | NM_001247984.1 | 89.4% | 96.9% | (many diffs) |
| 4 | mouse | 18826 | Lcp1 | lymphocyte cytosolic protein 1 | NM_008879.4 | 89.4% | 96.9% | (many diffs) |
| 5 | mouse | 18826 | Lcp1 | lymphocyte cytosolic protein 1 | XM_006518699.1 | 89.4% | 96.9% | (many diffs) |
| 6 | mouse | 18826 | Lcp1 | lymphocyte cytosolic protein 1 | XM_006518701.3 | 89.4% | 96.9% | (many diffs) |
| 7 | mouse | 18826 | Lcp1 | lymphocyte cytosolic protein 1 | XM_006518703.4 | 89.4% | 96.9% | (many diffs) |
| 8 | mouse | 18826 | Lcp1 | lymphocyte cytosolic protein 1 | XM_006518704.1 | 89.4% | 96.9% | (many diffs) |
| 9 | mouse | 18826 | Lcp1 | lymphocyte cytosolic protein 1 | XM_017315920.1 | 89.4% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1947
- ORF length:
- 1881
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagaggatca gtgtccgatg aggaaatgat ggagctcaga gaagcttttg 121 ccaaagttga tactgatggc aatggataca tcagcttcaa tgagttgaat gacttgttca 181 aggctgcttg cttgcctttg cctgggtata gagtacgaga aattacagaa aacctgatgg 241 ctacaggtga tctggaccaa gatggaagga tcagctttga tgagtttatc aagattttcc 301 atggcctaaa aagcacagat gttgccaaga cctttagaaa agcaatcaat aagaaggaag 361 ggatttgtgc aatcggtggt acttcagagc agtctagcgt tggcacccaa cactcctatt 421 cagaggaaga aaagtatgcc tttgtcaact ggataaacaa agccctggaa aatgatcctg 481 attgtcggca tgtcatccca atgaacccaa acacgaatga tctctttaat gctgttggag 541 atggcattgt cctttgtaaa atgatcaacc tgtcagtgcc agacacaatt gatgaaagaa 601 caatcaacaa aaagaagcta acccctttca ccattcagga aaatctgaac ttggctctga 661 actctgcctc agccatcggg tgccatgtgg tcaacatagg ggctgaggac ctgaaggagg 721 ggaagcctta tctggtcctg ggacttctgt ggcaagtcat caagattggg ttgtttgctg 781 acattgaact cagcagaaat gaagctctga ttgctctttt gagagaaggt gagagcctgg 841 aggatttgat gaaactctcc cctgaagagc tcttgctgag gtgggctaat taccacctgg 901 aaaatgcagg ctgcaacaaa attggcaact tcagtactga catcaaggac tcaaaagctt 961 attaccacct gcttgagcag gtggctccaa aaggagatga agaaggtgtt cctgctgttg 1021 ttattgacat gtcaggactg cgggagaagg atgacatcca gagggcagaa tgcatgctgc 1081 agcaggcgga gaggctgggc tgccggcagt ttgtcacagc cacagatgtt gtccgaggga 1141 accccaagtt gaacttggct tttattgcca acctctttaa cagataccct gccctgcaca 1201 aaccagagaa ccaggacatt gactgggggg ctcttgaagg tgagacgaga gaagagcgga 1261 catttaggaa ctggatgaac tccctgggtg ttaaccctcg agtcaatcat ttgtacagtg 1321 acttatcaga tgccctggtc atcttccagc tctatgaaaa gatcaaagtt cctgttgact 1381 ggAACAGAGT AAACAAACCG CCATACCCCA AACTGGGAGG CAATATGAAG AAGCTTGAGA 1441 ATTGTAACTA CGCGGTAGAA TTGGGGAAGA ATCAAGCGAA GTTCTCCCTG GTTGGCATCG 1501 GTGGACAAGA TCTCAATGAA GGAAACCGCA CTCTCACACT GGCCTTGATT TGGCAGCTAA 1561 TGAGAAGGTA TACACTGAAT ATCCTCGAAG AAATTGGTGG TGGCCAGAAG GTCAATGATG 1621 ACATTATTGT CAACTGGGTG AATGAAACAT TGAGGGAAGC AGAGAAAAGT TCATCCATCT 1681 CTAGTTTCAA GGACCCGAAG ATTAGTACAA GTCTGCCTGT TCTGGACCTC ATCGATGCCA 1741 TCCAACCAGG TTCCATTAAC TATGACCTTC TGAAGACAGA AAATCTGAAT GATGATGAGA 1801 AACTCAACAA TGCAAAATAT GCCATCTCTA TGGCCCGAAA AATTGGAGCA AGAGTGTATG 1861 CCCTGCCAGA AGACCTGGTT GAAGTGAACC CCAAAATGGT CATGACCGTG TTTGCCTGCC 1921 TCATGGGGAA AGGAATGAAG AGGGTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1981 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2041 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGTCCACC 2101 CGGTGTGAGG AGTATTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2161 aagatt