Construct: ORF TRCN0000474518

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011811.4_s317c1
Derived from:
ccsbBroadEn_06695
DNA Barcode:
ATCTCACTTGAACCCTGTGTCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCK2 (5106)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474518
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5106 PCK2 phosphoenolpyruvate carboxy... NM_004563.4 100% 100%
2 human 5106 PCK2 phosphoenolpyruvate carboxy... NM_001291556.2 79% 79% 0_1ins402
3 human 5106 PCK2 phosphoenolpyruvate carboxy... NM_001308054.2 79% 79% 0_1ins402
4 human 5106 PCK2 phosphoenolpyruvate carboxy... XM_006720158.3 77.4% 76.5% (many diffs)
5 human 5106 PCK2 phosphoenolpyruvate carboxy... NM_001018073.3 67.6% 64.9% (many diffs)
6 mouse 74551 Pck2 phosphoenolpyruvate carboxy... NM_028994.2 85.1% 88.4% (many diffs)
7 mouse 74551 Pck2 phosphoenolpyruvate carboxy... XM_006519632.2 71.8% 73.1% (many diffs)
8 mouse 74551 Pck2 phosphoenolpyruvate carboxy... XM_006519633.2 49.5% 52.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1986
ORF length:
1920
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cgcattgtac cgccctggcc tgcggcttaa ctggcatggg ctgagcccct
121tgggctggcc atcatgccgt agcatccaga ccctgcgagt gcttagtgga gatctgggcc
181agcttcccac tggcattcga gattttgtag agcacagtgc ccgcctgtgc caaccagagg
241gcatccacat ctgtgatgga actgaggctg agaatactgc cacactgacc ctgctggagc
301agcagggcct catccgaaag ctccccaagt acaataactg ctggctggcc cgcacagacc
361ccaaggatgt ggcacgagta gagagcaaga cggtgattgt aactccttct cagcgggaca
421cggtaccact cccgcctggt ggggcccgtg ggcagctggg caactggatg tccccagctg
481atttccagcg agctgtggat gagaggtttc caggctgcat gcagggccgc accatgtatg
541tgcttccatt cagcatgggt cctgtgggct ccccgctgtc ccgcatcggg gtgcagctca
601ctgactcagc ctatgtggtg gcaagcatgc gtattatgac ccgactgggg acacctgtgc
661ttcaggccct gggagatggt gactttgtca agtgtctgca ctccgtgggc cagcccctga
721caggacaagg ggagccagtg agccagtggc cgtgcaaccc agagaaaacc ctgattggcc
781acgtgcccga ccagcgggag atcatctcct tcggcagcgg ctatggtggc aactccctgc
841tgggcaagaa gtgctttgcc ctacgcatcg cctctcggct ggcccgggat gagggctggc
901tggcagagca catgctgatc ctgggcatca ccagccctgc agggaagaag cgctatgtgg
961cagccgcctt ccctagtgcc tgtggcaaga ccaacctggc tatgatgcgg cctgcactgc
1021caggctggaa agtggagtgt gtgggggatg atattgcttg gatgaggttt gacagtgaag
1081gtcgactccg ggccatcaac cctgagaacg gcttctttgg ggttgcccct ggtacctctg
1141ccaccaccaa tcccaacgcc atggctacaa tccagagtaa cactattttt accaatgtgg
1201ctgagaccag tgatggtggc gtgtactggg agggcattga ccagcctctt ccacctggtg
1261ttactgtgac ctcctggctg ggcaaaccct ggaaacctgg tgacaaggag ccctgtgcac
1321atcccaactc tcgattttgt gccccggctc gccagtgccc catcatggac ccagcctggg
1381aggccccaga gggtgtcccc attgacgcca tcatctttgg tggccgcaga cccaaagggg
1441tacccctggt atacgaggcc ttcaactggc gtcatggggt gtttgtgggc agcgccatgc
1501gctctgagtc cactgctgca gcagaacaca aagggaagat catcatgCAC GACCCATTTG
1561CCATGCGGCC CTTTTTTGGC TACAACTTCG GGCACTACCT GGAACACTGG CTGAGCATGG
1621AAGGGCGCAA GGGGGCCCAG CTGCCCCGTA TCTTCCATGT CAACTGGTTC CGGCGTGACG
1681AGGCAGGGCA CTTCCTGTGG CCAGGCTTTG GGGAGAATGC TCGGGTGCTA GACTGGATCT
1741GCCGGCGGTT AGAGGGGGAG GACAGTGCCC GAGAGACACC CATTGGGCTG GTGCCAAAGG
1801AAGGAGCCTT GGATCTCAGC GGCCTCAGAG CTATAGACAC CACTCAGCTG TTCTCCCTCC
1861CCAAGGACTT CTGGGAACAG GAGGTTCGTG ACATTCGGAG CTACCTGACA GAGCAGGTCA
1921ACCAGGATCT GCCCAAAGAG GTGTTGGCTG AGCTTGAGGC CCTGGAGAGA CGTGTGCACA
1981AAATGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2041TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2101CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCTCACTT GAACCCTGTG TCAATACGCG
2161TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)