Construct: ORF TRCN0000474530
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014753.1_s317c1
- Derived from:
- ccsbBroadEn_01099
- DNA Barcode:
- CTGTTACGGCCGAGCGTTCAACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NINJ1 (4814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474530
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4814 | NINJ1 | ninjurin 1 | NM_004148.4 | 100% | 100% | |
| 2 | human | 4814 | NINJ1 | ninjurin 1 | XM_011518716.1 | 67.1% | 67.1% | 0_1ins150 |
| 3 | mouse | 18081 | Ninj1 | ninjurin 1 | NM_013610.2 | 87% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctcgggaacc gaggagtacg agctcaacgg cggcctgcct ccgggcacac 121 ccggctcccc ggacgcctcg ccggcccgct ggggctggag gcacgggccc atcaacgtga 181 accattacgc cagcaagaag agcgcagccG AGAGCATGCT GGACATCGCG CTGCTGATGG 241 CCAACGCGTC CCAGCTGAAG GCCGTCGTGG AACAGGGCCC CAGCTTCGCC TTCTATGTGC 301 CCCTGGTGGT CCTCATCTCC ATCTCCCTTG TGCTGCAGAT CGGCGTGGGG GTGCTGCTCA 361 TCTTCCTTGT CAAGTACGAC CTTAACAACC CGGCCAAGCA CGCCAAGCTG GACTTCCTCA 421 ACAACCTGGC CACGGGCCTG GTGTTCATCA TCGTGGTAGT CAACATCTTC ATCACGGCCT 481 TCGGGGTCCA GAAGCCCTTG ATGGACATGG CACCCCAGCA GTACCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGACTG TTACGGCCGA GCGTTCAACT CACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t