Construct: ORF TRCN0000474533
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014408.1_s317c1
- Derived from:
- ccsbBroadEn_06847
- DNA Barcode:
- GGCCTAGCATACCTGGTGTTGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBBP4 (5928)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474533
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5928 | RBBP4 | RB binding protein 4, chrom... | NM_005610.3 | 99.8% | 100% | 453T>C;795A>C |
| 2 | human | 5928 | RBBP4 | RB binding protein 4, chrom... | NM_001135255.2 | 99.6% | 99.7% | 14_15insAGC;450T>C;792A>C |
| 3 | human | 5928 | RBBP4 | RB binding protein 4, chrom... | NM_001135256.1 | 91.6% | 91.7% | 0_1ins105;348T>C;690A>C |
| 4 | mouse | 19646 | Rbbp4 | retinoblastoma binding prot... | NM_009030.3 | 92% | 100% | (many diffs) |
| 5 | mouse | 19646 | Rbbp4 | retinoblastoma binding prot... | XR_001784111.1 | 63.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgacaaggaa gcagccttcg acgacgcagt ggaagaacga gtgatcaacg 121 aggaatacaa aatatggaaa aagaacaccc cttttcttta tgatttggtg atgacccatg 181 ctctggagtg gcccagccta actgcccagt ggcttccaga tgtaaccaga ccagaaggga 241 aagatttcag cattcatcga cttgtcctgg ggacacacac atcggatgaa caaaaccatc 301 ttgttatagc cagtgtgcag ctccctaatg atgatgctca gtttgatgcg tcacactacg 361 acagtgagaa aggagaattt ggaggttttg gttcagttag tggaaaaatt gaaatagaaa 421 tcaagatcaa ccatgaagga gaagtaaaca gggcccgtta tatgccccag aacccttgta 481 tcatcgcaac aaagactcct tccagtgatg ttcttgtctt tgactataca aaacatcctt 541 ctaaaccaga tccttctgga gagtgcaacc cagacttgcg tctccgtgga catcagaagg 601 aaggctatgg gctttcttgg aacccaaatc tcagtgggca cttacttagt gcttcagatg 661 accataccat ctgcctgtgg gacatcagtg ccgttccaaa ggagggaaaa gtggtagatg 721 cgaagaccat ctttacaggg catacggcag tagtagaaga tgtttcctgg catctactcc 781 atgagtctct gtttgggTCA GTTGCTGATG ATCAGAAACT TATGATTTGG GATACTCGTT 841 CAAACAATAC TTCCAAACCC AGCCACTCAG TTGATGCTCA CACTGCTGAA GTGAACTGCC 901 TTTCTTTCAA TCCTTATAGT GAGTTCATTC TTGCCACAGG ATCAGCTGAC AAGACTGTTG 961 CCTTGTGGGA TCTGAGAAAT CTGAAACTTA AGTTGCATTC CTTTGAGTCA CATAAGGATG 1021 AAATATTCCA GGTTCAGTGG TCACCTCACA ATGAGACTAT TTTAGCTTCC AGTGGTACTG 1081 ATCGCAGACT GAATGTCTGG GATTTAAGTA AAATTGGAGA GGAACAATCC CCAGAAGATG 1141 CAGAAGACGG GCCACCAGAG TTGTTGTTTA TTCATGGTGG TCATACTGCC AAGATATCTG 1201 ATTTCTCCTG GAATCCCAAT GAACCTTGGG TGATTTGTTC TGTATCAGAA GACAATATCA 1261 TGCAAGTGTG GCAAATGGCA GAGAACATTT ATAATGATGA AGACCCTGAA GGAAGCGTGG 1321 ATCCAGAAGG ACAAGGGTCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGCC TAGCATACCT 1501 GGTGTTGGAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt