Transcript: Human NM_001135255.2

Homo sapiens RB binding protein 4, chromatin remodeling factor (RBBP4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
RBBP4 (5928)
Length:
7880
CDS:
100..1374

Additional Resources:

NCBI RefSeq record:
NM_001135255.2
NBCI Gene record:
RBBP4 (5928)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001135255.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380311 ATTTGGGATACTCGTTCAAAC pLKO_005 856 CDS 100% 10.800 15.120 N RBBP4 n/a
2 TRCN0000381646 CCTTGTATCATCGCAACAAAG pLKO_005 505 CDS 100% 10.800 15.120 N RBBP4 n/a
3 TRCN0000293556 TGGTCATACTGCCAAGATATC pLKO_005 1209 CDS 100% 10.800 15.120 N RBBP4 n/a
4 TRCN0000115869 CCCTTGTATCATCGCAACAAA pLKO.1 504 CDS 100% 5.625 7.875 N RBBP4 n/a
5 TRCN0000293554 ATGCGTCACACTACGACAGTG pLKO_005 377 CDS 100% 4.050 5.670 N RBBP4 n/a
6 TRCN0000115871 GCAGACTGAATGTCTGGGATT pLKO.1 1115 CDS 100% 0.405 0.324 N RBBP4 n/a
7 TRCN0000115867 CCTCTTGGTTTCTTCAGTTAA pLKO.1 1881 3UTR 100% 13.200 9.240 N RBBP4 n/a
8 TRCN0000286104 CCTCTTGGTTTCTTCAGTTAA pLKO_005 1881 3UTR 100% 13.200 9.240 N RBBP4 n/a
9 TRCN0000380596 GCATTCCTTTGAGTCACATAA pLKO_005 1026 CDS 100% 13.200 9.240 N RBBP4 n/a
10 TRCN0000115870 GCCTTTCTTTCAATCCTTATA pLKO.1 929 CDS 100% 13.200 9.240 N RBBP4 n/a
11 TRCN0000286103 GCCTTTCTTTCAATCCTTATA pLKO_005 929 CDS 100% 13.200 9.240 N RBBP4 n/a
12 TRCN0000379863 ACGAGTGATCAACGAGGAATA pLKO_005 138 CDS 100% 10.800 7.560 N RBBP4 n/a
13 TRCN0000293555 ATGAACCTTGGGTGATTTGTT pLKO_005 1250 CDS 100% 5.625 3.938 N RBBP4 n/a
14 TRCN0000115868 CGGCAGTAGTAGAAGATGTTT pLKO.1 776 CDS 100% 5.625 3.375 N RBBP4 n/a
15 TRCN0000072628 CCTCCCAAAGTGCTAGGATTA pLKO.1 5556 3UTR 100% 10.800 5.400 Y MRPS16 n/a
16 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 4179 3UTR 100% 4.950 2.475 Y ERAP2 n/a
17 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4180 3UTR 100% 13.200 6.600 Y LIAS n/a
18 TRCN0000140657 CCTCCCAAAGTGCTAGGATAA pLKO.1 5556 3UTR 100% 10.800 5.400 Y CD3EAP n/a
19 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6776 3UTR 100% 5.625 2.813 Y KLHL30 n/a
20 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6776 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001135255.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06847 pDONR223 100% 99.6% 99.7% None 14_15insAGC;450T>C;792A>C n/a
2 ccsbBroad304_06847 pLX_304 0% 99.6% 99.7% V5 14_15insAGC;450T>C;792A>C n/a
3 TRCN0000474533 GGCCTAGCATACCTGGTGTTGGAG pLX_317 42.7% 99.6% 99.7% V5 14_15insAGC;450T>C;792A>C n/a
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