Construct: ORF TRCN0000474540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000517.1_s317c1
- Derived from:
- ccsbBroadEn_08190
- DNA Barcode:
- TGGTGAACCTCGTGCATGCCCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF141 (50862)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50862 | RNF141 | ring finger protein 141 | NM_016422.4 | 99.8% | 100% | 117T>C |
| 2 | mouse | 67150 | Rnf141 | ring finger protein 141 | NM_025999.3 | 92.4% | 99.1% | (many diffs) |
| 3 | mouse | 67150 | Rnf141 | ring finger protein 141 | XM_006508123.2 | 92.4% | 99.1% | (many diffs) |
| 4 | mouse | 67150 | Rnf141 | ring finger protein 141 | XM_006508124.2 | 92.4% | 99.1% | (many diffs) |
| 5 | mouse | 67150 | Rnf141 | ring finger protein 141 | XM_006508125.2 | 71.4% | 77.3% | (many diffs) |
| 6 | mouse | 67150 | Rnf141 | ring finger protein 141 | XR_869742.1 | 23.3% | (many diffs) | |
| 7 | mouse | 67150 | Rnf141 | ring finger protein 141 | XR_001777995.1 | 9.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggaatggg acagcaaatt tcggatcaga cacagttggt tattaacaag ttaccagaaa 121 aagtagcaaa acatgttacg ttggttcgag agagtggctc cttaacttat gaagaatttc 181 tcgggagagt agctgagctt aatgatgtaa cggctaaagt ggcttctggc caggaaaaac 241 atcttctctt tgaggtacaa cctgGGTCTG ATTCCTCTGC TTTTTGGAAA GTGGTTGTAC 301 GGGTGGTCTG TACCAAGATT AACAAAAGCA GTGGCATTGT GGAGGCATCA CGGATCATGA 361 ATTTATACCA GTTTATTCAA CTTTATAAAG ATATCACAAG TCAAGCAGCA GGAGTATTGG 421 CACAGAGCTC CACCTCTGAA GAACCTGATG AAAACTCATC CTCTGTAACA TCTTGTCAGG 481 CTAGTCTTTG GATGGGAAGG GTGAAGCAGC TGACCGATGA GGAGGAGTGT TGTATCTGTA 541 TGGATGGGCG GGCTGACCTC ATCCTGCCTT GTGCTCACAG CTTTTGTCAG AAGTGTATTG 601 ATAAATGGAG TGATCGACAC AGGAATTGCC CTATTTGTCG CCTACAGATG ACTGGAGCAA 661 ATGAATCTTG GGTGGTATCA GATGCACCCA CTGAAGATGA TATGGCTAAC TATATTCTTA 721 ACATGGCTGA TGAGGCAGGC CAGCCCCACA GGCCATGCCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 ATGGTGAACC TCGTGCATGC CCGTCACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt