Construct: ORF TRCN0000474541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015412.1_s317c1
- Derived from:
- ccsbBroadEn_04382
- DNA Barcode:
- TTTATCAGGATCAGTTTGGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYDC2 (84332)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84332 | DYDC2 | DPY30 domain containing 2 | NM_001270041.1 | 100% | 100% | |
| 2 | human | 84332 | DYDC2 | DPY30 domain containing 2 | NM_032372.6 | 100% | 100% | |
| 3 | human | 84332 | DYDC2 | DPY30 domain containing 2 | XM_011540267.2 | 100% | 100% | |
| 4 | human | 84332 | DYDC2 | DPY30 domain containing 2 | XM_011540268.1 | 100% | 100% | |
| 5 | human | 84332 | DYDC2 | DPY30 domain containing 2 | NM_001270042.1 | 92.6% | 92.6% | 1_42del |
| 6 | human | 84332 | DYDC2 | DPY30 domain containing 2 | XM_011540270.2 | 71.3% | 70% | (many diffs) |
| 7 | human | 84332 | DYDC2 | DPY30 domain containing 2 | NR_070309.1 | 25.8% | 1_380del;912_2052del | |
| 8 | human | 84332 | DYDC2 | DPY30 domain containing 2 | NR_070308.1 | 23.3% | 1_598del;1130_2270del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 597
- ORF length:
- 531
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aactaactac ctgaagaggt gctttggaaa ttgcctggcc caggcactgg 121 cagaggtggc gaaggttcgg cccagtgacc caatagaata cctggctcac tggctttatc 181 attacaggaa aacagcaaaa gcaaaagaag agaataggga aaagaagatc caccTGCAGG 241 AGGAATATGA CAGTAGCCTC AAGGAAATGG AAATGACAGA AATGCTGAAA CAGGAAGAGT 301 ATCAGATTCA ACAGAACTGT GAAAAGTGTC ACAAGGAACT GACTTCTGAA ACTGTTTCCA 361 CGAAGAAGAC CATATTCATG CAGGAGGACA CAAACCCCCT TGAGAAGGAG GCCTTGAAGC 421 AGGAATTCCT GCCAGGTACT TCCAGTCTGA TTCCAGGAAT GCCTCAACAG GTTCCTCCTT 481 CAGAGTCTGC TGGCCAGATT GACCAGAACT TCAAAATGCC ACAAGAAATA AATTACAAGG 541 AGGCTTTTCA GCATGAAGTT GCTCATGAAA TGCCTCCTGG CTCCAAATCT CCTTTTTACC 601 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 661 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 721 ATATATCTTG TGGAAAGGAC GATTTATCAG GATCAGTTTG GACCCCACGC GTTAAGTCga 781 caatcaacct ctggattaca aaatttgtga aagatt