Construct: ORF TRCN0000474554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008733.1_s317c1
- Derived from:
- ccsbBroadEn_07029
- DNA Barcode:
- CCTCTATGCGGCAGATTTGGTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TADA2A (6871)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6871 | TADA2A | transcriptional adaptor 2A | NM_001166105.3 | 99.9% | 99.7% | 7C>T |
| 2 | human | 6871 | TADA2A | transcriptional adaptor 2A | NM_001488.4 | 99.9% | 99.7% | 7C>T |
| 3 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_017024981.1 | 95.4% | 95.2% | 7C>T;131_132ins60 |
| 4 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_017024982.1 | 87.7% | 87.5% | 7C>T;442_443ins162 |
| 5 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_011525153.2 | 85.7% | 85.7% | 0_1ins189 |
| 6 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_011525154.2 | 85.7% | 85.7% | 0_1ins189 |
| 7 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_006722044.3 | 77.2% | 77.2% | 0_1ins303 |
| 8 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_011525155.2 | 77.2% | 77.2% | 0_1ins303 |
| 9 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_017024983.1 | 77.2% | 77.2% | 0_1ins303 |
| 10 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_017024984.1 | 77.2% | 77.2% | 0_1ins303 |
| 11 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_017024985.1 | 77.2% | 77.2% | 0_1ins303 |
| 12 | human | 6871 | TADA2A | transcriptional adaptor 2A | NM_001291918.2 | 66% | 60.6% | (many diffs) |
| 13 | human | 6871 | TADA2A | transcriptional adaptor 2A | NM_133439.4 | 66% | 60.6% | (many diffs) |
| 14 | human | 6871 | TADA2A | transcriptional adaptor 2A | XM_017024986.1 | 53.9% | 53.9% | 0_1ins612 |
| 15 | mouse | 217031 | Tada2a | transcriptional adaptor 2A | NM_172562.3 | 90.6% | 97% | (many diffs) |
| 16 | mouse | 217031 | Tada2a | transcriptional adaptor 2A | XM_006532989.3 | 86.7% | 92.5% | (many diffs) |
| 17 | mouse | 217031 | Tada2a | transcriptional adaptor 2A | XM_006532991.1 | 57.1% | 61.3% | (many diffs) |
| 18 | mouse | 217031 | Tada2a | transcriptional adaptor 2A | XM_011248932.2 | 56.1% | 60% | (many diffs) |
| 19 | mouse | 217031 | Tada2a | transcriptional adaptor 2A | XR_001779958.1 | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ctgtttgggt tcctttagca atgatccctc tgataagcca ccttgccgag 121 gctgctcctc ctacctcatg gagccttata tcaagtgtgc tgaatgtggg ccacctcctt 181 ttttcctctg cttgcagtgt ttcactcgag gctttgagta caagaaacat caaagcgatc 241 atacttatga aataatgact tcagattttc ctgtccttga tcccagctgg actgctcaag 301 aagaaatggc ccttttagaa gctgtgatgg actgtggctt tggaaattgg caggatgtag 361 ccaatcaaat gtgcaccaag accaaggagg agtgtgagaa gcactatatg aagcatttca 421 tcaataaccc tctgtttgca tctaccctgc tgaacctgaa acaagcagag gaagcaaaaa 481 ctgctgacac agccattcca tttcactcta cagatgaccc tccccgacct acctttgact 541 ccttgctttc tcgggacatg gccgggtaca tgccagctcg agcagatttc attgaggaat 601 ttgacaatta tgcagaatgg gacttgagag acattgattt tgttgaagat gactcggaca 661 ttttacatgc tctgaagatg gctgtggtag atatctatca ttccaggtta aaggagagac 721 aaagacgaaa aaaaattata agagaccatg gattaatcaa ccttagaaag tttcaattaa 781 tggaacggcg gtatcccaag gaggtccagg acctgtatGA AACAATGAGG CGATTTGCAA 841 GAATTGTGGG GCCAGTGGAA CATGACAAAT TCATTGAAAG CCATGCATTG GAATTTGAAC 901 TCCGAAGGGA AATCAAGAGG CTCCAAGAAT ACAGGACAGC AGGCATTACC AATTTTTGTA 961 GTGCCAGAAC CTACGATCAC CTCAAGAAGA CACGGGAGGA AGAGCGCCTT AAACGCACTA 1021 TGCTCTCAGA AGTTCTCCAG TATATCCAGG ACAGTAGTGC TTGCCAGCAG TGGCTCCGCC 1081 GGCAAGCTGA CATTGATTCC GGCCTGAGTC CTTCCATTCC AATGGCTTCG AATTCAGGTA 1141 GACGGAGTGC ACCACCCTTG AACCTCACTG GCCTCCCTGG CACAGAGAAG CTGAATGAAA 1201 AAGAAAAGGA GCTCTGTCAG ATGGTGAGGT TGGTCCCTGG AGCCTATTTA GAATACAAAT 1261 CTGCTCTATT GAACGAATGT AACAAGCAAG GAGGCTTAAG ACTGGCGCAG GCAAGAGCAC 1321 TCATCAAGAT AGATGTGAAC AAAACCCGGA AAATCTATGA TTTCCTCATC AGAGAAGGAT 1381 ACATCACTAA AGGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1441 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1501 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCTCTATGCG GCAGATTTGG 1561 TTGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt