Construct: ORF TRCN0000474564
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012494.1_s317c1
- Derived from:
- ccsbBroadEn_05743
- DNA Barcode:
- GCGGCATACTTCGGGCCGAACAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEKT4P2 (100132288)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474564
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100132288 | TEKT4P2 | tektin 4 pseudogene 2 | NR_038328.2 | 53.5% | (many diffs) | |
| 2 | human | 100233156 | LOC100233156 | tektin 4 pseudogene | NR_037871.1 | 49.4% | (many diffs) | |
| 3 | human | 150483 | TEKT4 | tektin 4 | NM_001286559.2 | 44.2% | 37.8% | (many diffs) |
| 4 | human | 727764 | MAFIP | MAFF interacting protein (p... | NR_046440.2 | 40.8% | 1_90del;322C>T;478_946del | |
| 5 | human | 150483 | TEKT4 | tektin 4 | NM_144705.4 | 25.8% | 22.4% | (many diffs) |
| 6 | human | 100132288 | TEKT4P2 | tektin 4 pseudogene 2 | NR_038327.2 | 23.8% | 1_81del;313C>T;469_1617del | |
| 7 | human | 150483 | TEKT4 | tektin 4 | XM_011510670.2 | 23.7% | 20.5% | (many diffs) |
| 8 | human | 100233156 | LOC100233156 | tektin 4 pseudogene | NR_037872.1 | 23.5% | (many diffs) | |
| 9 | human | 105379273 | LOC105379273 | uncharacterized LOC105379273 | XR_002958840.1 | 20.7% | (many diffs) | |
| 10 | human | 727764 | MAFIP | MAFF interacting protein (p... | NR_046441.2 | 16.4% | (many diffs) | |
| 11 | human | 727764 | MAFIP | MAFF interacting protein (p... | NR_046439.2 | 13.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 478
- ORF length:
- 387
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAAGTTGGC ATGTTTCCAG TGTCCTCTGG GTGTTTCCAA 121 GAGCAACAAG AAACGAATAA ATCTCTGCCC CGCAGCGCCT CCACCCCAGA GACCCGGACC 181 AAGTTCACAC AGGACAATCT GTGCCACGCC CAGCGCGAGC GCCTGGACTC GGCCAACCTG 241 TGGGTGCTGG TGGACTGCAT CCTTCGCGAC ACCTCCGAGG ACCTGGGACT CCAGTGTGAC 301 GCCGTGAACC TGGCCTTCGG GTGCCGCTGT GAGGAACTGG AGGACGCGCG GCACAAGCTG 361 CAGCACCACC TGCACAAGAT GCTGCGGGAA ATCACAGATC AGGAACACAA CGTGGTGGCA 421 CTGAAGGAGG CCATCAAGGA CAAGGAGGAA CCTCTGCACA TAGCCCAGAC CCGGCTGTAC 481 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 541 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 601 TATATATCTT GTGGAAAGGA CGAGCGGCAT ACTTCGGGCC GAACAGTACG CGTTAAGTCg 661 acaatcaacc tctggattac aaaatttgtg aaagatt