Construct: ORF TRCN0000474579
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005976.1_s317c1
- Derived from:
- ccsbBroadEn_06648
- DNA Barcode:
- TTCGTTGTACTGGATTTCGGACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNOT3 (4849)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474579
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | NM_014516.4 | 99.9% | 100% | 1122T>G |
| 2 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XM_005278282.3 | 99.8% | 99.8% | 484_486delCAG;1125T>G |
| 3 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XM_005278281.2 | 97.7% | 97.7% | 1122T>G;2161_2211del |
| 4 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XM_005278279.2 | 97.6% | 97.6% | 484_486delCAG;1125T>G;2164_2214del |
| 5 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XM_005278280.2 | 97.6% | 97.6% | 484_486delCAG;1125T>G;2164_2214del |
| 6 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XM_011526992.2 | 96.6% | 92.4% | (many diffs) |
| 7 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XM_011526993.3 | 90.2% | 90% | (many diffs) |
| 8 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XR_254515.3 | 77.2% | (many diffs) | |
| 9 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XR_002958318.1 | 77.1% | (many diffs) | |
| 10 | human | 4849 | CNOT3 | CCR4-NOT transcription comp... | XR_002958319.1 | 76.4% | (many diffs) | |
| 11 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | NM_146176.3 | 90.9% | 95.2% | (many diffs) |
| 12 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_011250501.2 | 90.9% | 95.2% | (many diffs) |
| 13 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_017322146.1 | 90.9% | 95.2% | (many diffs) |
| 14 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_006539752.3 | 90.8% | 95% | (many diffs) |
| 15 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_006539753.3 | 90.8% | 95% | (many diffs) |
| 16 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_006539755.3 | 90.8% | 95% | (many diffs) |
| 17 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_006539756.3 | 90.8% | 95% | (many diffs) |
| 18 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_011250500.2 | 90.8% | 95% | (many diffs) |
| 19 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_017322145.1 | 90.8% | 95% | (many diffs) |
| 20 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XM_006539757.3 | 83.6% | 84.7% | (many diffs) |
| 21 | mouse | 232791 | Cnot3 | CCR4-NOT transcription comp... | XR_001785521.1 | 59.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2325
- ORF length:
- 2259
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacaagcgc aaactccaag gtgagattga tcgctgcctc aagaaggtgt 121 ccgagggcgt ggagcagttt gaagatattt ggcagaagct ccacaatgca gccaacgcga 181 accagaaaga aaagtatgag gctgacctaa agaaggagat taagaagcta caacggctga 241 gggaccaaat caagacatgg gtagcgtcca acgagatcaa ggacaagagg cagcttatag 301 acaaccgcaa gctcattgag acgcaaatgg aacggttcaa agttgtggaa cgagagacca 361 aaaccaaagc ttacagcaaa gagggcctgg gcctggccca gaaggtagat cctgcccaga 421 aggagaagga agaggttggc cagtggctca cgaataccat cgacacgctc aacatgcagg 481 tggaccagtt tgagagtgaa gtggagtcac tgtcagtgca gacacgcaag aagaagggcg 541 acaaggataa gcaggaccgg attgagggct tgaagcggca catcgagaag caccgctacc 601 acgtgcgcat gctagagacc atcctgcgca tgctggacaa tgactccatc ctcgttgacg 661 ccatccgcaa gatcaaggac gacgttgagt actatgttga ctcatcccag gaccccgact 721 tcgaggagaa cgagtttctc tacgatgacc tggacctcga ggacattcca caggcgctgg 781 tcgccacctc cccccccagc cacagccaca tggaggatga gatcttcaac cagtccagca 841 gcacgcccac ctcaaccacc tccagctctc ccatcccgcc cagcccagcc aactgtacca 901 cggaaaactc tgaagatgat aagaagaggg gacgttccac agacagtgaa gtcagccagt 961 ctccagccaa aaacggctcc aagcctgtcc acagcaacca gcaccctcag tccccagctg 1021 tgccgcccac ctacccctcc ggccccccgc ctgctgcctc tgccttgagc accactcctg 1081 gcaacaatgg ggtccccgcc cccgcagcac ccccaagtgc cctgggcccc aaggccagtc 1141 cagctcccag ccacaactcg ggcacccctg ctccctatgc ccaggcggtg gccccaccag 1201 ctcccagtgg gcccagcacg acccagcccc ggccccccag cgtccagcct agcggaggcg 1261 gaggcggcgg cagcggaggc ggagggagca gcagcagtag taacagcagt gccggtggag 1321 gggctggcaa gcagaatggc gccaccagtt acagctcagt tgtggcagac agcccggcag 1381 aggtggcttt gagcagcagt gggggcaaca atgccagcag ccaggccttg ggcccccctt 1441 ccggccccca caacccacct cccagcacct cgaaggaacc cagtgcggca gccccaacgg 1501 gggctggggg cgtggcccca ggctcaggga acaactcagg gggacccagc ctcctggtgc 1561 cactgcctgt gaatcctccc agctccccaa cgcccagctt cagtgatgcc aaggcagccg 1621 gtgccctgct caatgggcct ccacagttca gcaccgcccc agaaatcaag gcccctgagc 1681 ctctgagctc cttgaagtcc atggcggaac gggcagccat cagctctggc attgaggacc 1741 ctgtgccaac gctgcacctg accgagcgag acatcatcct gagcagtaca tcagcacctc 1801 cggcctcagc ccagccgccc ctGCAGCTGT CAGAGGTGAA CATACCGCTG TCGCTGGGTG 1861 TCTGTCCACT GGGCCCTGTG CCCCTCACCA AGGAGCAGCT CTATCAGCAG GCCATGGAAG 1921 AGGCCGCCTG GCACCACATG CCTCACCCCT CTGACTCTGA GCGTATTCGG CAGTACCTCC 1981 CCCGGAACCC CTGTCCGACG CCCCCCTACC ACCACCAGAT GCCACCCCCA CACTCGGACA 2041 CTGTGGAATT CTACCAGCGC CTGTCGACCG AGACACTCTT CTTCATCTTC TACTATCTGG 2101 AGGGCACTAA GGCACAGTAT CTGGCAGCCA AGGCCCTAAA GAAGCAGTCA TGGCGATTCC 2161 ACACCAAGTA CATGATGTGG TTCCAGAGGC ACGAGGAGCC CAAGACCATC ACTGACGAGT 2221 TTGAGCAGGG CACCTACATC TACTTTGACT ACGAGAAGTG GGGCCAGCGG AAGAAGGAAG 2281 GCTTCACCTT TGAGTACCGC TACCTGGAGG ACCGGGACCT CCAGTGCCCA ACTTTCTTGT 2341 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2401 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2461 GAAAGGACGA TTCGTTGTAC TGGATTTCGG ACTCACGCGT TAAGTCgaca atcaacctct 2521 ggattacaaa atttgtgaaa gatt