Construct: ORF TRCN0000474584
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017963.1_s317c1
- Derived from:
- ccsbBroadEn_02295
- DNA Barcode:
- CCTTCTCAATAACCGTTGCATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGK (10026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474584
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10026 | PIGK | phosphatidylinositol glycan... | NM_005482.3 | 99.9% | 100% | 372C>T |
| 2 | mouse | 329777 | Pigk | phosphatidylinositol glycan... | NM_025662.5 | 83.4% | 91.3% | (many diffs) |
| 3 | mouse | 329777 | Pigk | phosphatidylinositol glycan... | NM_178016.3 | 74.6% | 81.4% | (many diffs) |
| 4 | mouse | 329777 | Pigk | phosphatidylinositol glycan... | XM_006501612.3 | 66.4% | 74.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1251
- ORF length:
- 1185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgtcaccgac agcctcagcc gggctgcgac tgtcttggca actgtgttgc 121 tcttgtcctt cggcagcgtg gccgctagtc atatcgagga tcaagcagaa caattcttta 181 gaagtggcca tacaaacaac tgggctgttc tggtgtgtac atcccgattc tggtttaatt 241 atcgacatgt tgcaaatacc ctttctgttt atagaagtgt caagaggcta ggtattcctg 301 acagtcacat tgtcctaatg cttgcagatg atatggcctg taatcctaga aatcccaaac 361 cagctacagt gtttagtcac aagaatatgg aactaaatgt gtatggagat gatgtggaag 421 tggattatag aagttatgag gtaactgtgg agaatttttt acgggtatta actgggagga 481 tcccacctag tactcctcgg tcaaaacgtc ttctttctga tgacagaagc aatattctaa 541 tttatatgac agggcatggt ggaaatggtt tcttaaaatt tcaagattct gaagaaatta 601 ccaacataga actcgcggat gcttttgaac aaatgtggca gaaaagacgc tacaatgagc 661 tactgtttat tattgatact tgccaaggag catccatGTA TGAACGATTT TATTCTCCTA 721 ACATAATGGC TCTAGCTAGT AGTCAAGTGG GAGAAGATTC ACTCTCGCAT CAACCTGATC 781 CTGCAATTGG AGTCCATCTT ATGGATAGAT ACACATTTTA TGTCTTGGAA TTTTTGGAAG 841 AAATTAACCC AGCTAGCCAA ACTAATATGA ATGACCTTTT TCAGGTATGT CCCAAAAGTC 901 TGTGTGTGTC TACTCCTGGA CATCGCACTG ATCTTTTTCA GAGGGATCCT AAAAATGTAC 961 TGATAACTGA TTTCTTTGGA AGTGTACGGA AAGTGGAAAT TACAACAGAG ACTATTAAAT 1021 TGCAACAGGA TTCAGAAATC ATGGAAAGCA GCTATAAGGA AGACCAGATG GATGAGAAAC 1081 TAATGGAACC TCTGAAATAT GCTGAACAAC TTCCTGTAGC TCAGATAATA CACCAGAAAC 1141 CGAAGCTGAA AGACTGGCAT CCTCCTGGGG GCTTTATTCT GGGATTATGG GCACTTATTA 1201 TCATGGTTTT CTTCAAAACT TATGGAATTA AGCATATGAA GTTCATTTTT TACCCAACTT 1261 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1321 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1381 CTTGTGGAAA GGACGACCTT CTCAATAACC GTTGCATTTT ACGCGTTAAG TCgacaatca 1441 acctctggat tacaaaattt gtgaaagatt