Construct: ORF TRCN0000474598
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018199.1_s317c1
- Derived from:
- ccsbBroadEn_01903
- DNA Barcode:
- ACTACAGCTCACCGGCGTAGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H2BC21 (8349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474598
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8349 | H2BC21 | H2B clustered histone 21 | NM_003528.2 | 100% | 100% | |
| 2 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001024599.4 | 94.4% | 97.6% | (many diffs) |
| 3 | human | 8348 | H2BC17 | H2B clustered histone 17 | NM_003527.4 | 92.8% | 99.2% | (many diffs) |
| 4 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_001312653.1 | 91.5% | 98.4% | (many diffs) |
| 5 | human | 85236 | H2BC12 | H2B clustered histone 12 | NM_080593.2 | 91.5% | 98.4% | (many diffs) |
| 6 | human | 8341 | H2BC15 | H2B clustered histone 15 | NM_003520.3 | 91.2% | 98.4% | (many diffs) |
| 7 | human | 8344 | H2BC6 | H2B clustered histone 6 | NM_003523.2 | 91% | 99.2% | (many diffs) |
| 8 | human | 128312 | H2BU1 | H2B.U histone 1 | NM_175055.2 | 91% | 96% | (many diffs) |
| 9 | human | 8343 | H2BC7 | H2B clustered histone 7 | NM_003522.3 | 90.7% | 99.2% | (many diffs) |
| 10 | human | 8340 | H2BC13 | H2B clustered histone 13 | NM_003519.3 | 90.7% | 97.6% | (many diffs) |
| 11 | human | 54145 | H2BS1 | H2B.S histone 1 | NM_017445.3 | 90.4% | 96.8% | (many diffs) |
| 12 | human | 102724334 | LOC102724334 | histone H2B type F-S-like | XM_006723918.4 | 90.4% | 96.8% | (many diffs) |
| 13 | human | 8347 | H2BC4 | H2B clustered histone 4 | NM_003526.2 | 90.2% | 99.2% | (many diffs) |
| 14 | human | 8346 | H2BC10 | H2B clustered histone 10 | NM_003525.2 | 89.9% | 99.2% | (many diffs) |
| 15 | human | 8970 | H2BC11 | H2B clustered histone 11 | NM_021058.3 | 89.4% | 99.2% | (many diffs) |
| 16 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_021063.3 | 89.4% | 98.4% | (many diffs) |
| 17 | human | 3017 | H2BC5 | H2B clustered histone 5 | NM_138720.2 | 89.4% | 98.4% | (many diffs) |
| 18 | human | 440689 | H2BC18 | H2B clustered histone 18 | NM_001161334.1 | 88.8% | 91.7% | (many diffs) |
| 19 | human | 8345 | H2BC9 | H2B clustered histone 9 | NM_003524.2 | 88.6% | 98.4% | (many diffs) |
| 20 | human | 8342 | H2BC14 | H2B clustered histone 14 | NM_003521.2 | 88.3% | 96% | (many diffs) |
| 21 | human | 3018 | H2BC3 | H2B clustered histone 3 | NM_021062.2 | 87.5% | 98.4% | (many diffs) |
| 22 | human | 8339 | H2BC8 | H2B clustered histone 8 | NM_003518.3 | 86.7% | 99.2% | (many diffs) |
| 23 | human | 3017 | H2BC5 | H2B clustered histone 5 | XM_005249039.4 | 75.1% | 82.6% | (many diffs) |
| 24 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160944.2 | 70.7% | (many diffs) | |
| 25 | human | 337873 | H2BC20P | H2B clustered histone 20, p... | NR_036461.1 | 61.9% | (many diffs) | |
| 26 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160915.1 | 22.1% | (many diffs) | |
| 27 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_027337.2 | 19.1% | (many diffs) | |
| 28 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160941.1 | 17.8% | (many diffs) | |
| 29 | human | 337875 | H2BP1 | H2B histone pseudogene 1 | NR_160942.1 | 16.5% | (many diffs) | |
| 30 | human | 106479023 | H3P4 | H3 histone pseudogene 4 | NR_160940.1 | 15.9% | (many diffs) | |
| 31 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160913.1 | 14.4% | (many diffs) | |
| 32 | human | 338391 | H2BP2 | H2B histone pseudogene 2 | NR_160914.1 | 11.8% | (many diffs) | |
| 33 | mouse | 319187 | Hist1h2bn | histone cluster 1, H2bn | NM_178201.2 | 92.5% | 98.4% | (many diffs) |
| 34 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001177653.1 | 92.3% | 99.2% | (many diffs) |
| 35 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_001290530.1 | 92.3% | 99.2% | (many diffs) |
| 36 | mouse | 319179 | Hist1h2be | histone cluster 1, H2be | NM_178194.4 | 92.3% | 99.2% | (many diffs) |
| 37 | mouse | 319185 | Hist1h2bl | histone cluster 1, H2bl | NM_178199.2 | 92.3% | 98.4% | (many diffs) |
| 38 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_001290380.1 | 92% | 99.2% | (many diffs) |
| 39 | mouse | 68024 | Hist1h2bc | histone cluster 1, H2bc | NM_023422.3 | 92% | 99.2% | (many diffs) |
| 40 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001313878.1 | 92% | 98.4% | (many diffs) |
| 41 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001313880.1 | 92% | 98.4% | (many diffs) |
| 42 | mouse | 319180 | Hist1h2bf | histone cluster 1, H2bf | NM_178195.2 | 92% | 98.4% | (many diffs) |
| 43 | mouse | 319181 | Hist1h2bg | histone cluster 1, H2bg | NM_178196.4 | 91.7% | 99.2% | (many diffs) |
| 44 | mouse | 319182 | Hist1h2bh | histone cluster 1, H2bh | NM_178197.2 | 91.7% | 98.4% | (many diffs) |
| 45 | mouse | 319186 | Hist1h2bm | histone cluster 1, H2bm | NM_178200.2 | 91.7% | 98.4% | (many diffs) |
| 46 | mouse | 319183 | Hist1h2bj | histone cluster 1, H2bj | NM_178198.2 | 91.5% | 98.4% | (many diffs) |
| 47 | mouse | 319184 | Hist1h2bk | histone cluster 1, H2bk | NM_175665.2 | 91.5% | 97.6% | (many diffs) |
| 48 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_001290466.1 | 91% | 96.8% | (many diffs) |
| 49 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | XM_006516690.2 | 91% | 96.8% | (many diffs) |
| 50 | mouse | 319178 | Hist1h2bb | histone cluster 1, H2bb | NM_175664.3 | 89.4% | 96% | (many diffs) |
| 51 | mouse | 319190 | Hist2h2be | histone cluster 2, H2be | NM_178214.4 | 88.3% | 96.8% | (many diffs) |
| 52 | mouse | 78303 | Hist3h2ba | histone cluster 3, H2ba | NM_030082.4 | 88.2% | 94.4% | (many diffs) |
| 53 | mouse | 319189 | Hist2h2bb | histone cluster 2, H2bb | NM_175666.2 | 87.3% | 97.6% | (many diffs) |
| 54 | mouse | 665596 | Hist1h2bq | histone cluster 1, H2bq | NM_001097979.2 | 86.3% | 91.7% | (many diffs) |
| 55 | mouse | 665622 | Hist1h2br | histone cluster 1 H2br | NM_001110555.1 | 86.3% | 91.7% | (many diffs) |
| 56 | mouse | 319177 | Hist1h2ba | histone cluster 1, H2ba | NM_175663.2 | 82.9% | 88.1% | (many diffs) |
| 57 | mouse | 319188 | Hist1h2bp | histone cluster 1, H2bp | NM_178202.2 | 82.8% | 88.4% | (many diffs) |
| 58 | mouse | 382522 | Hist3h2bb-ps | histone cluster 3, H2bb, ps... | NR_134309.1 | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 468
- ORF length:
- 378
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCTGAACC GGCAAAATCC GCTCCGGCCC 121 CTAAAAAGGG CTCCAAGAAA GCCGTCACCA AAGCCCAGAA GAAAGACGGC AAGAAGCGCA 181 AGCGCAGCCG CAAAGAGAGC TACTCCATCT ACGTGTACAA GGTGCTGAAG CAGGTCCACC 241 CCGACACCGG CATCTCGTCC AAGGCCATGG GCATCATGAA CTCCTTCGTC AACGACATCT 301 TCGAGCGCAT CGCGGGAGAG GCTTCCCGCC TGGCGCACTA CAACAAGCGC TCCACCATCA 361 CATCCCGCGA GATCCAGACG GCCGTGCGCC TGCTGCTGCC CGGCGAGCTG GCCAAGCACG 421 CCGTGTCCGA GGGCACCAAG GCGGTCACCA AGTACACCAG CTCCAAGTGC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAACTACAG CTCACCGGCG TAGGAACACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt