Construct: ORF TRCN0000474612

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002167.1_s317c1
Derived from:
ccsbBroadEn_07321
DNA Barcode:
TGACTTAAAACTTTCTAAGGACCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SQSTM1 (8878)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474612
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8878 SQSTM1 sequestosome 1 NM_001142298.2 99.8% 100% 624C>T;684G>A
2 human 8878 SQSTM1 sequestosome 1 NM_001142299.2 99.8% 100% 624C>T;684G>A
3 human 8878 SQSTM1 sequestosome 1 NM_003900.5 80.7% 80.9% 1_252del;876C>T;936G>A
4 mouse 18412 Sqstm1 sequestosome 1 NM_011018.3 69.5% 73.3% (many diffs)
5 mouse 18412 Sqstm1 sequestosome 1 NM_001290769.1 61.5% 64.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1134
ORF length:
1068
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc catgtcctac gtgaaggatg acatcttccg aatctacatt aaagagaaaa
121aagagtgccg gcgggaccac cgcccaccgt gtgctcagga ggcgccccgc aacatggtgc
181accccaatgt gatctgcgat ggctgcaatg ggcctgtggt aggaacccgc tacaagtgca
241gcgtctgccc agactacgac ttgtgtagcg tctgcgaggg aaagggcttg caccgggggc
301acaccaagct cgcattcccc agccccttcg ggcacctgtc tgagggcttc tcgcacagcc
361gctggctccg gaaggtgaaa cacggacact tcgggtggcc aggatgggaa atgggtccac
421caggaaactg gagcccacgt cctcctcgtg caggggaggc ccgccctggc cccacggcag
481aatcagcttc tggtccatcg gaggatccga gtgtgaattt cctgaagaac gttggggaga
541gtgtggcagc tgcccttagc cctctgggca ttgaagttga tatcgatgtg gagcacggag
601ggaaaagaag ccgccTGACC CCCGTCTCTC CAGAGAGTTC CAGCACAGAG GAGAAGAGCA
661GCTCACAGCC AAGCAGCTGC TGCTCTGATC CCAGCAAGCC GGGTGGGAAT GTTGAGGGCG
721CCACGCAGTC TCTGGCGGAG CAGATGAGAA AGATCGCCTT GGAGTCCGAG GGGCGCCCTG
781AGGAACAGAT GGAGTCGGAT AACTGTTCAG GAGGAGATGA TGACTGGACC CATCTGTCTT
841CAAAAGAAGT GGACCCGTCT ACAGGTGAAC TCCAGTCCCT ACAGATGCCA GAATCCGAAG
901GGCCAAGCTC TCTGGACCCC TCCCAGGAGG GACCCACAGG GCTGAAGGAA GCTGCCTTGT
961ACCCACATCT CCCGCCAGAG GCTGACCCGC GGCTGATTGA GTCCCTCTCC CAGATGCTGT
1021CCATGGGCTT CTCTGATGAA GGCGGCTGGC TCACCAGGCT CCTGCAGACC AAGAACTATG
1081ACATCGGAGC GGCTCTGGAC ACCATCCAGT ATTCAAAGCA TCCCCCGCCG TTGTGCCCAA
1141CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1201ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1261TATCTTGTGG AAAGGACGAT GACTTAAAAC TTTCTAAGGA CCAACGCGTT AAGTCgacaa
1321tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)