Construct: ORF TRCN0000474612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002167.1_s317c1
- Derived from:
- ccsbBroadEn_07321
- DNA Barcode:
- TGACTTAAAACTTTCTAAGGACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SQSTM1 (8878)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8878 | SQSTM1 | sequestosome 1 | NM_001142298.2 | 99.8% | 100% | 624C>T;684G>A |
| 2 | human | 8878 | SQSTM1 | sequestosome 1 | NM_001142299.2 | 99.8% | 100% | 624C>T;684G>A |
| 3 | human | 8878 | SQSTM1 | sequestosome 1 | NM_003900.5 | 80.7% | 80.9% | 1_252del;876C>T;936G>A |
| 4 | mouse | 18412 | Sqstm1 | sequestosome 1 | NM_011018.3 | 69.5% | 73.3% | (many diffs) |
| 5 | mouse | 18412 | Sqstm1 | sequestosome 1 | NM_001290769.1 | 61.5% | 64.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catgtcctac gtgaaggatg acatcttccg aatctacatt aaagagaaaa 121 aagagtgccg gcgggaccac cgcccaccgt gtgctcagga ggcgccccgc aacatggtgc 181 accccaatgt gatctgcgat ggctgcaatg ggcctgtggt aggaacccgc tacaagtgca 241 gcgtctgccc agactacgac ttgtgtagcg tctgcgaggg aaagggcttg caccgggggc 301 acaccaagct cgcattcccc agccccttcg ggcacctgtc tgagggcttc tcgcacagcc 361 gctggctccg gaaggtgaaa cacggacact tcgggtggcc aggatgggaa atgggtccac 421 caggaaactg gagcccacgt cctcctcgtg caggggaggc ccgccctggc cccacggcag 481 aatcagcttc tggtccatcg gaggatccga gtgtgaattt cctgaagaac gttggggaga 541 gtgtggcagc tgcccttagc cctctgggca ttgaagttga tatcgatgtg gagcacggag 601 ggaaaagaag ccgccTGACC CCCGTCTCTC CAGAGAGTTC CAGCACAGAG GAGAAGAGCA 661 GCTCACAGCC AAGCAGCTGC TGCTCTGATC CCAGCAAGCC GGGTGGGAAT GTTGAGGGCG 721 CCACGCAGTC TCTGGCGGAG CAGATGAGAA AGATCGCCTT GGAGTCCGAG GGGCGCCCTG 781 AGGAACAGAT GGAGTCGGAT AACTGTTCAG GAGGAGATGA TGACTGGACC CATCTGTCTT 841 CAAAAGAAGT GGACCCGTCT ACAGGTGAAC TCCAGTCCCT ACAGATGCCA GAATCCGAAG 901 GGCCAAGCTC TCTGGACCCC TCCCAGGAGG GACCCACAGG GCTGAAGGAA GCTGCCTTGT 961 ACCCACATCT CCCGCCAGAG GCTGACCCGC GGCTGATTGA GTCCCTCTCC CAGATGCTGT 1021 CCATGGGCTT CTCTGATGAA GGCGGCTGGC TCACCAGGCT CCTGCAGACC AAGAACTATG 1081 ACATCGGAGC GGCTCTGGAC ACCATCCAGT ATTCAAAGCA TCCCCCGCCG TTGTGCCCAA 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAT GACTTAAAAC TTTCTAAGGA CCAACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att