Construct: ORF TRCN0000474629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006086.1_s317c1
- Derived from:
- ccsbBroadEn_09083
- DNA Barcode:
- AAAGTTACACAATAAAACGGCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R14C (81706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81706 | PPP1R14C | protein phosphatase 1 regul... | NM_030949.3 | 99.5% | 99.3% | 12G>A;28A>G |
| 2 | human | 81706 | PPP1R14C | protein phosphatase 1 regul... | XM_005267152.5 | 54.5% | 44.8% | (many diffs) |
| 3 | mouse | 76142 | Ppp1r14c | protein phosphatase 1, regu... | NM_133485.2 | 87.4% | 91.5% | (many diffs) |
| 4 | mouse | 76142 | Ppp1r14c | protein phosphatase 1, regu... | XM_006512489.2 | 78.8% | 82.5% | (many diffs) |
| 5 | mouse | 76142 | Ppp1r14c | protein phosphatase 1, regu... | XM_006512490.3 | 67.7% | 70.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC GGTGGCAACG GGCAGCAGCG AGGCGGCCGG CGGGGCCAGC GGCGGCGGCG 121 CACGGGTTTT CTTCCAAAGC CCCCGGGGTG GCGCCGGTGG CAGCCCCGGC TCCAGCAGCG 181 GCTCAGGCTC CTCCCGGGAG GACTCGGCGC CCGTGGCCAC GGCGGCCGCT GCAGGGCAGG 241 TTCAGCAGCA ACAGCAGCGG CGACACCAGC AGGGAAAAGT GACAGTGAAA TACGATCGTA 301 AGGAGCTTCG GAAGCGGCTG GTGCTGGAGG AATGGATCGT GGAGCAGCTG GGTCAGCTCT 361 ACGGCTGCGA GGAAGAAGAA ATGCCAGAGG TAGAAATTGA CATTGATGAT CTTCTTGATG 421 CAGACAGTGA TGAAGAGAGA GCTTCAAAAT TACAGGAAGC TCTTGTAGAC TGCTACAAAC 481 CAACAGAGGA ATTTATCAAA GAGCTGCTTT CTCGGATAAG AGGCATGAGG AAACTGAGCC 541 CTCCGCAGAA GAAGAGTGTA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAAAG TTACACAATA 721 AAACGGCTCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt