Construct: ORF TRCN0000474634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006887.1_s317c1
- Derived from:
- ccsbBroadEn_14028
- DNA Barcode:
- TCTAACACTGCAATAGATTCCCGC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATG13 (9776)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9776 | ATG13 | autophagy related 13 | NM_001205121.1 | 99.9% | 99.3% | 1430delA |
| 2 | human | 9776 | ATG13 | autophagy related 13 | NM_001346342.1 | 99.9% | 99.3% | 1430delA |
| 3 | human | 9776 | ATG13 | autophagy related 13 | NM_001346344.1 | 99.9% | 99.3% | 1430delA |
| 4 | human | 9776 | ATG13 | autophagy related 13 | NM_001346346.1 | 99.9% | 99.3% | 1430delA |
| 5 | human | 9776 | ATG13 | autophagy related 13 | NM_001346348.1 | 99.9% | 99.3% | 1430delA |
| 6 | human | 9776 | ATG13 | autophagy related 13 | NM_001346349.1 | 99.9% | 99.3% | 1430delA |
| 7 | human | 9776 | ATG13 | autophagy related 13 | NM_001346350.1 | 99.9% | 99.3% | 1430delA |
| 8 | human | 9776 | ATG13 | autophagy related 13 | NM_001346351.1 | 99.9% | 99.3% | 1430delA |
| 9 | human | 9776 | ATG13 | autophagy related 13 | NM_001346352.1 | 99.9% | 99.3% | 1430delA |
| 10 | human | 9776 | ATG13 | autophagy related 13 | NM_001346353.1 | 99.9% | 99.3% | 1430delA |
| 11 | human | 9776 | ATG13 | autophagy related 13 | NM_001346354.1 | 99.9% | 99.3% | 1430delA |
| 12 | human | 9776 | ATG13 | autophagy related 13 | NM_014741.4 | 99.9% | 99.3% | 1430delA |
| 13 | human | 9776 | ATG13 | autophagy related 13 | NM_001346355.1 | 97% | 96.4% | 1118_1119ins42;1388delA |
| 14 | human | 9776 | ATG13 | autophagy related 13 | NM_001346356.1 | 97% | 96.4% | 1118_1119ins42;1388delA |
| 15 | human | 9776 | ATG13 | autophagy related 13 | NM_001346333.1 | 94.4% | 93.8% | 786_869del;1514delA |
| 16 | human | 9776 | ATG13 | autophagy related 13 | NM_001346334.1 | 94.4% | 93.8% | 786_869del;1514delA |
| 17 | human | 9776 | ATG13 | autophagy related 13 | NM_001346335.1 | 94.4% | 93.8% | 786_869del;1514delA |
| 18 | human | 9776 | ATG13 | autophagy related 13 | NM_001346336.1 | 94.4% | 93.8% | 786_869del;1514delA |
| 19 | human | 9776 | ATG13 | autophagy related 13 | NM_001346337.1 | 94.4% | 93.8% | 786_869del;1514delA |
| 20 | human | 9776 | ATG13 | autophagy related 13 | XM_011520502.2 | 94.4% | 93.8% | 786_869del;1514delA |
| 21 | human | 9776 | ATG13 | autophagy related 13 | NM_001142673.2 | 92.7% | 92.2% | 786_896del;1541delA |
| 22 | human | 9776 | ATG13 | autophagy related 13 | NM_001205120.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 23 | human | 9776 | ATG13 | autophagy related 13 | NM_001346319.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 24 | human | 9776 | ATG13 | autophagy related 13 | NM_001346320.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 25 | human | 9776 | ATG13 | autophagy related 13 | NM_001346321.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 26 | human | 9776 | ATG13 | autophagy related 13 | NM_001346322.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 27 | human | 9776 | ATG13 | autophagy related 13 | NM_001346323.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 28 | human | 9776 | ATG13 | autophagy related 13 | NM_001346324.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 29 | human | 9776 | ATG13 | autophagy related 13 | NM_001346325.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 30 | human | 9776 | ATG13 | autophagy related 13 | NM_001346326.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 31 | human | 9776 | ATG13 | autophagy related 13 | NM_001346327.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 32 | human | 9776 | ATG13 | autophagy related 13 | NM_001346328.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 33 | human | 9776 | ATG13 | autophagy related 13 | NM_001346329.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 34 | human | 9776 | ATG13 | autophagy related 13 | NM_001346330.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 35 | human | 9776 | ATG13 | autophagy related 13 | NM_001346331.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 36 | human | 9776 | ATG13 | autophagy related 13 | NM_001346332.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 37 | human | 9776 | ATG13 | autophagy related 13 | XM_017018603.2 | 92.7% | 92.2% | 786_896del;1541delA |
| 38 | human | 9776 | ATG13 | autophagy related 13 | XM_017018607.1 | 92.7% | 92.2% | 786_896del;1541delA |
| 39 | human | 9776 | ATG13 | autophagy related 13 | NM_001346338.1 | 90% | 89.5% | 786_896del;1229_1230ins42;1499delA |
| 40 | human | 9776 | ATG13 | autophagy related 13 | NM_001346340.1 | 90% | 89.5% | 786_896del;1229_1230ins42;1499delA |
| 41 | human | 9776 | ATG13 | autophagy related 13 | NM_001346317.1 | 88.6% | 88.1% | 786_968del;1613delA |
| 42 | human | 9776 | ATG13 | autophagy related 13 | NM_001346318.1 | 88.6% | 88.1% | 786_968del;1613delA |
| 43 | human | 9776 | ATG13 | autophagy related 13 | XM_011520500.2 | 88.6% | 88.1% | 786_968del;1613delA |
| 44 | human | 9776 | ATG13 | autophagy related 13 | NM_001205119.1 | 87.2% | 86.7% | 786_995del;1640delA |
| 45 | human | 9776 | ATG13 | autophagy related 13 | NM_001346311.1 | 87.2% | 86.7% | 786_995del;1640delA |
| 46 | human | 9776 | ATG13 | autophagy related 13 | NM_001346312.1 | 87.2% | 86.7% | 786_995del;1640delA |
| 47 | human | 9776 | ATG13 | autophagy related 13 | NM_001346313.1 | 87.2% | 86.7% | 786_995del;1640delA |
| 48 | human | 9776 | ATG13 | autophagy related 13 | NM_001346314.1 | 87.2% | 86.7% | 786_995del;1640delA |
| 49 | human | 9776 | ATG13 | autophagy related 13 | NM_001346315.1 | 87.2% | 86.7% | 786_995del;1640delA |
| 50 | human | 9776 | ATG13 | autophagy related 13 | NM_001346316.1 | 87.2% | 86.7% | 786_995del;1640delA |
| 51 | human | 9776 | ATG13 | autophagy related 13 | XM_005253265.3 | 87.2% | 86.7% | 786_995del;1640delA |
| 52 | human | 9776 | ATG13 | autophagy related 13 | XM_005253266.3 | 87.2% | 86.7% | 786_995del;1640delA |
| 53 | human | 9776 | ATG13 | autophagy related 13 | XM_005253268.2 | 87.2% | 86.7% | 786_995del;1640delA |
| 54 | human | 9776 | ATG13 | autophagy related 13 | XM_006718396.3 | 87.2% | 86.7% | 786_995del;1640delA |
| 55 | human | 9776 | ATG13 | autophagy related 13 | XM_011520493.2 | 87.2% | 86.7% | 786_995del;1640delA |
| 56 | human | 9776 | ATG13 | autophagy related 13 | XM_011520496.3 | 87.2% | 86.7% | 786_995del;1640delA |
| 57 | human | 9776 | ATG13 | autophagy related 13 | XM_011520499.3 | 87.2% | 86.7% | 786_995del;1640delA |
| 58 | human | 9776 | ATG13 | autophagy related 13 | XM_017018599.2 | 87.2% | 86.7% | 786_995del;1640delA |
| 59 | human | 9776 | ATG13 | autophagy related 13 | NM_001205122.1 | 83.4% | 82.9% | 0_1ins237;1193delA |
| 60 | human | 9776 | ATG13 | autophagy related 13 | NM_001346360.1 | 59.3% | 58.7% | 0_1ins585;845delA |
| 61 | human | 9776 | ATG13 | autophagy related 13 | XM_024448781.1 | 59.3% | 58.7% | 0_1ins585;845delA |
| 62 | human | 9776 | ATG13 | autophagy related 13 | NM_001346357.1 | 55% | 54.5% | 0_1ins585;201_311del;956delA |
| 63 | human | 9776 | ATG13 | autophagy related 13 | NM_001346358.1 | 55% | 54.5% | 0_1ins585;201_311del;956delA |
| 64 | human | 9776 | ATG13 | autophagy related 13 | NM_001346359.1 | 55% | 54.5% | 0_1ins585;201_311del;956delA |
| 65 | human | 9776 | ATG13 | autophagy related 13 | XM_017018618.2 | 51.7% | 51.2% | 0_1ins585;201_410del;1055delA |
| 66 | human | 9776 | ATG13 | autophagy related 13 | NR_144422.1 | 23.8% | (many diffs) | |
| 67 | human | 9776 | ATG13 | autophagy related 13 | NR_144423.1 | 23.5% | (many diffs) | |
| 68 | human | 9776 | ATG13 | autophagy related 13 | NR_144424.1 | 22.3% | (many diffs) | |
| 69 | mouse | 51897 | Atg13 | autophagy related 13 | NM_145528.3 | 88.7% | 93.5% | (many diffs) |
| 70 | mouse | 51897 | Atg13 | autophagy related 13 | XM_006499892.2 | 83.9% | 88.5% | (many diffs) |
| 71 | mouse | 51897 | Atg13 | autophagy related 13 | XM_006499891.3 | 83.8% | 88.3% | (many diffs) |
| 72 | mouse | 51897 | Atg13 | autophagy related 13 | XM_006499890.3 | 82.4% | 86.8% | (many diffs) |
| 73 | mouse | 51897 | Atg13 | autophagy related 13 | XM_006499888.2 | 78.7% | 82.9% | (many diffs) |
| 74 | mouse | 51897 | Atg13 | autophagy related 13 | XM_017319122.1 | 77.9% | 82.2% | (many diffs) |
| 75 | mouse | 51897 | Atg13 | autophagy related 13 | XM_006499887.2 | 77.4% | 81.6% | (many diffs) |
| 76 | mouse | 51897 | Atg13 | autophagy related 13 | XM_006499889.3 | 73.2% | 77.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggaatgga aactgatctc aattcccagg acagaaagga cctggacaag tttattaaat 121 tttttgccct caagactgtc caagtgattg tccaggctcg gcttggtgaa aagatttgca 181 ctcgttcatc atcttctcca acgggttcag attggttcaa cttagcaatc aaagacatcc 241 cagaggttac acatgaagca aagaaggcac tggcaggaca gctgcctgca gtcgggaggt 301 ccatgtgtgt ggagatttca cttaagactt ctgagggaga ttccatggag ctggaaatat 361 ggtgtcttga aatgaatgaa aagtgtgata aagaaatcaa agtttcctac acggtgtaca 421 acagactgtc attgctgctg aagtcccttc ttgctataac tagggtgaca ccagcctata 481 ggctctccag gaaacaaggg catgaatatg tcatattata caggatatat tttggagaag 541 ttcagctgag tggcttagga gaaggcttcc agacagttcg tgttgggaca gtgggcaccc 601 ctgtgggcac catcactctt tcttgtgctt acagaattaa cttggcattc atgtctacca 661 ggcaatttga gaggacccca cctatcatgg ggattattat tgatcacttt gtggaccgtc 721 cctatcccag ctcctctccc atgcacccct gcaattacag aactgctggt gaggacactg 781 gagtaatata cccgtctgta gaagactctc aagaagtgtg taccacctct ttttccacct 841 ccccaccatc ccagctgatg gttcctggga aggaaggtgg ggtacccctt gctcccaacc 901 agcctgtcca tggtacccag gctgaccagg agagactggc aacctgcacc ccttctgaca 961 gaacccactg tgctgccaca cccTCCAGTA GTGAGGATAC TGAAACCGTA TCAAACAGCA 1021 GTGAGGGACG GGCCTCCCCT CACGATGTCT TGGAGACCAT CTTTGTCCGA AAAGTGGGGG 1081 CTTTTGTCAA CAAACCCATT AACCAGGTGA CCCTGACGAG TTTGGATATA CCCTTTGCCA 1141 TGTTTGCTCC CAAGAATTTG GAGCTGGAGG ATACCGATCC AATGGTGAAT CCTCCAGATT 1201 CCCCAGAGAC TGAATCTCCT CTCCAGGGCA GCCTGCACTC AGATGGCTCC AGCGGGGGCA 1261 GCAGTGGCAA TACCCATGAT GACTTTGTTA TGATAGACTT TAAACCAGCT TTTTCTAAAG 1321 ATGACATTCT TCCGATGGAC CTGGGGACCT TCTATCGGGA GTTTCAGAAC CCACCTCAGC 1381 TGAGCAGCCT CTCCATAGAT ATTGGAGCAC AGTCCATGGC TGAAGACTTG GACTCATTAC 1441 CAGAGAAGCT GGCTGTGCAT GAGAAGAATG TCCGCGAGTT TGATGCCTTT GTGGAACCCT 1501 GCAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 1561 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 1621 TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCTAACACTG CAATAGATTC CCGCACGCGT 1681 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt