Transcript: Human NM_001346358.1

Homo sapiens autophagy related 13 (ATG13), transcript variant 49, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
ATG13 (9776)
Length:
6006
CDS:
1353..2321

Additional Resources:

NCBI RefSeq record:
NM_001346358.1
NBCI Gene record:
ATG13 (9776)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001346358.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000415190 GGACCTTCTATCGGGAGTTTC pLKO_005 2158 CDS 100% 10.800 15.120 N ATG13 n/a
2 TRCN0000167734 GACCTGGACAAGTTTATTAAA pLKO.1 662 5UTR 100% 15.000 10.500 N ATG13 n/a
3 TRCN0000423616 TGAAGTCCCTTCTTGCTATAA pLKO_005 1002 5UTR 100% 13.200 9.240 N ATG13 n/a
4 TRCN0000172801 GCCATGTTTGCTCCCAAGAAT pLKO.1 1950 CDS 100% 5.625 3.938 N ATG13 n/a
5 TRCN0000172507 CCCACCTATGATCTGTCCTTT pLKO.1 3723 3UTR 100% 4.950 3.465 N ATG13 n/a
6 TRCN0000427008 GACCCAGAAGTCCCTACTCTT pLKO_005 2453 3UTR 100% 4.950 3.465 N ATG13 n/a
7 TRCN0000172704 GAGAAGAATGTCCGCGAGTTT pLKO.1 2274 CDS 100% 4.950 3.465 N ATG13 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001346358.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14028 pDONR223 100% 55% 54.5% None 0_1ins585;201_311del;956delA n/a
2 ccsbBroad304_14028 pLX_304 0% 55% 54.5% V5 (not translated due to frame shift) 0_1ins585;201_311del;956delA n/a
3 TRCN0000474634 TCTAACACTGCAATAGATTCCCGC pLX_317 36.4% 55% 54.5% V5 (not translated due to frame shift) 0_1ins585;201_311del;956delA n/a
4 ccsbBroadEn_15678 pDONR223 0% 39.4% 37.2% None (many diffs) n/a
5 ccsbBroad304_15678 pLX_304 0% 39.4% 37.2% V5 (many diffs) n/a
6 TRCN0000471230 TTAAGTCGTCACACAAGTTTCACA pLX_317 35.8% 39.4% 37.2% V5 (many diffs) n/a
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