Construct: ORF TRCN0000474635
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011853.1_s317c1
- Derived from:
- ccsbBroadEn_12328
- DNA Barcode:
- CCTCCCTCAACGAGCTCCTTGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARP11 (57097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474635
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57097 | PARP11 | poly(ADP-ribose) polymerase... | XM_011520970.2 | 100% | 100% | |
| 2 | human | 57097 | PARP11 | poly(ADP-ribose) polymerase... | XM_011520973.2 | 100% | 100% | |
| 3 | human | 57097 | PARP11 | poly(ADP-ribose) polymerase... | NM_020367.6 | 97.9% | 97.9% | 1_21del |
| 4 | human | 57097 | PARP11 | poly(ADP-ribose) polymerase... | NM_001286521.1 | 77.6% | 75% | 0_1ins101;22_23ins121 |
| 5 | human | 57097 | PARP11 | poly(ADP-ribose) polymerase... | NM_001286522.2 | 77.6% | 75% | 0_1ins101;22_23ins121 |
| 6 | human | 57097 | PARP11 | poly(ADP-ribose) polymerase... | XM_017019671.1 | 77.6% | 75% | 0_1ins101;22_23ins121 |
| 7 | human | 57097 | PARP11 | poly(ADP-ribose) polymerase... | NR_104461.2 | 22% | 1_191del;718_776del;1244_4508del | |
| 8 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | NM_181402.3 | 88.4% | 94.8% | (many diffs) |
| 9 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_006505231.3 | 88.4% | 94.8% | (many diffs) |
| 10 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_006505232.3 | 88.4% | 94.8% | (many diffs) |
| 11 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_006505233.3 | 88.4% | 94.8% | (many diffs) |
| 12 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_017321285.1 | 86.5% | 92.8% | (many diffs) |
| 13 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_006505234.3 | 68.5% | 72.2% | (many diffs) |
| 14 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_006505235.3 | 68.5% | 72.2% | (many diffs) |
| 15 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | NM_001346512.1 | 65.8% | 72.2% | (many diffs) |
| 16 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | NM_001346513.1 | 65.8% | 72.2% | (many diffs) |
| 17 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_006505237.2 | 57.3% | 62.2% | (many diffs) |
| 18 | mouse | 101187 | Parp11 | poly (ADP-ribose) polymeras... | XM_017321286.1 | 48.4% | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcacaaagca gaagaattat tttctaaaac aacaaacaat gaagtggatg 121 acatggacac gtcagatacc cagtggggct ggttttactt ggcagaatgt gggaagtggc 181 acatgtttca gccggatacc aacagtcagt gttcagttag cagtgaagat atcgaaaaaa 241 gcttcaaaac aaacccttgt ggctccattt cttttactac ttccaaattc agctacaaga 301 tagactttgc agaaatgaag caaatgaatc tcaccactgg aaagcagcgc ttaataaaaa 361 gagccccctt ttctatcagt gctttcagtt acatctgtga aaacgaggcc atccctatgc 421 caccacactg ggagaatgtg aatactcaag taccatatca gcttattcct ctgcacaatc 481 aaacacatga atataatgaa gttgctaatc tctttgggaa gacgatggat cGCAACCGAA 541 TTAAAAGAAT TCAGAGAATT CAAAACCTAG ATTTGTGGGA GTTCTTTTGC AGGAAAAAGG 601 CTCAGCTCAA GAAAAAAAGA GGTGTGCCTC AGATTAATGA ACAAATGCTG TTTCATGGTA 661 CCAGCAGTGA ATTTGTGGAA GCAATCTGCA TTCATAACTT TGATTGGAGA ATAAATGGTA 721 TACATGGTGC TGTCTTTGGA AAAGGAACCT ATTTTGCTAG AGATGCTGCT TATTCCAGTC 781 GTTTCTGCAA AGATGACATA AAGCATGGGA ACACATTCCA AATTCATGGT GTCAGCTTGC 841 AACAGCGGCA TCTGTTTAGA ACATATAAAT CTATGTTTCT TGCTCGAGTG CTAATTGGAG 901 ATTACATAAA CGGAGACTCC AAATACATGC GACCTCCTTC CAAAGACGGG AGCTATGTGA 961 ATTTATATGA CAGCTGTGTG GATGATACCT GGAACCCAAA GATCTTTGTG GTTTTTGATG 1021 CCAACCAAAT CTATCCTGAG TACTTGATAG ACTTTCATTG CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGACCTCCC TCAACGAGCT CCTTGCTGAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt