Construct: ORF TRCN0000474698
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014796.1_s317c1
- Derived from:
- ccsbBroadEn_03100
- DNA Barcode:
- TTGTATGGGTTAGAATCATCGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST8SIA5 (29906)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474698
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29906 | ST8SIA5 | ST8 alpha-N-acetyl-neuramin... | NM_013305.6 | 100% | 100% | |
| 2 | human | 29906 | ST8SIA5 | ST8 alpha-N-acetyl-neuramin... | NM_001307987.2 | 91.7% | 91.7% | 131_132ins93 |
| 3 | human | 29906 | ST8SIA5 | ST8 alpha-N-acetyl-neuramin... | NM_001307986.2 | 91.2% | 91.2% | 130_237del |
| 4 | human | 29906 | ST8SIA5 | ST8 alpha-N-acetyl-neuramin... | XM_017025715.1 | 80.3% | 80.3% | 0_1ins222 |
| 5 | human | 29906 | ST8SIA5 | ST8 alpha-N-acetyl-neuramin... | XM_017025716.1 | 60.6% | 60.6% | 0_1ins444 |
| 6 | mouse | 225742 | St8sia5 | ST8 alpha-N-acetyl-neuramin... | NM_153124.2 | 85.6% | 85.1% | (many diffs) |
| 7 | mouse | 225742 | St8sia5 | ST8 alpha-N-acetyl-neuramin... | XM_006526430.3 | 85.4% | 84.6% | (many diffs) |
| 8 | mouse | 225742 | St8sia5 | ST8 alpha-N-acetyl-neuramin... | NM_013666.2 | 78.1% | 77.6% | (many diffs) |
| 9 | mouse | 225742 | St8sia5 | ST8 alpha-N-acetyl-neuramin... | XM_006526429.3 | 77.9% | 77.2% | (many diffs) |
| 10 | mouse | 225742 | St8sia5 | ST8 alpha-N-acetyl-neuramin... | XM_006526431.3 | 51.9% | 51.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1197
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgctacgcg gacccctcgg ccaaccggga tttgttgggg agccgaactt 121 tgctcttcat cttcatctgc gcctttgcct tggtgacctt gctgcaacag atcctgtatg 181 gcaggaacta cattaagagg tactttgaat tttatgaggg gccttttgaa tataactcca 241 caagatgcct ggagctgagg cacgaaatat tggaagtgaa ggtgctgtcc atggtgaagc 301 agtcagagct gttcgacagg tggaagagcc tccagatgtg caaatgggcg atgaacatct 361 ctgaggccaa ccagttcaag tctactctgt ccaggtgctg caacgcccct gcctttctct 421 tcaccaccca gaagaacact cccctgggga caaagctcaa gtatgaggtg gacaccagtg 481 gcatctacca catcaaccag gagatcttcc gcatgtttcc caaggacatg ccctactacc 541 ggtcccagtt taagaagtgt gctgtagtgg gcaacggagg catcttgaag aacagccgct 601 gcgggaggga gatcaacagc gccgacttcg tcttccggtg caacctgccc cccatctcag 661 agaagtacac catGGATGTG GGGGTGAAGA CGGATGTGGT CACTGTGAAC CCCAGCATCA 721 TCACAGAGAG GTTCCACAAG CTGGAGAAGT GGCGGCGGCC GTTCTATCGC GTGCTGCAGG 781 TGTACGAGAA CGCGTCGGTG CTGCTGCCTG CCTTCTACAA CACGCGCAAC ACCGACGTGT 841 CCATCCGCGT CAAGTACGTG CTGGACGACT TCGAATCGCC GCAAGCTGTC TACTACTTCC 901 ATCCGCAGTA CCTGGTCAAC GTGTCGCGCT ACTGGCTCAG CCTGGGGGTG CGCGCCAAGC 961 GCATCAGCAC CGGCCTCATT CTGGTCACTG CGGCGCTGGA GCTCTGTGAG GAGGTGCACC 1021 TCTTTGGCTT CTGGGCCTTC CCCATGAACC CCTCGGGCCT CTACATCACT CACCACTACT 1081 ATGACAACGT CAAGCCGCGT CCCGGCTTCC ACGCCATGCC CTCTGAGATC TTCAACTTCC 1141 TGCACTTGCA CAGCCGAGGC ATCCTCCGCG TGCACACGGG CACCTGCAGC TGCTGCTTGC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GATTGTATGG GTTAGAATCA TCGGTCACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt