Transcript: Mouse NM_153124.2

Mus musculus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
St8sia5 (225742)
Length:
2006
CDS:
249..1379

Additional Resources:

NCBI RefSeq record:
NM_153124.2
NBCI Gene record:
St8sia5 (225742)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_153124.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000110407 CTTCTTCATCACGCACCATTA pLKO.1 1238 CDS 100% 10.800 8.640 N St8sia5 n/a
2 TRCN0000110408 CGTGAATCCCAGCATCATAAT pLKO.1 884 CDS 100% 13.200 9.240 N St8sia5 n/a
3 TRCN0000110409 CCAGATATGCAAATGGGCAAT pLKO.1 512 CDS 100% 4.050 2.835 N St8sia5 n/a
4 TRCN0000110406 GAATCGAACTTTGCTCTTTAT pLKO.1 290 CDS 100% 13.200 7.920 N St8sia5 n/a
5 TRCN0000110405 CCTGAAGGATTCCTTGACCTT pLKO.1 1421 3UTR 100% 2.640 1.584 N St8sia5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153124.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03100 pDONR223 100% 85.6% 85.1% None (many diffs) n/a
2 ccsbBroad304_03100 pLX_304 0% 85.6% 85.1% V5 (many diffs) n/a
3 TRCN0000474698 TTGTATGGGTTAGAATCATCGGTC pLX_317 46.3% 85.6% 85.1% V5 (many diffs) n/a
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