Construct: ORF TRCN0000474721
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013902.1_s317c1
- Derived from:
- ccsbBroadEn_01177
- DNA Barcode:
- GCGCCAGCTAGCTGGGGCACACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PFDN1 (5201)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474721
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5201 | PFDN1 | prefoldin subunit 1 | NM_002622.5 | 100% | 100% | |
| 2 | human | 5201 | PFDN1 | prefoldin subunit 1 | XM_005268465.4 | 80.8% | 81.9% | (many diffs) |
| 3 | mouse | 67199 | Pfdn1 | prefoldin 1 | NM_026027.3 | 91.2% | 97.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 456
- ORF length:
- 366
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCCGCCCC CGTGGATCTA GAGCTGAAGA 121 AGGCCTTCAC AGAGCTTCAA GCCAAAGTTA TTGACACTCA ACAGAAGGTG AAGCTCGCAG 181 ACATACAGAT TGAACAGCTA AACAGAACGA AAAAGCATGC ACATCTTACA GATACAGAGA 241 TCATGACTTT GGTAGATGAG ACTAACATGT ATGAAGGTGT AGGAAGAATG TTTATTCTTC 301 AGTCCAAGGA AGCAATTCAC AGTCAGCTGT TAGAGAAGCA GAAAATAGCA GAAGAAAAAA 361 TTAAAGAACT AGAACAGAAA AAGTCCTACC TGGAGCGAAG CGTTAAGGAA GCTGAGGACA 421 ACATCCGGGA GATGCTGATG GCACGAAGGG CCCAGTACCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AGCGCCAGCT AGCTGGGGCA CACGGACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt