Construct: ORF TRCN0000474730

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015271.1_s317c1
Derived from:
ccsbBroadEn_15421
DNA Barcode:
CCGTCAGAAGAAGATAGTACCATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GABPB1 (2553)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474730
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2553 GABPB1 GA binding protein transcri... NM_016654.5 99.9% 99.7% 784A>G
2 human 2553 GABPB1 GA binding protein transcri... XM_017022053.2 99.9% 99.7% 784A>G
3 human 2553 GABPB1 GA binding protein transcri... XM_011521426.3 99.6% 99.4% 695_696insAGT;781A>G
4 human 2553 GABPB1 GA binding protein transcri... XM_017022054.2 99.6% 99.4% 695_696insAGT;781A>G
5 human 2553 GABPB1 GA binding protein transcri... XM_024449887.1 98.6% 98.4% 1_15del;799A>G
6 human 2553 GABPB1 GA binding protein transcri... XM_024449888.1 98.3% 98.1% 1_15del;710_711insAGT;796A>G
7 human 2553 GABPB1 GA binding protein transcri... NM_001320910.2 96.8% 96.7% 579_614del;820A>G
8 human 2553 GABPB1 GA binding protein transcri... NM_005254.6 96.8% 96.7% 579_614del;820A>G
9 human 2553 GABPB1 GA binding protein transcri... XM_005254274.4 96.8% 96.7% 579_614del;820A>G
10 human 2553 GABPB1 GA binding protein transcri... NM_001320915.2 96.6% 96.4% 579_614del;731_732insAGT;817A>G
11 human 2553 GABPB1 GA binding protein transcri... XM_024449886.1 96.6% 96.4% 579_614del;731_732insAGT;817A>G
12 human 2553 GABPB1 GA binding protein transcri... XM_024449885.1 96.1% 95.9% 1_9delATGTGTAAG;588_623del;829A>G
13 human 2553 GABPB1 GA binding protein transcri... XM_024449883.1 95.6% 95.5% 1_15del;594_629del;835A>G
14 human 2553 GABPB1 GA binding protein transcri... XM_024449884.1 95.4% 95.2% (many diffs)
15 human 2553 GABPB1 GA binding protein transcri... NM_016655.4 88.8% 87.4% (many diffs)
16 human 2553 GABPB1 GA binding protein transcri... NM_181427.3 88.8% 87.4% (many diffs)
17 human 2553 GABPB1 GA binding protein transcri... NM_002041.4 86.1% 84.8% (many diffs)
18 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271468.1 91.5% 97.9% (many diffs)
19 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498757.3 91.5% 97.9% (many diffs)
20 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498758.3 91.5% 97.9% (many diffs)
21 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498759.3 91.5% 97.9% (many diffs)
22 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498760.3 91.5% 97.9% (many diffs)
23 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271467.1 91.2% 97.6% (many diffs)
24 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_207669.2 91.2% 97.6% (many diffs)
25 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498761.3 91.2% 97.6% (many diffs)
26 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498755.1 89.6% 95.9% (many diffs)
27 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498756.1 89.4% 95.6% (many diffs)
28 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_010249.2 82.5% 83.8% (many diffs)
29 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271470.1 82.2% 83.6% (many diffs)
30 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498762.1 79.9% 83.6% (many diffs)
31 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_017315588.1 75.1% 80.9% (many diffs)
32 mouse 14391 Gabpb1 GA repeat binding protein, ... XM_006498764.3 72.2% 74.9% (many diffs)
33 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271492.1 56.7% 60% (many diffs)
34 mouse 14391 Gabpb1 GA repeat binding protein, ... NR_073183.1 49% (many diffs)
35 mouse 14391 Gabpb1 GA repeat binding protein, ... NM_001271469.1 32.8% 36.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1215
ORF length:
1149
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc cctggtagat ttgggaaaga agcttttaga agcggcacga gcaggtcaag
121atgatgaagt tcgtattttg atggcaaatg gagctccctt tactacagac tggctgggaa
181cttctccact tcatctagca gcacagtatg gtcattattc caccacagag gtactgctgc
241gagctggtgt gagcagagat gccagaacca aagtggaccg aacaccatta catatggcag
301cttctgaggg ccatgccagc atagtagagg ttttacttaa gcatggtgct gatgtcaatg
361caaaggacat gttaaagatg acagctctcc attgggccac agaacacaat catcaagagg
421tggtggaact tttaatcaaa tatggtgctg atgtacacac gcaaagtaaa ttttgtaaaa
481ctgcatttga tatttcaata gacaatggaa atgaagattt agcagagata ttacagattg
541ctatgcagaa ccaaatcaac acaaacccag agagtcctga cactgtgaca atacatgctg
601caacaccaca gtttatcatt ggacctggag gggtggtgaa cctaacagat gaaacgggtg
661tatctgctgt tcagtttgga aactcttcta catcagtatt agctacatta gctgccTTAG
721CTGAAGCATC TGCTCCATTG TCCAATTCTT CAGAAACTCC AGTAGTGGCC ACAGAAGAAG
781TAGTTACTGC AGAATCTGTG GATGGTGCCA TTCAGCAAGT AGTTAGTTCA GGGGGTCAGC
841AAGTCATCGC AATAGTTACA GATGGAATTC AGCTTGGAAA TTTGCACTCT ATTCCAACCA
901GTGGAATTGG TCAGCCCATC ATTGTGACCA TGCCAGATGG ACAACAAGTA TTAACAGTAC
961CAGCAACAGA CATTGCTGAA GAAACTGTTA TAAGTGAAGA ACCACCAGCT AAGAGACAAT
1021GTATCGAAAT AATTGAAAAC CGGGTGGAAT CTGCAGAAAT AGAAGAGAGA GAAGCTCTTC
1081AGAAACAGCT GGATGAAGCA AATCGAGAAG CACAAAAATA TCGACAGCAG CTCCTAAAGA
1141AAGAACAGGA AGCAGAGGCC TACAGACAGA AGTTGGAAGC TATGACTCGT CTTCAGACTA
1201ATAAAGAAGC TGTTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1261CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1321TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCGTCAGAAG AAGATAGTAC
1381CATAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)