Construct: ORF TRCN0000474755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010305.1_s317c1
- Derived from:
- ccsbBroadEn_06968
- DNA Barcode:
- GTAAGGTGCCATCCTTACCCCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC34A1 (6569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474755
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6569 | SLC34A1 | solute carrier family 34 me... | NM_003052.5 | 99.9% | 99.8% | 1784G>C |
| 2 | human | 6569 | SLC34A1 | solute carrier family 34 me... | XM_024446191.1 | 91.1% | 90.9% | 1006_1007ins168;1616G>C |
| 3 | human | 6569 | SLC34A1 | solute carrier family 34 me... | XM_017009773.2 | 79.7% | 73.1% | (many diffs) |
| 4 | human | 6569 | SLC34A1 | solute carrier family 34 me... | NM_001167579.2 | 51.7% | 48.9% | (many diffs) |
| 5 | human | 6569 | SLC34A1 | solute carrier family 34 me... | XM_017009774.1 | 50.4% | 50.3% | 0_1ins948;836G>C |
| 6 | human | 6569 | SLC34A1 | solute carrier family 34 me... | XM_017009775.2 | 49.7% | 48.9% | (many diffs) |
| 7 | human | 6569 | SLC34A1 | solute carrier family 34 me... | XR_941112.2 | 47% | (many diffs) | |
| 8 | mouse | 20505 | Slc34a1 | solute carrier family 34 (s... | NM_011392.2 | 86% | 90.7% | (many diffs) |
| 9 | mouse | 20505 | Slc34a1 | solute carrier family 34 (s... | XM_006517177.3 | 73.6% | 77.7% | (many diffs) |
| 10 | mouse | 20505 | Slc34a1 | solute carrier family 34 (s... | XM_006517178.3 | 61.5% | 65.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1983
- ORF length:
- 1917
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtcctacgga gagaggctgg ggtcccctgc tgtctcccca ctcccagtcc 121 gtggggggca tgtgatgcga gggacggcct ttgcctacgt gcccagccct caggtcctac 181 acaggatccc ggggacctct gcctatgcct tccccagcct gggccctgtg gcccttgctg 241 agcacacctg cccctgtggg gaggtcctgg agcgccatga accactgcct gccaagctgg 301 ccctggagga ggagcagaag ccagagtcca ggctggtccc caagctgcgc caggctggcg 361 ccatgctgct caaggtgcca ctgatgctca ccttcctcta cctcttcgtc tgctccctgg 421 acatgctcag ctcggccttc cagctggctg gagggaaggt ggctggtgac atcttcaagg 481 ataacgccat cctgtccaac ccggtggccg ggctggtggt ggggatcctg gtgaccgtgc 541 tggtgcagag ctccagcacc tccacatcca tcatcgtcag catggtctcc tctggcttgc 601 tggaggtgag ctctgccatc cccatcatca tgggctccaa catcggcacc tctgtcacca 661 acaccatcgt ggccctgatg caggcggggg acaggactga cttccggcgg gccttcgcgg 721 gggccacggt gcatgactgc tttaactggc tgtcagtgct ggtcctgctg cccctggagg 781 ctgccactgg ctacctgcac cacatcactc gacttgtggt ggcctccttc aacatccatg 841 gtggccgtga tgctcctgac ctgctcaaga tcatcacaga gcccttcacg aagctcatca 901 tccagctgga cgagtctgtg ataaccagca ttgccactgg tgatgagtcc ctgaggaacc 961 acagtctcat ccagatctgg tgccacccag actccttaca ggctcccacc tccatgtcca 1021 gagcagaggc caactccagc cagacccttg gaaatgccac catggagaaa tgcaaccaca 1081 tctttgtgga cactggccta ccggacctgg ctgtggggct catcctgctg gcaggatccc 1141 tggtgctgct gtgcacctgc ctcatcctcc tagtcaagat gctcaactcc ctgctcaagg 1201 gccaagtggc caaggtcatc cagaaggtca tcaatacgga cttccctgcc cccttcacct 1261 gggtcacagg ctactttgcc atggtggtgg gcgccagcat gaccttcgtg gtccagagca 1321 gttctgtgtt cacctcggcc atcaccccac tcatcggtct tggtgtgatc agcattgaga 1381 gggcctaccc gctcacactg ggttccaaca tcggcaccac caccacggcc atcctggctg 1441 ccctggccag ccccagggag aagctgtcca gcgctttcca gattgccctc tgtcacttct 1501 tcttcaacat ctcgggtatc cttctgtggt acccggtgcc ctgcacacgc ctgcccatcc 1561 gcatggccaa ggcgctgggg aaacgcacgg ccaagtaccg ctggtttgcc gtcctctatc 1621 tccttgtctg cttcctgctg ctgccctcac tggtgtttgg catctccatG GCAGGCTGGC 1681 AGGTCATGGT AGGTGTGGGC ACGCCCTTCG GGGCCCTGCT GGCCTTCGTG GTGCTCATCA 1741 ATGTCCTGCA GAGTCGGAGT CCCGGGCACC TGCCCAAGTG GTTACAGACA TGGGACTTCC 1801 TGCCTCGCTG GATGCACTCC CTGAAGCCCC TGGACCACCT CATCACCCCC GCCACCCTAT 1861 GCTGTGCCAG GCCTGAGCCC CGCTCACCCC CGCTGCCCCC CAGGGTCTTC CTGGAGGAGC 1921 TACCCCCTGC CACACCCTCC CCCCGTCTTG CACTGCCTGC TCACCACAAT GCCACCCGCC 1981 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2041 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2101 GGCTTTATAT ATCTTGTGGA AAGGACGAGT AAGGTGCCAT CCTTACCCCA AGACGCGTTA 2161 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt