Construct: ORF TRCN0000474781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011153.1_s317c1
- Derived from:
- ccsbBroadEn_08617
- DNA Barcode:
- GTTGTCATAGGATTGTCCTTGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG1 (56052)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | NM_019109.5 | 99.8% | 99.5% | 650A>G;800G>A |
| 2 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | XM_017023457.2 | 97% | 96.7% | 650A>G;800G>A;862_863ins39 |
| 3 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | NM_001330504.1 | 75.9% | 75.6% | 0_1ins333;317A>G;467G>A |
| 4 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | XM_017023458.1 | 75.9% | 75.6% | 0_1ins333;317A>G;467G>A |
| 5 | human | 56052 | ALG1 | ALG1 chitobiosyldiphosphodo... | XR_932882.3 | 71.7% | (many diffs) | |
| 6 | human | 650368 | TSSC2 | tumor suppressing subtransf... | NR_024248.1 | 48% | (many diffs) | |
| 7 | human | 644974 | ALG1L2 | ALG1 chitobiosyldiphosphodo... | NM_001136152.1 | 43.1% | 39.4% | (many diffs) |
| 8 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005844.2 | 34.6% | 32.5% | (many diffs) |
| 9 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005845.1 | 34.6% | 32.5% | (many diffs) |
| 10 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | NM_001195223.1 | 34.5% | 32.5% | (many diffs) |
| 11 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005846.2 | 31.1% | 29.6% | (many diffs) |
| 12 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_017005847.1 | 31.1% | 29.6% | (many diffs) |
| 13 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | NM_001015050.2 | 31% | 29.6% | (many diffs) |
| 14 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_024453383.1 | 25.6% | 24.7% | (many diffs) |
| 15 | human | 644974 | ALG1L2 | ALG1 chitobiosyldiphosphodo... | XM_024453711.1 | 25.6% | 20.8% | (many diffs) |
| 16 | human | 200810 | ALG1L | ALG1 chitobiosyldiphosphodo... | XM_024453384.1 | 25.5% | 24.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1458
- ORF length:
- 1392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcctcatgc ttggtcctgc tggcgctgtg tctgctgctg ccgctgctgc 121 tgctgggagg atggaagcgc tggcgccggg ggcgggcggc ccggcatgta gtagcggtgg 181 tgctgggcga cgtgggccgc agcccccgta tgcagtacca cgcgctgtcg ttggccatgc 241 acggcttctc ggtgaccctc ctggggttct gcaactccaa accccatgat gagctcttgc 301 agaacaacag aattcagatt gtggggttga cagaacttca gagtcttgca gttgggcccc 361 gagttttcca gtacggagtc aaagttgtac ttcaggctat gtacttgctg tggaagttga 421 tgtggaggga gccaggtgcc tatatctttc tccagaaccc cccaggtctg cctagcattg 481 ctgtctgctg gttcgtgggc tgcctttgtg gaagcaagct cgtcattgac tggcacaact 541 atggctactc catcatgggt ctggtgcatg gccccaacca tcccctcgtt ctgctggcca 601 agtggtacga gaagttcttt gggcgcctgt cccacctgaa cctgtgtgtt accaatgcta 661 tgcgagaaga cctggcggat aactggcaca tcagggctgt gaccgtctac gacaggcccg 721 catctttctt taaagagaca cctctggacc tgcagcaccg gctcttcatg aagctgggca 781 gcatgcactc tccgttcagg gcccgctcag aacctgagga cccagtcacg gagcggtcgg 841 ccttcacgga gcgggatgct gggaacgggc tggtgacgcG TCTCCGTGAG CGGCCAGCCC 901 TGCTGGTCAG CAGCACGAGC TGGACAGAGG ACGAAGACTT CTCCATCCTG CTGGCAGCTT 961 TAGAAAAGTT TGAACAACTG ACTCTTGATG GACACAACCT TCCTTCTCTC GTCTGTGTGA 1021 TAACAGGCAA AGGGCCTCTG AGGGAGTATT ATAGCCGCCT CATCCACCAG AAGCACTTCC 1081 AGCACATCCA GGTCTGCACC CCCTGGCTGG AGGCCGAGGA CTACCCCCTG CTTCTAGGGT 1141 CGGCGGACCT GGGTGTCTGT CTGCACACGT CCTCCAGTGG CCTGGACCTG CCCATGAAGG 1201 TGGTGGACAT GTTCGGGTGC TGTTTGCCTG TGTGTGCTGT GAACTTCAAG TGTTTACATG 1261 AGCTGGTGAA ACATGAAGAA AATGGCCTGG TCTTTGAGGA CTCAGAGGAA CTGGCAGCTC 1321 AGCTGCAGAT GCTTTTCTCA AACTTTCCTG ATCCTGCGGG CAAGCTAAAC CAGTTCCGGA 1381 AGAACCTGCG GGAGTCGCAG CAGCTCCGAT GGGATGAGAG CTGGGTGCAG ACTGTGCTCC 1441 CTTTGGTTAT GGACACATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGTTGTCA TAGGATTGTC 1621 CTTGGGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt