Construct: ORF TRCN0000474787
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018906.3_s317c1
- Derived from:
- ccsbBroadEn_14906
- DNA Barcode:
- ATATACCGGGATTACACATCTTGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CASK (8573)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474787
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_001126054.2 | 98.5% | 26.1% | (many diffs) |
| 2 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_001126055.2 | 98.4% | 26.1% | (many diffs) |
| 3 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543995.2 | 97.7% | 25.9% | (many diffs) |
| 4 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_006724566.3 | 97% | 26.5% | (many diffs) |
| 5 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_001367721.1 | 96.6% | 25.3% | (many diffs) |
| 6 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543996.2 | 96.4% | 26.2% | (many diffs) |
| 7 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543994.2 | 96.1% | 25.6% | (many diffs) |
| 8 | human | 8573 | CASK | calcium/calmodulin dependen... | NM_003688.3 | 96.1% | 25.5% | (many diffs) |
| 9 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_005272686.4 | 95.9% | 25.5% | (many diffs) |
| 10 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543993.2 | 95.3% | 25.2% | (many diffs) |
| 11 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_024452473.1 | 76.6% | 5.6% | (many diffs) |
| 12 | human | 8573 | CASK | calcium/calmodulin dependen... | XM_011543997.3 | 76% | 5.4% | (many diffs) |
| 13 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_006527561.3 | 93.7% | 26.1% | (many diffs) |
| 14 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318369.1 | 93.6% | 25.7% | (many diffs) |
| 15 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_009806.3 | 93.5% | 26.1% | (many diffs) |
| 16 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318370.1 | 93.1% | 25.8% | (many diffs) |
| 17 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247441.2 | 92.9% | 25.9% | (many diffs) |
| 18 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318371.1 | 92.3% | 26% | (many diffs) |
| 19 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247440.2 | 92.2% | 25.8% | (many diffs) |
| 20 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247439.2 | 92.1% | 25.4% | (many diffs) |
| 21 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_001284503.1 | 91.9% | 25.3% | (many diffs) |
| 22 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_001284504.1 | 91.7% | 26.7% | (many diffs) |
| 23 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318368.1 | 91.6% | 25.5% | (many diffs) |
| 24 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_006527560.3 | 91.4% | 25.5% | (many diffs) |
| 25 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247438.2 | 91.3% | 25.1% | (many diffs) |
| 26 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | NM_001284505.1 | 91.2% | 25.5% | (many diffs) |
| 27 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318367.1 | 90.8% | 25.2% | (many diffs) |
| 28 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247436.2 | 89.6% | 24.6% | (many diffs) |
| 29 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_017318366.1 | 89% | 24.8% | (many diffs) |
| 30 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247437.1 | 88.4% | 24.9% | (many diffs) |
| 31 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247435.2 | 88.2% | 24.3% | (many diffs) |
| 32 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247433.1 | 88.1% | 24.3% | (many diffs) |
| 33 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247431.1 | 87.5% | 24% | (many diffs) |
| 34 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247432.1 | 87.1% | 24.1% | (many diffs) |
| 35 | mouse | 12361 | Cask | calcium/calmodulin-dependen... | XM_011247434.1 | 86.9% | 24.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 777
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgacgac gacgtgctgt tcgaggatgt gtacgagctg tgcgaggtga 121 tcggaaaggg tcccttcagt gttgtacgac gatgtatcaa cagagaaact gggcaacaat 181 ttgctgtaaa aattgttgat gtagccaagt tcacatcaag tccagggtta agtacagaag 241 atctaaagcg ggaagccagt atctgtcata tgctgaaaca tccacacatt gtagagttat 301 tggagacata tagctcagat ggaatgcttt acatggtttt cgaatttatg gatggagcag 361 atctgtgttt tgaaatcgta aagcgagctg acgctggttt tgtgtacagt gaagctgtag 421 ccagccatta tatgagacag atactggaag ctctacgcta ctgccatgat aataacataa 481 ttcacaggga tgtgaagccc cactgtgttc tccttgcctc aaaagaaaac tcggcacctg 541 ttaaacttgg aggctttggg gtagctattc aattagggga gtctggactt gtagctggag 601 gacgtgttgg aacacctcat tttatggcac cagaagtggt caaaagagag ccttacggaa 661 agcctgtaga cgtctggggg tgcggtgtga tcctttttat cctgctcagt ggttgtttgc 721 ctttttacgg aaccaaggaa agattgtttg aaggcattat taaaggaaaa tatagatgaa 781 tccaaggcag tggagccata tctctgaaag tgccaagacc tagtacgtcg catgctgatg 841 ctggatccag ctgaaaggat ccactgtttt atgaagccac tgaatcaccc atggcttaag 901 gagcgggatc gttacgccta caagattcat cttccagaaa cagtagagca gctgaggaaa 961 ttcaatgcaa ggaggaaact aaagggtgca gtactagccg ctgtgtcaag tcacaaattc 1021 aactcattct atggggatcc ccctgaagag ttaccagatt tctccgaaga ccctacctcc 1081 tcaggacttc tagcagcaga aagagcagtc tcacaggtgc tggacagcct ggaagagatt 1141 catgcgctta cagactgcag tgaaaaggac ctagattttc tacacagtgt tttccaggat 1201 cagcatcttc acacactact agatctgtat gacaaaatta acacaaagtc ttcaccacaa 1261 atcaggaatc ctccaagcga tgcagtacag agagccaaag aggtattgga agaaatttca 1321 tgttaccctg agaataacga cgcaaaggaa ctaaagcgta ttttaacaca acctcatttc 1381 atggccttac ttcagactca cgacgtagtg gcacatgaag tttacagtga tgaagcattg 1441 agggtcacac ctcctcccac ctctccctat ttaaacggcg attctccaga aagtgctaac 1501 ggagacatgg atatggagaa tgtgaccaga gttcggctgg tacagtttca aaagaacaca 1561 gatgaaccaa tgggaatcac tttaaaaatg aatgaactaa atcattgtat tgttgcaaga 1621 attatgcatg ggggcatgat tcacaggcaa ggtacacttc atgttggtga tgaaattcga 1681 gaaatcaatg gcatcagtgt ggctaaccaa acagtggaac aactgcaaaa aatgcttagg 1741 gaaatgcggg ggagtattac cttcaagatt gtgccaagtt accgcactca gtcttcgtcc 1801 tgtgaggact tgccatcaac tacccaacca aaaggacgac agatctatgt aagagcacaa 1861 tttgaatatg atccagccaa ggatgacctc atcccctgta aagaagctgg cattcgattc 1921 agagttggtg acatcatcca gattattagt aaggatgatc ataattggtg gcagggtaac 1981 ctgaaaaact cccaaaaatt ggactgcagt ctcattcctt cttcctgact ttcaggaatt 2041 gcgagtagct tgcatgncca tggagagacc caaacaggag cagcagccca gctgtacttg 2101 tttgcaagaa aaaagaagca gtacaaagat aaatatttgg caaagcacaa tgcagtgttn 2161 gatcaattag atcttgtcac atatgaagaa gtagtaaaac tgccagcatt caagaggaaa 2221 acactagtct tattaggcgc acatggtgtt gggagaagac acataaaaaa cactctcatc 2281 acaaagcacc cagaccggtt tgcgtaccct attccacata caaccagacc TCCAAAGAAA 2341 GACGAAGAAA ATGGAAAGAA TTATTACTTT GTATCTCATG ACCAAATGAT GCAAGACATC 2401 TCTAATAACG AGTACTTGGA GTACGGCAGC CACGAGGATG CGATGTATGG GACAAAACTG 2461 GAGACCATCC GGAAGATCCA CGAGCAGGGG CTGATTGCAA TACTGGACGT GGAGCCTCAG 2521 GCACTGAAGG TCCTGAGAAC TGCAGAGTTT GCTCCTTTTG TTGTTTTCAT TGCTGCACCA 2581 ACTATTACTC CAGGTTTAAA TGAGGATGAA TCTCTTCAGC GTCTGCAGAA GGAGTCTGAC 2641 ATCTTACAGA GAACATATGC ACACTACTTC GATCTCACAA TTATCAACAA TGAAATTGAT 2701 GAGACAATCA GACATCTGGA GGAAGCTGTT GAGCTCGTGT GCACAGCCCC ACAGTGGGTC 2761 CCTGTCTCCT GGGTCTATTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 2821 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 2881 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATATAC CGGGATTACA 2941 CATCTTGCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt