Construct: ORF TRCN0000474799
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011290.1_s317c1
- Derived from:
- ccsbBroadEn_00958
- DNA Barcode:
- TGCACTATCACAGGATTGACTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAB21L1 (4081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474799
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4081 | MAB21L1 | mab-21 like 1 | NM_005584.5 | 100% | 100% | |
| 2 | human | 10586 | MAB21L2 | mab-21 like 2 | NM_006439.5 | 79.6% | 94.1% | (many diffs) |
| 3 | mouse | 17116 | Mab21l1 | mab-21-like 1 (C. elegans) | NM_010750.3 | 90.2% | 100% | (many diffs) |
| 4 | mouse | 23937 | Mab21l2 | mab-21-like 2 | NM_011839.4 | 78.7% | 93.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgcggcccag gccaagctgg tctaccatct gaataaatac tacaacgaaa 121 aatgccaagc caggaaagct gccattgcca aaactatccg ggaagtctgc aaagtagttt 181 ccgacgtact gaaggaagtg gaagtgcagg agccgcggtt catcagctct ctcaacgaga 241 tggacaatcg ctacgagggc ctcgaggtca tctcccccac cgaatttgaa gtggtgcttt 301 atctcaacca aatgggggtg ttcaacttcg tggacgatgg ctcactgccc ggctgcgcgg 361 tgctgaagtt gagcgacggg cgcaagagga gcatgtccct ctgggtggaa ttcattaccg 421 cctccggcta cctctcggcg cgcaaaatcc ggtccaggtt tcagacgctg gtggctcaag 481 cggtagacaa atgtagctac cgggatgtgg taaagatggt ggcagacacc agcgaagtga 541 aactgagaat ccgagatagg tacgtggtgc agatcacgcc ggcctttaaa tgcaccggga 601 tctggccgag gagtgctgcc cactggccac ttccccacat cccctggccg ggacccaacc 661 gggtggcgga ggtcaaggcg gaaggtttca atctcttgtc caaggagtgc cactccttgg 721 ccggcaagca gagctcggcg gagagcGACG CCTGGGTGCT GCAGTTCGCG GAGGCAGAGA 781 ACAGACTGCA GATGGGGGGC TGCAGAAAGA AGTGCCTCTC CATCCTCAAA ACCTTAAGGG 841 ATCGTCACCT TGAACTGCCG GGCCAGCCCT TGAACAATTA CCATATGAAG ACTCTGGTTT 901 CCTACGAGTG TGAAAAGCAT CCCCGAGAGT CGGACTGGGA CGAGTCTTGC CTGGGTGATC 961 GGCTGAACGG GATTTTGCTG CAACTTATCT CCTGCCTGCA GTGCCGGCGG TGTCCCCACT 1021 ACTTTCTACC GAACTTAGAT CTGTTTCAAG GCAAACCTCA CTCAGCTCTG GAAAACGCTG 1081 CCAAACAAAC GTGGCGACTG GCAAGAGAGA TCCTGACCAA CCCGAAAAGT TTGGAAAAAC 1141 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGATG CACTATCACA GGATTGACTA ACACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt