Construct: ORF TRCN0000474827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001514.1_s317c1
- Derived from:
- ccsbBroadEn_03819
- DNA Barcode:
- TTGTGGGATATCTGCACCTATCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C3orf14 (57415)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57415 | C3orf14 | chromosome 3 open reading f... | NM_001291941.2 | 100% | 100% | |
| 2 | human | 57415 | C3orf14 | chromosome 3 open reading f... | NM_001291942.2 | 100% | 100% | |
| 3 | human | 57415 | C3orf14 | chromosome 3 open reading f... | NM_020685.5 | 100% | 100% | |
| 4 | human | 57415 | C3orf14 | chromosome 3 open reading f... | XM_017006931.1 | 100% | 100% | |
| 5 | human | 57415 | C3orf14 | chromosome 3 open reading f... | NM_001291943.1 | 59.9% | 43.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 450
- ORF length:
- 384
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ttccttgttt gctcaagaaa ttcgcctttc taaaagacat gaagaaatag 121 tatcacaaag attaatgtta cttcaacaaa tgGAGAATAA ATTGGGTGAT CAACACACAG 181 AAAAGGCATC TCAACTCCAA ACTGTTGAGA CTGCTTTTAA AAGGAACCTT AGTCTTTTAA 241 AGGATATAGA AGCAGCAGAA AAGTCACTAC AGACCAGGAT TCACCCACTT CCACGGCCTG 301 AGGTGGTTTC TCTTGAGACT CGTTACTGGG CATCAGTAGA AGAATATATT CCCAAATGGG 361 AACAGTTTCT TTTAGGAAGA GCACCATATC CTTTTGCTGT TGAAAATCAA AATGAAGCAG 421 AAAATACCAT TCAAAATGAG GCACAGCGAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTGTGG GATATCTGCA CCTATCGCAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt